神經(jīng)膠質(zhì)細胞文獻回報ppt課件

1、Evidence for NG2-glia Derived, Evidence for NG2-glia Derived, Adult-Born FunctionalAdult-Born FunctionalNeurons in the HypothalamusNeurons in the HypothalamusSarah C. Robins1, Eric Trudel2, Olivia Rotondi1, Xiaohong Liu1, Tina Djogo1, Darya Kryzskaya1,Charles W. Bourque2, Maia V. Kokoeva1*匯報人：學號：;思緒

2、： 有文獻報道，鼠科動物下丘腦有生成神經(jīng)元的功能，但是是哪種細胞具有這種功能？進展鑒定 腦室膜細胞ventricular tanycytes被鑒定為是神經(jīng)元生成的來源，但是研討發(fā)現(xiàn)，由此產(chǎn)生的神經(jīng)元占總新神經(jīng)元的很少部分，另一個來源是？ 有報道下丘腦細胞能產(chǎn)生具有神經(jīng)元標志物NeuN or HuC/D (Hu)的細胞,但是缺乏電生理的證明? Rivers et al用基因命運跟蹤技術(shù)證明表達PDGFRa的細胞能在成年鼠的梨狀皮層中產(chǎn)生投射神經(jīng)元(但是其他人用Olig2-CreER or PDGFRa-CreER mice不能證明此結(jié)果或提出質(zhì)疑), 那么NG2-glia是不是也可以產(chǎn)生neur

3、on？; 這篇文章主要是運用轉(zhuǎn)基因技術(shù),失蹤技術(shù),對下丘腦中NG2-glia進展不同時期的察看,并對最終分化而成的的帶有神經(jīng)元marker的細胞進展電生理鑒定,最終闡明NG2-glia是成體下丘腦中新生神經(jīng)元的主要來源.;NG2-glia:NG2-glia是以硫酸軟骨素蛋白聚醣(NG2)和血小板衍生物生長因子受體alpha(PDGFRa)為標志物的神經(jīng)膠質(zhì)細胞;占成體中樞神經(jīng)系統(tǒng)細胞的10%;可以分裂增殖,產(chǎn)生少突膠質(zhì)細胞;有文章報道,其在梨狀皮層(piriform cortex, 和下丘腦一樣都屬于邊緣系統(tǒng))中能產(chǎn)生少量神經(jīng)元,但是也有文章對此質(zhì)疑;A為腦室灌注一次24h后,下丘腦實體成對出

4、現(xiàn)BrdU+的細胞,闡明由遷徙而來的能夠性不大;BC可以看出分裂細胞細胞廣泛分布于下丘腦,沒有富集景象;BD是七天灌注后立刻取樣察看和28天后察看的對比無明顯分布，闡明這些細胞的不是遷徙來的；E顯示BrdU+ cell isNG2-glia下丘腦中存在大量增值細胞，而非遷移過來的，大部分是NG2-glia;有報道，Sox2+ cell能分化為神經(jīng)元，那么NG2-glia假設(shè)能分化為神經(jīng)元，也能夠產(chǎn)生Sox2A:Sox2+ cells主要分布在第三腦室附近和下丘腦實體；CDE:三種雙陽性類型；ABF：37%+2.8%NG2+、Sox2+雙標志，呈均勻分布，NG2是主要的增值細胞; Co-labe

5、lling with NG2 and Sox2 antibodies indicated that 37.0% +2.8 of hypothalamic NG2-glia robustly express Sox2 (Fig. 2A,B,F). Despite the fact that NG2-glia accounted for fewer than 10% of the total Sox2+ cell population, almost all (92.2%+2.8) of the hypothalamic BrdU+/Sox2+ cells also expressed NG2 (

6、Fig. 2A,B,F), suggesting that NG2-glia are the only substantially proliferative subpopulation of Sox2+ cells in the adult hypothalamic parenchyma. three types of NG2-glia pairs according to Sox2 expression: i) substantial Sox2 expression in both cells; ii) no to barely detectable Sox2 expression in

7、both cells; or iii) Sox2 expression limited to one cell (Fig. 2CE), relate to differences in neural fates.;BC：NG2是下丘腦主要的增值細胞；D-F：30days postTX 還有NG2細胞表達；L：7天和60天后，雙陽性的絕度數(shù)量一樣，2-7天少突膠質(zhì)細胞迅速增多為重組得的保真性提供根底；GH：外表不表達星形膠質(zhì)細胞的marker，即不分化為星形膠質(zhì)細胞；LM：Tom+細胞中：NG2+數(shù)量和比率減少，而APC+相反; More than two thirds of the detec

8、ted BrdU+/EdU+ cells expressed NG2 (Fig. 3B,C), indicating that adult hypothalamic NG2-glia can undergo multiple self-renewing divisions, in line with findings for the cerebral cortex 11； one of the primary functions of adult hypothalamic NG2-glia is to provide a continuous supply of new oligodendro

9、cytes；;Figure S1 Fidelity of the NG2creER:tdTomato reporter. Mice were treated with TX twice daily for two days. (A) Cell types were determined by colocalisation of Tom with antibodies to NG2 to identify NG2-glia (arrowhead N), CD13 to identify pericytes (arrowhead P) and APC to identify oligodendro

10、cytes (arrowhead O). (B) Quantification of results from A. Labelling with antibodies to HuC/D (C) and NeuN (D) confirmed that Tom+ neurons were not present at this timepoint.重組實驗的保真性研討：周皮細胞和已分化神經(jīng)細胞不表達tom;A-C：tom+和HuC/D+細胞被檢出；C-D：a-tom+和NeuN+被檢出；G：雙標志細胞隨時間呈線性增長；H：tom+中Hu+和Neu+的比率不同，代表的時期不同，不同的偏好等緣由;A：第一次注射TX，后繼續(xù)飲用含有BrdU的水直到，處死取樣，發(fā)現(xiàn)Hu+/Tom+/BruU+的三標細胞；B： Hu+/Tom+中BruU+的比率和tom+中BruU+的比率沒有明顯不同這組實驗闡明NG2-glia分化為了神經(jīng)