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蔓性千斤拔總黃酮的提取與分析A1A3A0A4A5A2A6A0A7A8A9A10A11A12A13A14A15A16A15A17A18A19A20A21A14A15A17A22A23A24A25A26A27A28A29A18A19A10A30A30A30A31A32A33A34A11A35A36A22A23A24A25A37A4A35A36A38A39A40A41A30A30A34A41A42A43A42A43A44A45A46A47A48A49A50A51A52A53A54A55A56A57A58A59A60A61A62A63A64A65A66A67A68A69A70A71A72A73A74A75A76A62A77A78A79A80A81A82A83A47A84A85A86A87A88A89A90A91A92A93A94A95A96A97A98A99A100A101A102A103A104A105A106A107A108A109A110A111A112A113A114A95A96A115A116A4A117A118A119A120A121A122A106A107A123A124A125A126A127A128A129A130A131A132A133A134A135A136A137A138A139A140A67A84A85A141A142A80A143A70A59A60A61A62A144A145A67A128A146A76A147A148A142A149A150A151A152A153A81A154A155A47A15685A157A158A159A160A161A162140A159A163A16420MINA159A165A1661A167A168A169A170A171A172A173A174A175A159A176A177A178A160A179A180A181A182A183A184A185A186A187A188A189A190A191A181498A192A193A194A195A195A196A197A167A198A177A163A164A199A165A166A200A201A202A187A188A189A159A165A166A203A191A204A205A206A207A165A166A159A208A209A210A211A212A213A178A160A179A180A181A182A183A184A185A186A200A201A202A187A188A189A168A214A215A216A217A159A218A216A217A219A220A221A222A159A223A224A225A193A226A227A228A226A227A228A229A229A200A201A202A230A187A188A189A230A165A166A230A163A164A217A230A231A232A233A221A234A214A235A235A236A217A230A204A203A161A237A238A239EXTRACTIONANDANALYSISOFTOTALFLAVONOIDSFROMFLEMINGIAPHILIPPINENSISHUALI1ANDXIAOJUNMA1,21INSTITUTEOFMEDICINALPLANTDEVELOPMENT,CHINESEACADEMYOFMEDICALSCIENCESANDPEKINGUNIONMEDICALCOLLEGE,BEIJING100094,CHINA2GUANGXIBOTANICALGARDENOFMEDICINALPLANT,NANNING530023,CHINAABSTRACTOBJECTIVETOINVESTIGATETHEOPTIMUMEXTRACTIONCONDITIONANDASIMPLEEFFECTIVEANALYSISMETHODOFTOTALFLAVONIODFROMFLEMINGIAPHILIPPINENSISMETHODSOLELYSOLVENTEXTRACTION,ORTHOGONALEXPERIMENTANDCOMPARISONWITHHEATINGREFLUXEXTRACTIONWEREEMPLOYEDTOINVESTIGATETHEEXTRACTIONCONDITIONOFTOTALFLAVONOIDWITHMATHANOLUSINGSUPERSONICWAVEFROMFPHILIPPINENSISTHETOTALFLAVONOIDCONTENTWASDETERMINEDBYRUTINANDGENSTEINSTANDARDSAMPLESANDTHERESULTSWASAIDEDTODETERMINEBYHPLCRESULTSTHEYIELDOFTOTALFLAVONOIDWAS498USINGGENISTEINSTANDARDSAMPLEATTHEOPTIMUMCONDITIONSOF85METHANOLSOLUTION,SOLIDLIQUIDRATIO140,40MIN,1TIMEFORSUPERSONICEXTRACTIONCONCLUDSIONITWASTHEFIRSTTIMETOEXTRACTTOTALFLAVONOIDFROMFPHILIPPINENSISUSINGULTRASONICWAVEWHICHWASBETTERTHANREFLEXEXTRACTIONTHEANALYSISMETHOD,ULTRAVIOLETSPECTROPHOTOMETRYWITHTHEGENISTEINASSTANDARDSAMPLE,WASESTABLISHEDITWASSIMPLE,FEASIBLEANDEASYTOOPERATEA240A241A242A243A244A245A246A247A248A249A250A251A252A253A254A255A33A0A1A243A244A138A2A3A4A5A2A2A6A141A32A7A8A9A144A10A11A12A98A13A14A15A16A147A17A18A98A19A2A4A2A141A151A32A5A4A6A20A4A2A152A16A153A141A154A21A22A18A98A157A23A154A21A24A25A26A27A18A22A28A29A27A30A21A29A31A17A30A29A28A31KEYWORDSFLEMINGIAPHILIPPINENSISTOTALFLAVONOIDEXTRACTIONSUPERSONICWAVEULTRAVIOLETVISIBLESPECTROPHOTOMETRYHPLCA167A34A62A35A36A150FLEMINGIAPHILIPPINENSISMERRETROLFEA37A38A169A62A35A36A38A89A39A40A62A35A36A41A42A43A38A172A44A45A46A47A109A48A49A50A51A177A52A51A53A54A51A55A56A38A181A57A58A109A89A59A38A184A46A47A174A60A61A63A64A65A66A38A192A67A68A69A38A196A34A70A67A38A71A72A73A74A201A75A40A76A771,2A38A78A89A79A80A81A82A83A84A85A86A87A38A112A42A43A52A88A90A91A92A93A94A43A89A46A47A95A34A43A96A83A130A97A38A167A34A62A35A36A216A99A60A100A101A174A88A102A103A104A105A106A107A38A89A102A74A108A88A110A111A113A82A112A42A43A114A115A38A44A173A116A82A117A118A119A120A121A62A35A36A88A122A90A91A93A94A43A83A117A118A123A120A121A42A43A52A88A61A124A101A109A125A126A127A128A38A120A129A131A132A38A232A133A134A38A234A135A93A94A43A88A136A137A139A140A239A240A142A143A83A145A243A172A109A146A122A90A91A88A148A149A52A38A117A118A123A120A121A155A156A155A101A89A74A158A46A159A88A160A161A1621A163A164A164A165A166A165A166A165A165A166A168A168A170A171A175A176A17811A165A166A165A166A179A179A180A182A183A185A186A187A188A189A1902006A2484A249A191A193A194A195A197A198A199A200A250A202A20340A204A205A206A254A207A208A209A210A211A212A213A214A215A217A218A219A220A221A200A222A223A224F20060526A225A206A226A227A228A229A208A230A231A233A235A236A225A23712A238A241A241A242A242WATERS2690996A244A245A246A247A251A252A253A255A118A0A13A93A14A150DADA152A255EMPOWERA251A252A124A1A15A31722A109A2A2A3A136A4A16A5A6A7A109A8A18A9A31A10A11A109A2A2A3A136A4A12A17A12A19A19A20A21A22A23A9A2413A25A26A26A27A27A28A29A30A32A33A29A30A34A33A35A36A211A32A33A57A37A33A38A37A33A39A44A40A41A30A33A42A89A43A45A46A47A38A48A33A89A49A50A46A51HONEYWELLA52A53A54A55A56A56A58A59A60A61A62A63A64A52A652A66A67A68A69A68A6921A70A71A72A73A74A75A76A77A72A73A74A75A78A79A80A81A82A83A84A85A86A87A88A90100MGA91A10310MLA92A94A95A96A97A98A99A100A101A102A104A105A92A106A107A122A97A108A110A111A219A112A122A1131MG/MLA87A114A115A116A101A117A119A78A79A80A81A82A83A84A85A86A87A120A121A123A125636MGA91A10310MLA92A94A95A96A97A98A99A100A101A102A104A105A92A106A107A122A97A108A110A111A219A112A122A1130636MG/MLA87A114A115A116A101A117A11922A126A127A128A129A130A131A128A129A130A131221A132A133A134A126A127A128A129A132A133A134A126A128A129A135A137A138A1393A140A141A142A143A144A145A146A147A14800A17701A17702A177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