有機磷農(nóng)藥檢測（英文）

PesticideresidueWhatisapesticide?Pesticidemeansanysubstanceintendedfor

preventing,destroying,attracting,repelling,orcontrollinganypestincludingunwantedspeciesofplantsoranimalsduringtheproduction,storage,transport,distribution,andprocessingoffood,agriculturalcommodities,oranimalfeedsorwhichmaybeadministeredtoanimalsforthecontrolofectoparasites.

Thetermincludessubstancesintendedforuseasaplant-growthregulator,defoliant,desiccant,fruitthinningagent,orsproutinginhibitorSubstancesappliedtocropseitherbeforeorafterharvesttoprotectthecommodityfromdeteriorationduringstorageandtransportAccordingtotheactiveelement,thepesticideincludeorganochlorinepesticideorganophosphorouspesticidecarbamatepesticidesyntheticpyrethroidpesticideothersthepesticidecanexistinAir:sprypesticideWater:whenwashthepesticidebottleinriverraincancarrythepesticideintothewaterItalsocanentertheundergroundwaterSoil:sprypesticideEnterthepeople’sbodybyRespiratorytract：breathAlimentarycanal:drinkoreatfoodSkinChronictoxicityLongtermeffectCancerBirthdefectsInducetumorsEndocrinedisruptingchemicalDoYouKnowAllvegetablesandfruitsaresprayedwithtoxicsyntheticinsecticides&fungicidesofwhichsomearecarcinogenic(cancercausing).Allvegetables&fruitsfromfarmtothemarketplacemaygothroughhighlyunhygienichandlingtilltheyfinallyreachyou.(seed)Sowninthefarmer’sfieldpre-harvesttreatmentwithtoxicinsecticidestreatedwithtoxicinsecticides/fungicidesharvestedtradedinmarketplaceconsumerhomewaterwashed&consumedWhatisapesticideresidueOneormoresubstancespresentinoronplantsorproductsofplantorigin,edibleanimalproductsorelsewhereintheenvironmentandresultingfromtheuseofpesticide,includingtheirmetabolitesandproductsresultingfromtheirdegradationorreactionWhenacropistreatedwithapesticide,averysmallamountofthepesticide,orindeedwhatitchangestointhe

plant

(its'metabolites'or'degradationproducts'),canremaininthecropuntilafteritisharvested.Thisisknownasthe'residue'.

Pesticide

residuesmaybepresentin:

freshortinnedfruitandvegetablesprocessedfoodanddrink

madefromthecrop(e.g.juice,breadoranyothermanufacturedfoodordrink)freshorprocessedanimalproducts(iftheanimalshavebeenfedoncroptreatedwithpesticide).Itisimportant:toinsurequalityofagricultureproductsandtopreventpesticidepollution.achievedbyintensifyinginspectionandmonitoringsystem.instantdetectiontechniquesforpesticideresidue.MeasurementofthepesticideresidueObjectiveToqualitativeanalysistheresidueoforganophosphorousorcarbamatepesticidesinvegetables

Method

Calculatetheacetylcholinesteraseinhibitionrate

PrincipiumTheorganophosphorousorcarbamatepesticidescouldinhibittheactivitiesoftheacetylcholinesteraseTherewasapositiverelationshipbetweentheacetylcholinesteraseinhibitionrateandthepesticideconcentrationCont…MeasurerangeVegetablesleaf,fruit,bean,melonandsoonPesticidesOrganophosphorous:forexample,methamidophos,parathion,omethoate,dichlorvos,phorate,etc.Carbamates:forexample,carbaryl,methomyl,metolcarb,etc.

InstrumentsSP-723PVis-spectrophotometercultureboxelectronicbalancebeakerSalver：tweezers,shears,knifeandpenciltesttubeandtubeshelfPlasticsqueezebottleRubberpipettebulbPipette–sample,extractionsolutionMicrosampler：substrate,developer,acetylcholinesteraseReagentsThereagentpackageincludeacetylcholinesterase,substrate,developerandextractionsolutionAllofthewaterisdistilledwaterAllofthereagentsareanalyticalreagentinthisexperimentCont…1.extractionreagent:(phosphatebuffersolution)

Na2HPO4+KH2PO4;PH=8.0Dissolveonebaginto1000mldistilledwater2.specialenzyme:(acetylcholinesterase)powder,freezepreservedeverybottledissolvedinto8mldistilledwaterCont…3.substrate：（DithioAcetylcholineIodide

）dissolvedinto10mlextractionsolutionpreservedinthetemperatureof0~5℃4.developer:（DisulfideDinitroBenzoicacid）dissolvedinto10mlextractionsolutionpreservedinthetemperatureof0~5℃.Cont…SamplingrequirementLeafusingthesamplingequipmenttotaketheleavesfromthedifferentplantsatrandomFruittakingasamplefromthesurfacethicknessis1cm3mlextractionsolution

+50μlacetylcholinesterase,+50μldeveloper,culturingfor30minproductions+50μlsubstrate

Shakeupspilltocuvettemeasureimmediatelyyellowsolutiondifferenceofabsorbencyvalueoffore-and-aft3min.spectrophotometerActhecontrol3mlfiltratesolution+50μlacetylcholinesterase,+50μldeveloper,culturingfor30minproductions+50μlsubstrate

Shakeupspilltocuvettemeasureimmediatelyyellowsolutiondifferenceofabsorbencyvalueoffore-and-aft3min.spectrophotometerAsVegetablesample+20mlextractionsolutionandthenfiltratetheinhibatonrate>50%?Pesticideresidue?thesampleSampletreatment

inhibitoryreaction(add50μlacetylcholinesterase,3mlfiltrateand50μldevelopertothetesttube)Sampling(take2gramsample,or4gramifitisnotleafvegetable,andthencutup)Extracting(add20mlextractionsolution,shakefor1or2min,filtrate,andthenplacefor3min.)Culturing(putthesampletothecultureboxof37-38Cfor30min.)Color

reaction(takethesampleoutoftheculturebox,add50μlsubstrate,filltothecuvetteandmeasureon723Pspectrophotometerimmediately)

add50μlacetylcholinesterase,3mlextractionsolutionand50μldevelopertotesttubeCulturingputthecontroltothecultureboxof37-38Cfor30min.Color

reactiontakethesampleoutoftheculturebox,add50μlsubstrate,filltocuvetteandthenmeasureon723PspectrophotometerimmediatelyControl-sampletreatmentCalculatetheinhibitionrate

X:theinhibitionrateAc:thecontrol’sdifferenceofabsorbencyvalueoffore-and-aft3minAs:thesample’sdifferenceofabsorbencyvalueoffore-and-aft3minAnalysistheresulttheresultisexpressedasinhibitionrate(%)therate＜50％,free-pesticideresiduetherate≥50％,pesticideresidueexcessiveAttentionAc:0.4~0.8；ifthevalueofAcislessthan0.4,addmoreacetylcholinesterasepropriety.Afteraddingthesubstrate,colorimetricanalysisatonce.Themicrosampleroftheenzyme,chromogenicreagentandthesubstratewasforbiddentobeusedinconfusion.Cont….SP-732P

VisSpectrophotometerprinterparameterdisplayoperationpanelsampleroomswitchbetweenprinterandcomputer①.

DIGITALREADOUT.displaysthedataofTransmittance,Absorbance,Concentration.②③.WAVELENGTHSELECTOTKEYSis

usedtoselectthedesiredanalyticalwavelength,andusedtoselectthespecialfunctionsassettingtheparameterofstandardconcentration(concentrationvalueorfactor),lightsourceexchangeselection,LampON/OFF,etc.④FUNCTIONSELECTORselectsthefunctionsassettingtheparameterofstandardconcentration(concentrationvalueorfactor),lightsourceexchangeselection,LampON/OFF,etc.⑤ENTERKEYisusedtoenteringtheparameterofsampletothememoryorconfirmingthefunctionthatisselected.⑥100%T/0ACONTROLisusedtosetthedisplayto100%Tor0Abs.Itmustberesetwhenevertheanalyticalwavelengthhasbeenchanged.⑦MODESELECTORselectstheTransmittance,Absorbance,Concentrationmode.（tosetthemodeoftesting,choosethefirstmodewhichcanmeasure8samplesatthesametime,orthesecondmodewhichcanmeasure9samplesatthesametime.）⑧0%TCONTROL

isusedtosetthedisplayto0%Tbeforesetting0Abs.⑨CLEANKEYisusedforcleaningthewrongparameter.Sketchmapofthesamplecompartmentsamplesamplesamplesamplesamplesamplesamplesamplecontroladjust100%T12345678910operatingprocedure1.TurntheinstrumentonClosethesamplecompartmentcover,andturnonthepowerAllowtheinstrumenttoself-correctingandwarmupforatleast30minutes2.whentheself-correctingfinish,theinstrumententerthefollowinginterface3.pressthefunctionselectorkeytochoosethefunctionoffastmeasurementofpesticideresidue

功能

請輸入標準濃度

請輸入標準因子

請輸入波長切換點

建立標準曲線。

農(nóng)藥殘毒快速檢測

調(diào)用標準曲線

刪除標準曲線functionpleaseinputthestandardconcentrationpleaseinputthestandardfactorspleaseinputthepointofwavelengthswitchdrawthestandardcurvefastmeasurementofthepesticideresiduetransferthestandardcurvedeletethestandardcurve農(nóng)藥殘毒快速檢測請放入標樣及被測樣品開始檢測？正在檢測……

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