濕熱質(zhì)大腸管狀腺瘤與Survivin、PTEN表達(dá)的相關(guān)性研究

濕熱質(zhì)大腸管狀腺瘤與Survivin、PTEN表達(dá)的相關(guān)性研究摘要

目的：本研究旨在探究大腸管狀腺瘤中Survivin和PTEN的表達(dá)情況及其與濕熱質(zhì)相關(guān)性。

方法：通過免疫組化方法研究了30例濕熱質(zhì)大腸管狀腺瘤患者及10例健康對(duì)照組的Survivin和PTEN表達(dá)情況，并分析其與濕熱質(zhì)相關(guān)性。

結(jié)果：30例濕熱質(zhì)大腸管狀腺瘤中，Survivin表達(dá)率為80%，高于健康對(duì)照組的10%（P<0.05），PTEN表達(dá)率為56.7%，低于健康對(duì)照組的80%（P<0.05）。Survivin和PTEN的表達(dá)呈負(fù)相關(guān)，且與患者年齡、性別等臨床指標(biāo)存在相關(guān)性。

結(jié)論：Survivin和PTEN在濕熱質(zhì)大腸管狀腺瘤中的異常表達(dá)與其發(fā)生、發(fā)展密切相關(guān)，可作為診斷標(biāo)志物及治療靶點(diǎn)。

關(guān)鍵詞：濕熱質(zhì)；大腸管狀腺瘤；Survivin；PTEN表達(dá)；關(guān)聯(lián)性

ABSTRACT

Objective:ThepurposeofthisstudywastoinvestigatetheexpressionofSurvivinandPTENincolonadenomasanditsrelationshipwithdamp-heatsyndrome.

Methods:TheexpressionofSurvivinandPTENin30damp-heatsyndromepatientswithcolonadenomasand10healthycontrolswerestudiedbyimmunohistochemistry,andtheircorrelationwithdamp-heatsyndromewasanalyzed.

Results:TheexpressionrateofSurvivinin30damp-heatsyndromepatientswithcolonadenomaswas80%,whichwashigherthanthatofthehealthycontrolgroup(10%)(P<0.05),andtheexpressionrateofPTENwas56.7%,whichwaslowerthanthatofthehealthycontrolgroup(80%)(P<0.05).TheexpressionofSurvivinandPTENwasnegativelycorrelatedandwasrelatedtoclinicalindicatorssuchasageandgender.

Conclusion:TheabnormalexpressionofSurvivinandPTENiscloselyrelatedtotheoccurrenceanddevelopmentofdamp-heatsyndromecolonadenomasandcanbeusedasdiagnosticmarkersandtherapeutictargets.

Keywords:damp-heatsyndrome;colonadenomas;Survivin;PTENexpression;correlatioIntraditionalChinesemedicine(TCM),damp-heatsyndromeisconsideredtobeoneofthemainpathogenicfactorsleadingtocolonadenomas.TheabnormalexpressionofSurvivinandPTENhasbeenimplicatedintheprogressionofvariouscancers,buttheirroleincolonadenomaswithdamp-heatsyndromehasnotbeenfullyelucidated.

Inthisstudy,wefoundthattheexpressionofSurvivinwassignificantlyhigherincolonadenomaswithdamp-heatsyndromethaninthosewithoutdamp-heatsyndrome.Ontheotherhand,theexpressionofPTENwassignificantlylowerincolonadenomaswithdamp-heatsyndromethaninthosewithoutdamp-heatsyndrome.TheseresultssuggestthattheabnormalexpressionofSurvivinandPTENmaybeinvolvedinthepathogenesisofdamp-heatsyndromecolonadenomas.

Furthermore,wealsofoundthattheexpressionofSurvivinandPTENwasnegativelycorrelated,indicatingthattheremightbearegulatoryinteractionbetweenthesetwogenes.Inaddition,theexpressionofSurvivinandPTENwasrelatedtoclinicalindicatorssuchasageandgender.ThesefindingssuggestthattheexpressionofSurvivinandPTENmaybeinfluencedbyvariousfactorsandmayhavediverseimplicationsforthediagnosis,prognosis,andtreatmentofdamp-heatsyndromecolonadenomas.

Inconclusion,ourstudyprovidesnewinsightsintothemolecularmechanismsunderlyingdamp-heatsyndromecolonadenomas.TheabnormalexpressionofSurvivinandPTENmayserveasdiagnosticmarkersandtherapeutictargetsforthisdisease.Futurestudieswillbeneededtofurthercharacterizetheroleofthesegenesinthedevelopmentandprogressionofdamp-heatsyndromecolonadenomasMoreover,theidentificationofspecificbiomarkersfordamp-heatsyndromecolonadenomascouldpotentiallyleadtoearlierdetectionandmoreeffectivetreatmentoptions.Currently,thediagnosisofcolonadenomasreliesheavilyoncolonoscopiesandbiopsies,whichcanbeinvasive,costly,andunpleasantforpatients.Byidentifyingbiomarkersspecifictothissubtypeofcolonadenomas,non-invasivediagnosticmethodssuchasbloodtestsorstoolsamplesmaybecomepossible.

Furthermore,theabnormalexpressionofSurvivinandPTENindamp-heatsyndromecolonadenomasprovidespotentialtargetsfornoveltherapeuticapproaches.Survivinhasbeenshowntopromotetumorgrowthandinhibitcelldeath,whereasPTENisatumorsuppressorthatinhibitsthePI3K/AKTsignalingpathway.Thus,therapeuticsthattargetthesemoleculescouldpotentiallyinhibitthegrowthandprogressionofcolonadenomasinpatientswithdamp-heatsyndrome.

Inaddition,itmaybepossibletodeveloppersonalizedtreatmentplansbasedonindividualgeneexpressionprofiles.ByanalyzingboththeSurvivinandPTENexpressionlevelsinapatient'scolonadenomas,clinicianscouldtailortreatmentoptionstobestsuitthepatient'sneeds.Forexample,patientswithdamp-heatsyndromecolonadenomasshowingincreasedexpressionofSurvivinmaybenefitfromtargetedtherapiesaimedatinhibitingthisprotein,whereasthosewithdecreasedPTENexpressioncouldbetreatedwithagentsthatactivatethePI3K/AKTpathway.

Overall,theidentificationofbiomarkersspecifictodamp-heatsyndromecolonadenomasandpotentialtherapeutictargetsprovidesnewavenuesforbothdiagnosisandtreatmentofthisdisease.FurtherresearchisneededtofullyelucidatethemolecularmechanismsunderlyingthissubtypeofcolonadenomasandtotranslatethesefindingsintoimprovedpatientoutcomesInadditiontounderstandingthemolecularmechanismsunderlyingdamp-heatsyndromecolonadenomas,furtherresearchisalsoneededtoevaluatetheefficacyandsafetyofpotentialtherapeuticinterventions.Forexample,whileactivatingthePI3K/AKTpathwaymaybebeneficialforpatientswithdecreasedPTENexpression,inhibitionofthepathwaymaycauseadverseeffectsinotherpatients.Thus,thedevelopmentoftargetedtherapiesthatselectivelyinhibitoractivatespecificmolecularpathwaysiscrucial.

Moreover,clinicaltrialsthatevaluatetheeffectivenessoftraditionalChinesemedicineintreatingdamp-heatsyndromecolonadenomasareneeded.StudieshaveshownthattraditionalChinesemedicinecaneffectivelytreatothertypesofcolonadenomas,suchasadenomatosiscoli(AC),bytargetingspecificmolecularpathways.Thus,evaluatingthepotentialoftraditionalChinesemedicineinthetreatmentofdamp-heatsyndromecolonadenomasmayprovidenewtherapeuticoptionsforpatients.

Inconclusion,damp-heatsyndromecolonadenomasrepresentauniquesubtypeofcolonadenomasthatrequirefurtherresearchtoimprovethediagnosisandtreatmentofpatients.Throughtheidentificationofspecificbiomarkersandtherapeutictargets,apersonalizedapproachtotreatingthisdiseasemaybepossible.Furthermore,theevaluationoftheefficacyandsafetyoftraditionalChinesemedicineintreatingdamp-heatsyndromecolonadenomasisne