熒光染料激發(fā)和發(fā)射光譜表

1、熒光素名稱,激發(fā)波長 (nm),發(fā)射波長 (nm),備注1,5 IAEDANS,336,490,1,8-ANS,372,480,4-Methylumbelliferone,385,502,5-carboxy-2,7-dichlorofluorescein,504,529,5-Carboxyfluorescein (5-FAM),492,518,5-Carboxynapthofluorescein (pH 10),512/598,563/668,Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA),542,568,5-FAM (5-Carbox

2、yfluorescein),492,518,5-HAT (Hydroxy Tryptamine),370-415,520-540,5-Hydroxy Tryptamine (HAT),370-415,520-540,5-ROX (carboxy-X-rhodamine),578,604,567,591,5-TAMRA (5-Carboxytetramethylrhodamine),548,552,542,568,6-Carboxyrhodamine 6G,518,543,6-CR 6G,518,543,6-JOE,520,548,7-Amino-4-methylcoumarin,351,430

3、,7-Aminoactinomycin D (7-AAD),546,647,7-Hydroxy-4-methylcoumarin,360,449, 455,9-Amino-6-chloro-2-methoxyacridine,412, 430,471, 474,ABQ,344,445,Acid Fuchsin,540,630,ACMA (9-Amino-6-chloro-2-methoxyacridine),412, 430,471, 474,Acridine Orange + DNA,502,526,Acridine Orange + RNA,460,650,Acridine Orange,

4、 both DNA & RNA,440-480,520-650,Acridine Red,455-600,560-680,Acridine Yellow,470,550,Acriflavin,436,520,Acriflavin Feulgen SITSA,355-425,460,Aequorin (Photoprotein),466,PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP,Alexa Fluor 350,346,442,342,441,Alexa Fluor

5、 430,431,540,Alexa Fluor 488,495, 492,519, 520,Alexa Fluor 532,531, 532,553, 554,Alexa Fluor 546,556, 557,572, 573,Alexa Fluor 568,577, 578,603,Alexa Fluor 594,590, 594,617, 618,Alexa Fluor 633,632,650,Alexa Fluor 647,647,666,Alexa Fluor 660,668,698,Alexa Fluor 680,679,702,Alizarin Complexon,530-560

6、, 580,580, 624-645,Alizarin Red,530-560,580,Allophycocyanin (APC),630, 645,655, 660,AMC, AMCA-S,345,445,AMCA (Aminomethylcoumarin),345,425,347,444,AMCA-X,353,442,Aminoactinomycin D,555,655,Aminocoumarin,346,442,350,445,Aminomethylcoumarin (AMCA),345,425,347,444,Anilin Blue,600,Anthrocyl stearate,360

7、-381,446,APC (Allophycocyanin),630, 645,655, 660,APC-Cy7,625-650,755,APTRA-BTC = Ratio Dye, Zn2+,466/380,520/530,Ratio Dye, Zn2+APTS,424,505,Astrazon Brilliant Red 4G,500,585,Astrazon Orange R,470,540,Astrazon Red 6B,520,595,Astrazon Yellow 7 GLL,450,480,Atabrine,436,490,ATTO-TAG CBQCA,465,560,ATTO-

8、TAG FQ,486,591,Auramine,460,550,Aurophosphine G,450,580,Aurophosphine,450-490,515,BAO 9 (Bisaminophenyloxadiazole),365,395,BCECF (high pH),492, 503,520,528,BCECF (low pH),482,520,Berberine Sulphate,430,550,Beta Lactamase,409,447, 520,BFP blue shifted GFP (Y66H),381, 382, 383,445, 447, 448,blue shift

9、ed GFP (Y66H)Blue Fluorescent Protein,Blue Fluorescent ProteinBFP/GFP FRET,Bimane,398,490,Bisbenzamide,360,461,Bisbenzimide (Hoechst),360,461,bis-BTC = Ratio Dye, Zn2+,455/405,529/505,Ratio Dye, Zn2+Blancophor FFG,390,470,Blancophor SV,370,435,BOBO -1,462,481,BOBO -3,570,602,Bodipy 492/515,490,515,B

10、odipy 493/503,533,549,Bodipy 500/510,509,515,Bodipy 505/515,502,510,Bodipy 530/550,528,547,Bodipy 542/563,543,563,Bodipy 558/568,558,569,Bodipy 564/570,564,570,Bodipy 576/589,579,590,Bodipy 581/591,584,592,Bodipy 630/650-X,625,642,Bodipy 650/665-X,647,665,Bodipy 665/676,605,676,Bodipy Fl,504, 505,51

11、1, 513,Bodipy FL ATP,505,514,Bodipy Fl-Ceramide,505,511,Bodipy R6G SE,528,547,Bodipy TMR,542,574,Bodipy TMR-X conjugate,544,573,Bodipy TMR-X, SE,544,570,Bodipy TR,589,617,Bodipy TR ATP,591,620,Bodipy TR-X SE,588,616,BO-PRO -1,462,481,BO-PRO -3,544,570,Brilliant Sulphoflavin FF,430,520,BTC - Ratio Dy

12、e Ca2+,464/401,533/529,Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+,459/417,517/532,Ratio Dye, Zn2+Calcein,494,517,Calcein Blue,373,440,Calcium Crimson,588, 589,611, 615,Calcium Green,501, 506,531,Calcium Green-1 Ca2+ Dye,506,531,Ca2+ DyeCalcium Green-2 Ca2+,506/503,536,Ca2+Calcium Green-5N Ca2+,506,532,Ca

13、2+Calcium Green-C18 Ca2+,509,530,Ca2+Calcium Orange,549,575,576,Calcofluor White,385, 395, 405,437, 440, 445,Carboxy-X-rhodamine (5-ROX),576,601,Cascade Blue,377,420,398,423,399,Cascade Yellow,399,550,400,552,Catecholamine,410,470,CCF2 (GeneBlazer),CFDA,494,520,CFP - Cyan Fluorescent Protein,430, 43

14、3, 436, (453),474, 475, 476, (501),Cyan Fluorescent ProteinCFP/YFP FRET,Chlorophyll,480,650,Chromomycin A,436-460,470,Chromomycin A,445,575,CL-NERF (Ratio Dye, pH),504/514,540,Ratio Dye, pHCMFDA,494,520,Coelenterazine Ca2+ Dye, bioluminescence,-429,465,Ca2+ Dye, bioluminescence,native moleculeCoelen

15、terazine cp (Ca2+ Dye,),-430,442,Ca2+ Dye, bioluminescenceCoelenterazine f,-437,473,Ca2+ Dye, bioluminescenceCoelenterazine fcp,452,Ca2+ Dye, bioluminescenceCoelenterazine h,-437,464,Ca2+ Dye, bioluminescenceCoelenterazine hcp,-433,444,Ca2+ Dye, bioluminescenceCoelenterazine ip,441,Ca2+ Dye, biolumi

16、nescenceCoelenterazine n,-431,467,Ca2+ Dye, bioluminescenceCoelenterazine O,460,575,Coumarin Phalloidin,387,470,C-phycocyanine,CPM Methylcoumarin,384,469,MethylcoumarinCTC,400-450,602,CTC Formazan,Cy2,489,506,Cy3.1 8,554,568,Cy3.5,581,598,Cy3,514,566,552,570,554,Cy5.1 8,649,666,Cy5.5,675,695,Cy5,649

17、,666,670,Cy7,710, 743,767, 805,Cyan GFP,433 (453),475 (501),cyclic AMP Fluorosensor (FiCRhR),500,517,CyQuant Cell Proliferation Assay,480,520,Cell Proliferation AssayDabcyl,453,Dansyl,340,578,Dansyl Amine,337,517,Dansyl Cadaverine,335,518,Dansyl Chloride,372,518,Dansyl DHPE,336,517,Dansyl fluoride,3

18、56,none,DAPI,359,461,Dapoxyl,403,580,Dapoxyl 2,374,574,Dapoxyl 3,373,574,DCFDA,504,529,DCFH (Dichlorodihydrofluorescein Diacetate),505,535,DDAO,463,607,DHR (Dihydorhodamine 123),505,534,Di-4-ANEPPS,496,705,Di-8-ANEPPS (non-ratio),488,605,498,713,DiA (4-Di-16-ASP),456,591,Dichlorodihydrofluorescein D

19、iacetate (DCFH),505,535,DiD - Lipophilic Tracer,644,665,Lipophilic TracerDiD (DiIC18(5),644,665,DIDS,341,415,Dihydorhodamine 123 (DHR),505,535,DiI (DiIC18(3),549, 551,565,Dinitrophenol,349,DiO (DiOC18(3),484, 487,501, 502,DiR,748,780,Lipophilic TracerDiR (DiIC18(7),750,779,DM-NERF (high pH),497/510,

20、540,Ratio Dye, pHDNP,349,Dopamine,340,490-520,DsRed,558,583,Red fluorescent proteinDTAF,494,520,DY-630-NHS,621,660,Hemicyane,label for proteins and DNADY-635-NHS,634,664,Hemicyane,label for proteins and DNAEBFP,383,447,Enhanced Blue Fluorescent ProteinECFP,436,474,Enhanced Cyan Fluorescent ProteinEG

21、FP,488, 498,507, 516,Enhanced Green Fluorescent ProteinELF 97,345,530,Eosin,524,545,Erythrosin,529, 532,554, 555,Erythrosin ITC,529,555,Ethidium Bromide,510, 523,595, 605,Ethidium homodimer -1 (EthD-1),528,617,Euchrysin,430,540,EukoLight,Europium (III) chloride,EYFP,513, 520,527, 532,Enhanced Yellow

22、 Fluorescent ProteinFast Blue,360,440,FDA,494,520,Feulgen (Pararosaniline),570,625,FIF (Formaldehyd Induced Fluorescence),405,433,FITC,490, 494,520, 525,FITC Antibody,493,517,Flazo Orange,375-530,612,Fluo-3,480 506, 506,520, 527,Fluo-4,494,516,Fluorescein (FITC),490, 494,520, 525,Fluorescein Diaceta

23、te,494,520,Fluoro-Emerald,495,524,Fluoro-Gold (Hydroxystilbamidine),361,536,Fluor-Ruby,555,582,FluorX,494,520,FM 1-43,479,598,FM 4-46,515,640,Fura Red (high pH),572,657,Fura Red/Fluo-3,Fura-2, high calcium,335,505,Excitation ratio dyeFura-2, low calcium,363,512,Excitation ratio dyeFura-2/BCECF,Genac

24、ryl Brilliant Red B,520,590,Genacryl Brilliant Yellow 10GF,430,485,Genacryl Pink 3G,470,583,Genacryl Yellow 5GF,430,475,GeneBlazer (CCF2),GFP (S65T),498,516,GFP red shifted (rsGFP),498,516,GFP wild type, non-UV excitation (wtGFP),475,509,GFP wild type, UV excitation (wtGFP),395,509,GFPuv,385,508,Glo

25、xalic Acid,405,460,Granular Blue,355,425,Haematoporphyrin,530-560,580,Hoechst 33258,345,487,Hoechst 33342,347,483,Hoechst 34580,392,440,HPTS,355,465,Hydroxycoumarin,325-360,386-455,Hydroxystilbamidine (FluoroGold),361,536,Hydroxytryptamine,400,530,Indo-1, high calcium,330,401,Emission ratio dyeIndo-

26、1, low calcium,346,475,Emission ratio dyeIndodicarbocyanine (DiD),644,665,Indotricarbocyanine (DiR),748,780,Intrawhite Cf,360,430,JC-1,514,529,JO-JO-1,530,545,JO-PRO-1,532,544,LaserPro,795,812,Laurodan,355,460,LDS 751 (DNA),543,712,LDS 751 (RNA),590,607,Leucophor PAF,370,430,Leucophor SF,380,465,Leu

27、cophor WS,395,465,Lissamine Rhodamine,572, 577,591, 592,Lissamine Rhodamine B,577,592,LIVE/DEAD Kit Animal Cells,494,517,for more details referCalcein/Ethidium homodimer,528,617,to LOLO-1,566,580,LO-PRO-1,568,581,Lucifer Yellow,425, 428,528, 536, 540,Lyso Tracker Blue,373,422,Lyso Tracker Blue-White

28、,466,536,Lyso Tracker Green,504, 534,511, 551,Lyso Tracker Red,490,516,Lyso Tracker Yellow,551,576,LysoSensor Blue,374,424,LysoSensor Green,442,505,LysoSensor Yellow/Blue,384,540,Mag Green,507,531,Magdala Red (Phloxin B),524,600,Mag-Fura Red,483/427,659/631,Ratio Dye, Mg2+Mag-Fura-2,369/329,508,Rati

29、o Dye Ca2+,369/330,511/491,Ratio Dye Mg2+Mag-Fura-5,369/330,505/500,Ratio Dye, Ca2+,369/332,505/482,Ratio Dye, Mg2+Mag-Indo-1,349/328,480/390,Ratio Dye, Ca2+,349/330,480/417,Ratio Dye, Mg2+Magnesium Green,506, 507,531,Magnesium Orange,550,575,Malachite Green,628,Marina Blue,362,459,Maxilon Brilliant

30、 Flavin 10 GFF,450,495,Maxilon Brilliant Flavin 8 GFF,460,495,Merocyanin,555,578,Methoxycoumarin,360,410,Mitotracker Green FM,490,516,Mitotracker Orange,551,576,Mitotracker Red,578,599,Mitramycin,450,470,Monobromobimane,398,490,Monobromobimane (mBBr-GSH),398,500,Monochlorobimane,380,461,MPS (Methyl

31、Green Pyronine Stilbene),364,395,NBD,466,539,NBD Amine,450,530,Nile Red,515-555, 559,590, 640,Nitrobenzoxadidole,465,510-650,Noradrenaline,340,490-520,Nuclear Fast Red,289-530,580,Nuclear Yellow,365,495,Nylosan Brilliant Iavin E8G,460,510,Oregon Green,503,522,Oregon Green 488-X,494,517,Oregon Green,

32、503,522,Oregon Green 488,490, 493,514, 520,Oregon Green 500,497,517,Oregon Green 514,506,526,Pacific Blue,405,455,Pararosaniline (Feulgen),570,625,PBFI,340/380,420,Excitation ratio dyePE-Cy5,488,670,PE-Cy7,488,755, 767,PerCP,488,675,PerCP-Cy5.5,488,710,PE-TexasRed Red 613,488,613,Phloxin B (Magdala

33、Red),524,600,Phorwite AR,360,430,Phorwite BKL,370,430,Phorwite Rev,380,430,Phorwite RPA,375,430,Phosphine 3R,465,565,PhotoResist,365,610,Phycoerythrin B PE,546-565,575,Phycoerythrin R PE,565,578,PKH26 (Sigma),551,567,PKH67,496,520,ChromaPMIA,341,376,Pontochrome Blue Black,535-553,605,POPO-1,433,457,

34、POPO-3,533,574,PO-PRO-1,435,455,PO-PRO-3,539,567,Primuline,410,550,Procion Yellow,470,600,Propidium Iodid (PI),(305), 536, 538,617,PyMPO,412, 415,561, 564, 570,Pyrene,360,387,Pyronine,410,540,Pyronine B,540-590,560-650,Pyrozal Brilliant Flavin 7GF,365,495,QSY 7,560,Quinacrine Mustard,440,510,Red 613

35、 PE-TexasRed,488,613,Resorufin,571,584, 585,RH 414,532,716,Rhod-2,552,576,Rhodamine,550,573,Rhodamine 110,496, 497,520,Rhodamine 123,507,529,Rhodamine 5 GLD,470,565,Rhodamine 6G,525,555,Rhodamine B,540,625,Rhodamine B 200,523-557,595,Rhodamine B extra,550,605,Rhodamine BB,540,580,Rhodamine BG,540,57

36、2,Rhodamine Green,502,527,Rhodamine Phallicidine,558, 542,575, 565,Rhodamine Phalloidine,542,565,Rhodamine Red,570,590,Rhodamine WT,530,555,Rose Bengal,525, 540,550-600,R-phycocyanine,R-phycoerythrin (PE),565,578,rsGFP,473,509,red shifted GFP (S65T)S65A,471,504,S65C,479,507,S65L,484,510,S65T,488,511

37、,Sapphire GFP,395,511,SBFI,340/380,420,Excitation ratio dyeSerotonin,365,520-540,Sevron Brilliant Red 2B,520,595,Sevron Brilliant Red 4G,500,583,Sevron Brilliant Red B,530,590,Sevron Orange,440,530,Sevron Yellow L,430,490,sgBFP,387,450,sgBFP (super glow BFP),387,450,Quantums SuperGloGFP,AFPssgGFP,47

38、4,488,sgGFP (super glow GFP),474,509,Quantums SuperGloGFP,AFPsSITS,336,436,Ion ChannelsSITS (Primuline),395-425,450,SITS (Stilbene Isothiosulphonic Acid),365,460,SNAFL calcein,506/535,535/620,Ratio Dye, pHSNAFL-1,508/540,543/623,Ratio Dye, pHSNAFL-2,514/543,546/630,Ratio Dye, pHSNARF calcein,552/574

39、,590/629,Ratio Dye, pHSNARF1,576/548,635/587,Excitation and emission ratio dyeSodium Green,506, 507,532,Na+, K+SpectrumAqua,433,/53,480/55,VysisSpectrumGreen,497/30, 509/31,538/44, 524/56,VysisSpectrumOrange,559/38, 560,588/48,VysisSpectrum Red,587, 587/35,612, 612/51,SPQ (6-methoxy-N-(3-sulfopropyl

40、)quinolinium),344,443,Stilbene,335,440,Sulphorhodamine B can C,520,595,Sulphorhodamine G Extra,470,570,SYTO 11,508, 510,527, 530,Dye for DNA, RNASYTO 12,499, 500,522, 519,Dye for DNA, RNASYTO 13,488, 491,509, 514,Dye for DNA, RNASYTO 14,517, 521,549, 547,Dye for DNA, RNASYTO 15,516, 518,546, 555,Dye

41、 for DNA, RNASYTO 16,488, 494,518, 525,Dye for DNA, RNASYTO 17,621,634,Dye for DNASYTO 18,490, 493,507, 527,Dye for DNA, RNASYTO 20,512,530,Dye for DNASYTO 21,494,517,Dye for DNASYTO 22,515,535,Dye for DNASYTO 23,499,520,Dye for DNASYTO 24,490,515,Dye for DNASYTO 25,521,556,Dye for DNASYTO 40,420,441,Dye for DNASYTO 41,430,454,Dye for DNASYTO 42,433,460,Dye for DNASYTO 43,436,467,Dye for DNASYTO 44,446,471,Dye for DNASYTO 45,452,484,Dye for DNASYTO 59,622,645,Dye for DNASYTO 60,652