人類皰疹病毒精美生物醫(yī)學

人類皰疹病毒Containsseveralofthemostimportanthumanpathogens01Aspectrumofdiseases02Lifelongpersistentinfection03Periodicreactivation04Virion:spherical,150-200nmcore+capsid+envelopedsDNA,encodesatleast100proteins35proteinsstructureofvirusReplication:InthenucleusBudfromnuclearmembraneEarlyreplicationandlatereplication010203040506CPE:apartfromEBV,otherscancauseCPEincellcultureFormofinfectionReplicationinfection:primary,reactivatedLatentinfectionCongenitalinfection(先天性感染)Celltransformation病毒infectedcellsdiseasesHSV1粘膜上皮細胞／感覺神經(jīng)節(jié)細胞多種疾病及潛伏感染HSV2粘膜上皮細胞／感覺神經(jīng)節(jié)細胞生殖器皰疹及潛伏感染VZV皮膚細胞／脊髓后根神經(jīng)節(jié)，顱神經(jīng)的感覺神經(jīng)節(jié)水痘／帶狀皰疹EBVB細胞，上皮細胞／B細胞傳染性單核細胞增多癥Burkitt瘤，鼻咽癌CMV上皮細胞，白細胞，精子細胞／分泌腺體多種疾病HHV6CD4+T細胞嬰兒丘疹（唾液傳播）免疫低下：持續(xù)急性感染HHV7CD4+T細胞幼兒皮疹（唾液傳播）與其他疾病關系不清HHV8不清Kaposi肉瘤有關PropertiesTwoserotypes:HSV1andHSV2HSVgenome:encodesatleast70proteinssomeassociatedwithviralreplicationsomeenvelopeproteinsassociatedwithimmunereactionviralpenetrationHerpessimplexvirus（HSV）PathogenicitySourceofinfectionpatientsandcarriersRoutesoftransmissionHSV1：

contactHSV2：

sexually/maternalgenitalinfectionPathology:cytolyticinfection

Primaryinfectionmostareinapparent,butresultinantibodyproductionandlatentinfection010203040506HSV1Gingivitis(齒齦炎):mostfrequentlyKeratoconjunctivitis(角膜結膜炎)SkininfectionEncephalitisLatentinfection:inthetrigeminalganglia(三叉神經(jīng)節(jié))HSV2Genitalherpes:commonseenSkinherps:asHSV1Neonatalherpes:duringbirth,isaseverediseaseskin,eye,monthlesionencephalitismultipleorgandisseminated(多器官感染)Latentinfection：sacralganglia(骶神經(jīng)節(jié))Latentandrecurrenceinfection01virusreactivate,replication03peripheralsites,skinormucousmembrane05HSV1,HSV2neuron02axon(神經(jīng)纖維)04HSV1：口唇部皰疹HSV2：genitalherpes06similarasHSVVZVnakedVZVenvelopedPathogenicitySourceofinfection：patientsRoutesofinfection:respiratorytractDisease：

水痘(Varicella/chickenpox)：兒童：斑丘疹，水皰，偶見并發(fā)癥孕婦：癥狀較重，可致胎兒畸形，流產，死胎免疫低下：重癥水痘

Latentinfectiondorsalrootganglia(脊髓后根神經(jīng)節(jié))Zoster(帶狀皰疹)SeeninadultsSeverepainandvesicle,ofteninchest020103

Cytomegalovirus(CMV)

巨細胞病毒Thelargest

geneticcontentofHHVVeryspeciesspecificandcellspecific(種屬和細胞特異性)CharacteristicCPE:

multinucleatedcellsandintranuclearinclusionIsspreadcelltocellPathogenicitySourceofinfection:patients/inapparentpatientsRoutesofinfectionClosecontactMothertochildDiseasesNormalhostMostaresubclinicalMononucleosislikesyndrome(單核細胞增多癥)amildillnessLatentinfectionImmunosuppressedhostMuchmoresevereDisseminatedpneumoniaInAIDS:胃腸炎，視網(wǎng)膜炎Congenitalandperinatedinfection先天和圍產期感染MaybesevereFetalandnewbornCytomegalicinclusiondisease(巨細胞包涵體病)DeathoffetusC.N.Sdefect:耳聾，智力發(fā)育遲緩AnimportantpublicproblemViralantigensLatentphaseAgEBNA1:latentinfectionEBNA2:transformationofcellsLMP:latentinfectiontransformcellsReplicationAgEarlyAg:viralreplicationLateAg:structureproteins

PathogenecitySpearedbycontact:LatentinfectionPrimaryinfection:moreunapparentCelltransformationDiseasesInfectiousmononucleosisTransmittedbyinfectedsalivaMoreseeninadolescentsandyoungadultsFever,sorethroat,WBCincraesedDistinguishwithleukemiaheterophilagglutination(異嗜凝集試驗)：heterophilAbcanagglutinate