NoncodingRNA非編碼RNA課件

1、NoncodingRNA非編碼RNA 1Repertor:Xiaoning Yuan Oct.9.2012Irnne.MP,Cheng G,Wieland.S,et al，2007NoncodingRNA非編碼RNANoncoding RNAvIntroductionvClassification vResearch method vMechanism vThe relationship with major diseaseNoncodingRNA非編碼RNA1.Introduction Noncoding RNA(ncRNA) is a kind of transcripts without

2、 the ability of coding proteins,which exist widely in highly eukaryotic.The amount of the ncRNAtranscripts is up to 98 of the whole genomes transcripts. The difference in molecular structure and expressive pattern from other kinds of RNA endows them specific biological functions. Although exact func

3、tions of most ncRNA are not well known.it has been demonstrated that ncRNA was associated with some human diseasesNoncodingRNA非編碼RNANoncodingRNA非編碼RNA2.Classificationv1830nt: miRNA，siRNA，piRNAv30200nt: rRNA，tRNA，snRNA， snoRNA，SLRNA，SRPRNA vMore than 200nt:mRNA-like noncoding RNA and so onNoncodingRN

4、A非編碼RNANoncodingRNA非編碼RNA3.Researching methodvMethod for identification: bioinformation establish specific cDNA libraries DNA Tiling chip NoncodingRNA非編碼RNAIdentificationNoncodingRNA非編碼RNA3.Researching methodvMethod for reserching function: Clustering methed Nock out NoncodingRNA非編碼RNA4. Mechanismva

5、. Noncoding RNA can interact with DNA, for example, noncoding RNA can modify chromosome configuration, and then affect transcription activity of local region of DNA.NoncodingRNA非編碼RNA4.Mechanismvb. Noncoding RNA can interact with mRNA to adjust the edition, stability and the translation of mRNA.Nonc

6、odingRNA非編碼RNA4.Mechanismvc. Noncoding RNA ,as a regulater for synergismcan,can bind to protein factor and change the biological activity of the protein. chemotatic noncoding RNA also recruit protein to specific site of a cell.NoncodingRNA非編碼RNA4.Mechanismvd.Noncoding RNA which overlap itself and be

7、 a complex higher-order structure,privide space or binding site for molecular and protein factor.vbesides,somebody hold the opinion that RNA molecular simimulate prion protein conversion.NoncodingRNA非編碼RNA5.The relationship with major diseasea.Neurological disease: schizophrenosis autism melancholia hyperactivity NoncodingRNA非編碼RNAThe relationship with major diseasevb.cancer The H19 noncoding RNA is essential for human tumor growth The signaling pathway mediated by ncRNA associate with hunman tumor growthNoncodingR