代謝組學研究技術及應用

1、 代謝組學研究技術及應用代謝組學研究技術及應用生物系生物系 趙闖營趙闖營 代謝組學主要研究技術代謝組學主要研究技術目目 錄錄什么是代謝組學什么是代謝組學 ? ? 代謝組學應用舉例代謝組學應用舉例 gene一一 mrna protein metabolite系系統(tǒng)統(tǒng)生生物物學學 一、什么是代謝組學一、什么是代謝組學 1.代謝組學的提出 jeremy k. nicholson教授作為代謝組學的先行者，被譽為國際“代謝組學之父”. metabonomics:the quantitative measurement of the multi-parametric metabolic response

2、of living systems to pathophysiological stimuli or genetic modifications. 生物系統(tǒng)對生理和病理刺激以及基因改變的代謝應答的定量測定. 2.代謝組學的概念 在現(xiàn)有的英文表述中，代謝組學同時存在兩個不同的詞匯和概念，即metabonomics和metabolomics. metabolomics存在多個定義，但其精髓是：對一個生物系統(tǒng)的細胞在給定時間和給定條件下所有小分子代謝物質(zhì)的定量分析 (the quantitative measurement of all low molecular weight metabolite

3、s in an organism cells at a specified time under specific environmental conditions). metabolomics 著重研究的是單個細 胞或細胞類型中所有的小分子成分和波動規(guī)律,或許可以叫做細胞代謝組學. 多用于植物和微生物系統(tǒng). metabonomics代謝組學是關于定量描述生物內(nèi)源性代謝物質(zhì)的整體及其對內(nèi)因和外因變化應答規(guī)律的科學. (metabonomics is the branch of science concerned with the quantitative understandings of t

4、he metabolite complement of integrated living systems and its dynamic responses to the changes of both endogenous factors (such as physiology and development) and exogenous factors (such as environmental factors and xenobiotics). 因此，metabolomics可以譯作代謝物組學.metabolomics是metabonomics的一個組成部分. 3. 3.代謝組學的發(fā)

5、展代謝組學的發(fā)展198219831984198919992000200120022007: publication of ms for urine profiling first publication on 1h-nmr of blood and plasmamulti-component analysis of spectra data from rat urinenicholson and wilson: nmr spectroscopy of biofluidsdefinition of metabonomics first independent pharma publication

6、 of metabonomics publication in nature on metabonomicsexplanation of statistics in metabonomics 二、代謝組學主要研究技術二、代謝組學主要研究技術 代謝組學分析流程代謝組學分析流程樣品的采集及預處理樣品的采集及預處理數(shù)據(jù)的采集數(shù)據(jù)的采集數(shù)據(jù)的分析及解釋數(shù)據(jù)的分析及解釋 代謝組學各分析流程技術代謝組學各分析流程技術 abstract proton-nmr-based metabonomics offers a rare opportunity as a definitive screening tech

7、nique for biofluids and tissue biopsies. the procedure is extraordinary in that it allows the complete biochemical picture to be examined at one time and is able to detect subtle but repeatedly consistent disparities that may be occurring in different, and perhaps unrelated, biochemical pathways. su

8、ch metabolic responses to an initial perturbation in homeostasis may be followed over a sequential time-course to their eventual dissipation or consequent sequelae. the application of this technique is beginning slowly to filter into the area of endocrine research and has been used to examine long-t

9、erm and diffuse physiological alterations that may occur following such events as anabolic steroid treatment of cattle and the exposure of endometrial cells to tamoxifen. although only modest inroads have been made so far, this technique promises immense potential for future researches within the en

10、docrine field. contents1. introduction 2. proton nuclear magnetic resonance spectroscopy 3. the metabonomic approach and pattern recognition 4. application within the biological field 5. hormones, drugs and the endocrine system references 1. what is metabonomics? metabonomics:the quantitative measur

11、ement of the multi-parametric metabolic response of living systems to pathophysiological stimuli or genetic modifications. (生物系統(tǒng)對生理和病理刺激以及基因改變的代謝應答的定量測定) 2. principle of proton-nmr 在特殊的磁場中，用無線電射頻脈沖激發(fā)氫原子核，引起氫原子核共振，并吸收能量。在停止射頻脈沖后，氫原子核按特定頻率發(fā)出射電信號，并將吸收的能量釋放出來，被體外的接受器收錄，經(jīng)電子計算機處理獲得圖像 3. 1h-nmr and methods of analysis nmr data nlm hca and pca . unsupervised simca n