BALB_c小鼠腎缺血再灌注損傷模型的建立與評價_胡紅林

1、中國組織工程研究與臨床康復 第15卷 第5期 20110129出版Journal of Clinical Rehabilitative Tissue Engineering Research January 29, 2011 Vol.15, No.5BALB/c小鼠腎缺血再灌注損傷模型的建立與評價胡紅林1，王共先2，鄒 叢3，習小慶1，史子敏1Establishment and evaluation of renal ischemia/reperfusion injury models in BALB/c miceHu Hong-lin1, Wang Gong-xian2, Zou Cong3

2、, Xi Xiao-qing1, Shi Zi-min1AbstractDepartment of Urinary Surgery, Second AffiliatedHospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; 2Department ofUrinary Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; 3Department of End

3、ocrinology, Fourth AffiliatedHospital of Nanchang University, Nanchang 330003, Jiangxi Province, ChinaHu Hong-lin, Doctor, Attending physician, Department of Urinary Surgery, Second AffiliatedHospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaCorrespondence to: Hu Hong-lin, Doc

4、tor, Attending physician, Department of Urinary Surgery, Second AffiliatedHospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaReceived: 2010-07-12 Accepted: 2010-09-12BACKGROUND: For studies of some drugs, mouse serves as an ideal animal for model establishment. However, the fai

5、lure rate is high due to poor toleration, small size of the kidney and renal pedicle.OBJECTIVE: To construct the model of renal ischemia/reperfusion injury (IRI) in BALB/c mice and evaluate influences of different ischemic time on IRI.METHODS: Renal arteries of mice were bilaterally clamped with mic

6、ro-artery clamps to establish model of renal IRI in maleBALB/c mice. According to different ischemic time, the mice were divided into four groups: 0 (control), 30, 35, and 45 minutes. The animals were sacrificed at 24 hours post-operatively. Renal function and pathology of the kidneys were examined.

7、 The situation of illness and survival in 45-minute group were observed.RESULTS AND CONCLUSION: The successful rate of model was 95.9%. Levels of serum creatinine and blood urea nitrogen as well as histological scores were remarkably increased in 30-, 35-, and 45-minute groups compared with control

8、group 24 hours after operation (P < 0.05). However, the survival rate was significantly lower in 45-minute group (P < 0.05). Results show that stable model of renal IRI can be gained in BALB/c mice by applying micro-artery clamp to incarcerate bilateral renal arteries. The optimal renal ischem

9、ia time is 35-45 minutes in male mice.摘要背景：對于一些藥物研究，小鼠是理想的造模工具，但由于小鼠耐受性相對較差，腎臟及腎蒂小且難于尋找，容易增加實驗誤差，導致造模失敗。目的：探討B(tài)ALB/c小鼠腎缺血再灌注損傷模型的建立方法，評價腎臟缺血時間對腎缺血再灌注損傷的影響。方法：采用微型動脈夾夾閉小鼠雙側(cè)腎蒂的方法建立雄性BALB/c小鼠腎缺血再灌注損傷模型，根據(jù)腎缺血時間不同分為 0 min組(對照組)、30 min組、35 min組、45 min組，腎再灌注后24 h觀察腎功能和腎臟病理組織學的變化，比較不同的腎臟缺血時間對上述指標的影響；觀察45 min

10、組小鼠腎缺血再灌注損傷后的生存率。結(jié)果與結(jié)論：模型成功率95.9%，與對照組相比，腎缺血30 min組、35 min組和45 min組再灌注后24 h血清肌酐、尿素氮和腎臟病理組織學評分均升高，腎缺血45 min組生存率明顯下降，差異均有顯著性意義(P < 0.05)。結(jié)果提示，應用微型動脈夾夾閉小鼠雙側(cè)腎蒂的方法可制備穩(wěn)定腎缺血再灌注損傷模型，雄性小鼠腎缺血3545 min是造模較為理想的腎缺血時間，所得模型效果滿意。0 引言 腎缺血再灌注損傷(renal ischemia- reperfusion injury，IRI)是缺血性急性腎功能衰竭的主要發(fā)病機制，具有較高的發(fā)病率和死亡率1-2，伴隨著一系列連貫的細胞事件發(fā)生，包括活性氧(ROS)釋放、凋亡、壞死、炎癥細胞的浸潤和活性介質(zhì)的釋放，從而導致組織損傷。動物實驗是開展腎IRI研究的常用方法，合格的動物模型建立是實驗研究成功的基礎和關鍵。作者應用微型動脈夾夾閉雙側(cè)腎蒂方法制備BALB/c小鼠IRI模型，取得較為滿意的效果，并觀察了不同的腎臟缺血時間對腎IRI的影響。31 材料和方法設計：單一樣本觀察。時間及地點：實驗