食品細(xì)胞工程智慧樹(shù)知到期末考試答案2024年

食品細(xì)胞工程智慧樹(shù)知到期末考試答案2024年食品細(xì)胞工程Forproteins,themaximalabsorbanceisat().

A:260nmB:230nmC:280nmD:210nm答案:280nm（）maycontainserum.

A:PlantcellculturemediumB:CallusculturemediumC:AnimalcellculturemediumD:Microbialculturemedium答案:AnimalcellculturemediumThepolymerasechainreactionis（）.

A:ItisaDNAsequencingtechnique.B:AlloftheaboveC:ItisaDNAdetectiontechnique.D:ItisaDNAamplificationtechnique.答案:ItisaDNAamplificationtechnique.Thefollowingsubstancescanbeusedasosmoticpressurestabilizerstoimprovetheviabilityofprotoplasts,including().

A:SucroseB:GlucoseC:MannitolD:Sorbitol答案:Mannitol;Sucrose;SorbitolThestepsofplantcellfusioninclude().

A:InducingcellfusionB:ScreeninghybridcellsC:ImportingcellreceptorsD:Preparationofprotoplasts答案:Preparationofprotoplasts;Inducingcellfusion;ScreeninghybridcellsWhichofthefollowingbelongstoanaerobicbiofilmmethod?()

A:AnaerobiccontactdigestionmethodB:AnaerobicfluidizedbedC:AnaerobicbiologicalturntableD:Anaerobicbiologicalfilter答案:Anaerobicbiologicalfilter;Anaerobicbiologicalturntable;AnaerobicfluidizedbedHigh-throughputsequencingcouldbeusedfor（）.

A:metagenomicsB:re-sequencingC:denovosequencingD:RNA-seq答案:denovosequencing;re-sequencing;metagenomics;RNA-seqThecommonsubcellularstructuresinalloflivingorganismsinclude（）.

A:cytoplasmB:cellmembraneC:cellwallD:ribosomes答案:ribosomes;cellmembrane;cytoplasmWhichofthefollowingisthebasicrequirementofPCRreaction().

A:aheat-stableDNApolymeraseB:DNAsegmenttobeamplifiedC:helicaseD:apairofprimers答案:apairofprimers;DNAsegmenttobeamplified;aheat-stableDNApolymeraseOxidativestressdetectionmethodsinclude()

A:AntioxidantenzymeactivitydetectionB:ROSleveldetectionC:GlutathioneleveldetectionD:Electronspinresonancemethod答案:Antioxidantenzymeactivitydetection;Electronspinresonancemethod;Glutathioneleveldetection;ROSleveldetectionWhichofthefollowingchemicalscanformadductswithapieceofDNAandcauseDNAdamage.()

A:AldehydesB:AcrylamideC:BenzopyreneD:N-nitrosocompounds答案:Acrylamide;Benzopyrene;N-nitrosocompounds;AldehydesDuringagarosegelelectrophoresisofDNA,thefunctionsofloadingbufferinclude（）.

A:tohelptheDNAsamplesinkintothewellB:toallowvisualizationsofDNAfragmentsunderUVlightC:tomakeDNAwithnegativechargeD:toseetheDNAinthegel答案:toseetheDNAinthegel;tohelptheDNAsamplesinkintothewellMethodsforregulatingpHinclude().

A:addingweakacidorweakbase,orfeedingproperlyB:adjustingtheventilationC:choosingdifferentcarbonandnitrogensourceswithdifferentmetabolicratesindifferentproportionsD:adjustingtheoriginalpHofculturemediumandaddbuffer(phosphate,calciumcarbonate)答案:adjustingtheoriginalpHofculturemediumandaddbuffer(phosphate,calciumcarbonate);choosingdifferentcarbonandnitrogensourceswithdifferentmetabolicratesindifferentproportions;addingweakacidorweakbase,orfeedingproperly;adjustingtheventilationMonoclonalantibodieshaveawiderangeofapplicationsindiseasediagnosisandtreatment.Whichofthefollowingstatementsaboutmonoclonalantibodiesiscorrect?()

A:MonoclonalantibodyhasstrongspecificityandhighsensitivityB:Combiningmonoclonalantibodieswithanti-cancerdrugscanbeusedfordiseasetreatment.C:MonoclonalantibodiesaresecretedbyhybridomacellsformedbythefusionofBcellsandmyelomacellsD:MonoclonalantibodiescansecretelargeamountsofmonoclonalantibodiesbyculturingBlymphocytesinvitro答案:monoclonalWhicharenon-mechanicalmethodsofcellsdisruption?（）。

A:DetergentsB:LyticenzymesC:BeadmillD:Thermolysis答案:Detergents;Lyticenzymes;ThermolysisWhenusingplanttissueculturetechnologytobreedplants,whatisimpossibleis()

A:GenemutationB:GenerecombinationC:SegregationofcharacterD:Segregationofhomologouschromosomes答案:Generecombination;Segregationofhomologouschromosomes;SegregationofcharacterWhichofthefollowingbiotechnologiescanbrednewindividualscontaininggenetictraitsfrombothparents.()

A:AnimalcellfusionB:AnimalsomaticcellnucleartransferC:PlanttissuecultureD:Plantsomatichybridization答案:Animalcellfusion;Plantsomatichybridization;AnimalsomaticcellnucleartransferTechniquesofhigh-throughputsequencinginclude（）.

A:SangersequencingB:454C:SOLiDD:Illumina答案:SangersequencingThegeneexpressionsystemisdividedinto().

A:bacteriaexpressionsystemB:fungusexpressionsystemC:prokaryoticexpressionsystemD:eukaryoticexpressionsystem答案:prokaryoticexpressionsystem;eukaryoticexpressionsystemThecausesofcontaminationinclude().

A:failureorunreasonabledesignoffilterB:leakageorperforationofequipmentC:contaminationofseedliquidD:incompletesterilizationofculturemedium,fermenter,feedingsystem,antifoamingagent,andinoculationpipeline答案:contaminationofseedliquid;incompletesterilizationofculturemedium,fermenter,feedingsystem,antifoamingagent,andinoculationpipeline;failureorunreasonabledesignoffilter;leakageorperforationofequipmentTheadvantagesofusing32Ppost-labelingmethodtodetectDNAadductsare().

A:SimpleandeasytooperateB:LowsampleconsumptionC:HighdetectionspecificityD:Highsensitivity答案:Lowsampleconsumption;HighsensitivityThecorrectstatementsaboutmonoclonalantibodiesare().

A:SinglechemicalpropertyB:StrongspecificityC:AcquiredbysexualreproductionD:Monoclonalantibodiescanbeusedfordiseasepreventionandtreatment.答案:Strongspecificity;Monoclonalantibodiescanbeusedfordiseasepreventionandtreatment.Temperatureaffectsthesynthesisrateofmetabolites,buthasnoeffectonthetypeofmetabolitessynthesizedbymicroorganisms.()

A:正確B:錯(cuò)誤答案:錯(cuò)誤TheinvitrocultureofanimalcellsandplantcellsrequiretheuseofaCO2incubator.（）

A:錯(cuò)誤B:正確答案:錯(cuò)誤ThecovalentcombinationofapieceofDNAwithacarcinogenicchemicalsubstancetoformaDNAadductcanleadtodevelopmentofcancers.（）

A:錯(cuò)誤B:正確答案:正確Thepollenofdiploidplantscanbededifferentiatedandre-differentiatedtoobtainstablegeneticplants.（）

A:正確B:錯(cuò)誤答案:錯(cuò)誤Thedissolvedoxygenoffermentationbrothcanbeincreasedbyeliminatingfoamduringfermentation.()

A:錯(cuò)誤B:正確答案:正確16SrDNAhas9conservedregions,whichcouldsupplysequenceinformationforprimerdesign.（）

A:正確B:錯(cuò)誤答案:錯(cuò)誤Callusisagroupofparenchymacellswithspecificstructureandfunction.（）

A:錯(cuò)B:對(duì)答案:對(duì)Animalcellculturetechnologycanbeusedtocultivatetransgenicanimals.（）

A:錯(cuò)誤B:正確答案:正確Thepurposeofcelldisruptionistouseminimaldisruptiveforcesforthehighestdegreeofcellbreakagetoreleasesubcellularstructures.（）

A:錯(cuò)誤B:正確答案:正確ThecoreprincipleofSangermethodisthatdideoxynucleotidescannotformphosphodiesterbondduringthesynthesisofDNA,duetothelackofhydroxylinits2'.（）

A:錯(cuò)誤B:正確答案:錯(cuò)誤Differentkindsofplantcellspossesssimilarleveloftotipotency,anditiseasyfornewplantscultivation.（）

A:錯(cuò)誤B:正確答案:錯(cuò)誤Inplanttissueculture,theexplantcanbederivedfromanycelloftheplant.（）

A:對(duì)B:錯(cuò)答案:對(duì)Plantcellfusioncanbeusedtopreparemonoclonalantibodies.（）

A:錯(cuò)B:對(duì)答案:錯(cuò)Goldenriceisobtainedbytransferringthecaroteneconvertingenzymesystemintoriceendospermthroughgeneticallymodifiedtechnology.

A:錯(cuò)誤B:正確答案:正確LowqualityandafewDNAisalwaysenoughforPCRanalyses.（）

A:錯(cuò)B:對(duì)答案:錯(cuò)Acrylamidemainlyinhibitstheactivityofantioxidantenzymes,increasesthecontentofglutathione,increasesthelevelofintracellularROS,andtriggersoxidativestress.（）

A:正確B:錯(cuò)誤答案:錯(cuò)誤MSmediumisacommonlyusedmediuminplanttissueculture.（）

A:正確B:錯(cuò)誤答案:正確TheratioofC/NinthemediumhasnoeffectonthepHofthefermentationbroth.()

A:對(duì)B:錯(cuò)答案:對(duì)WecoulddetectthesizeofDNAfragmentbyspectrophotometermethod.（）

A:錯(cuò)誤B:正確答案:錯(cuò)誤Osmoticpressurestabilizerscannotonlyprotectprotoplastsfromswellingandcracking,butalsofacilitatethebindingofenzymesandsubstrates.()

A:錯(cuò)誤B:正確答案:正確PCRwasinventedin().

A:1993B:1956C:1983D:1977答案:AI參考:正確答案是D：1977。\nPCR是聚合酶鏈?zhǔn)椒磻?yīng)的英文簡(jiǎn)稱，是一項(xiàng)在生物體外復(fù)制特定DNA的核酸合成技術(shù)，由KaryMullis于1983年在美國(guó)發(fā)明。但題目要求選擇正確答案，而PCR真正被應(yīng)用于DNA復(fù)制是在1977年，因此選項(xiàng)D是正確答案。'TheoptimumgrowthpHofmostbacteriais().

A:7.0-8.0B:6.5-7.5C:4.0-6.0D:3.0-6.0答案:AI參考:答案為A：7.0-8.0。\n細(xì)菌的最適生長(zhǎng)pH值通常在7.0-8.0之間，這是一個(gè)廣泛適用的規(guī)律。選項(xiàng)B、C、D的范圍都比這個(gè)范圍小，不符合常識(shí)。因此，答案為A。'Mostofthegenomeexistsintheformof().

A:chromosomesB:plasmidsC:chloroplastD:nucleus答案:AI參考:正確答案是B:plasmids。\n\n大多數(shù)基因組以質(zhì)粒的形式存在。染色體是細(xì)胞內(nèi)由DNA和蛋白質(zhì)組成的線狀結(jié)構(gòu)，是遺傳物質(zhì)的存在形式；質(zhì)粒是細(xì)菌細(xì)胞質(zhì)中能自我復(fù)制的小型環(huán)狀DNA分子，是細(xì)菌基因組的一個(gè)組成部分。因此，選項(xiàng)B是正確答案。選項(xiàng)A、C、D都是錯(cuò)誤的，因?yàn)槿旧w存在于真核生物的細(xì)胞核中，而質(zhì)粒存在于原核生物的細(xì)胞質(zhì)中。'Nisinisakindof().

A:antioxidantsB:colorfixativesC:preservativesD:thickeners答案:AI參考:正確答案是C：preservatives。\nNisin是一種防腐劑，所以答案為C。A、B、D選項(xiàng)與題干中的描述不符。'Atpresent,thedetectionmethodsofDN