常見(jiàn)載體引物表

1、常見(jiàn)載體引物表VectorPrimer(F)Primer(R)PBADPBAD-F/TRXFPBAD-RpACTT7T3pAc5.1/V5-HisAc5-ProforBGHpACT2GAL4-AD-CforpGAL4-ADrvpAS2-1GAL4 BDpAS2-1.RpB42ADpB42ADFpB42ADRpBACPAK8BAC1BAC2pBK-CMVT7T3PBS(SK/KS)/M13-M13F/T7T3/M13RpBV220PBV220FPBV220RpCAMBIA 1301(1300)P1P2 (同 2.0-rev )pCAMBIA 2300M13R(-48)M13F(-47)PCANT

2、B5ES1/M13RS6pCAT3-e nhan cerRVP3此載體無(wú)反向引物pcDNA3.0CMV-F/T7SP6/BGHpcDNA3.1CMV-F/T7BGHpcDNA4T7/CMV-FBGHpcDNA6T7/CMV-F(J21025 在 T7 前面)BGHpcDNAIIT7SP6pCE2.1M13FM13RpCEP4pCEP-FEBV-RpCF-TM13FM13RpCIT7 (17Base)此載體無(wú)反向引物pCI-neoT7 (17Base)T3pCMS-EGFPT7T3pCMV-3Tag-4AT3 /PFLAG-CMV-FT7pCMV5pCMV5FpCMV5RpCMV5-FlagpC

3、MV5FpCMV5RpCMV-MYC/NUCCMV-FBGHPCMV-HACMV-FEBV-RpCMV-SportM13F/T7SP6/M13RpCMV-Tag(KAN+)T3T7pCR2.1-TOPOM13F/T7M13RpCR3.1T71BGHpCS2SP6T7pDonarM13FM13RpDONR221M13FM13RpDriveT7SP6pDRIveR(KAN+)T7SP6pDsRED1-C1pDsRED-ex-C1-FpEGFP-N-3'（距離很近，一般不 用）pDsRed1-N1CMV-ProforRFP-NrevpDsRed-Mo nomer-N1CMV-ProforDS

4、RED1 n primerpDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3pDSRED-N1(KAN+)pEGFP-N-5PDSRED-N-RpECFP-CpEGFP-C-5pECFP-C-3'pECFP-N1pEGFP-N-5pEGFP-N-3pEF/myc/ERpEF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5pEGFP-C-3'pEGFP-N(KAN+)pEGFP-N-5pEGFP-N-3pENTR/D-TOPO(KAN+)M13FM13RpET-*(His)(KAN+)T7T7 TerpET22(a,b,c)(AMP+

5、)T7T7 TerpET28,30,24,49(KAN+)T7T7 TerpET32(a,b,c)(AMP+)T7/S.tagT7 TerpET-42a (-b, -c)(+)StagT7 TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7 TerpEYFP-N1pEGFP-N-5pEGFP-N-3PETBluePETBlueT7UP太 靠前，在T7 前)/ PETBlueUPPETBlueDOWNpEYFP-C1pEGFP-C-5pEGFP-C-3'pFLAG-CMVCMV30CMV-24pGAD424GAL4 AD3'ADpGA

6、DGHGAL4 AD3'ADpGADT7-RecGAL4 AD/T73'ADpGAPZa-Aa-FACTOR3'AOXpGBKT7(Ka na+)T73'BDpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)TpGEX-4T-5'pGEX-4T-3'pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)Pi npoi ntTM VectorPinpoint PrimerSP6pIRESPIRES-F/T7T3/PIRES-R (J40720)plRES2-EGFPpIRES2-EGFP.P5pIRES2-EGFP.P3

7、pLXSNpLXSN-FpLXSN-RpMAL-c2EMalE primerM13F(-47)pMAL-C2xP5P3'pMAL-p2XMalE primerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19-TM13F(-47)M13R(-48)pPIC9K(AMP+ ZEO+5 ' AOX/a-factor3 ' AOXpPICZa5'AOX/PPICZa-F3'AOXpPROEXHTAM13R(-48)無(wú)反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSile ncer3.1-H1 hygr

8、oM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7”T3pSP72T7SP6pSport1SP6/M13F(-47)T7/M13RpSUPERT7T3pTA2M13F/T7(優(yōu)先使用 M13FT3/M13RpT-AdvM13F /T7M13RpTARGET TMpTarget.F(在 T7 前面)/T7M13R?pT0-T7T7T7TERpTRC99a-cpTRC99C-FpTRC99C-RpTriPLEx25'pTriPLEx2T7pTWIN-1T7T7TER客戶確認(rèn)再用）pUC18(19)/11

9、8(119)M13F(-47)M13R(-48)pUC57M13R(-48)/M13RM13F(-47)/M13FpUCm-TM13F/T7M13RpVAX1T7BGHpWSK29T7/M13F/M13F(-47)T3/M13R/M13R(-48)pXT7T7SP6pYES2T7pYes2.RpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpRECEIVERCMV-F此載體無(wú)反向引物pSHUTTLE-CMVPSHUTTLE-CMV-F”P(pán)SHUTTLE-CMV-RpSP64/65SP6此載體無(wú)反向引物pSTBIue-1T7SP6pT7Blue(R)T7M13F(-4

10、7)pRK5SP6pSG5-3' or SV40pA-RpMSCVMSCV5'MSCV-revpBABEpBABE5'pBABE3 'pFastBac HT A (B, C)pFastBac-FpFastBac-RpFastBac1PH-Profor/ BaculoVforpFastBac-RpACYCDuet-1 (MCS I)ACYCDuetUP 1DuetDOWN1pDSRED2-N1(KAN+)pEGFP-N-5DsRed1-NpFastBac Dual (MCSI)BaculoVfor/PHpFastBac-R/SV40-pArevpBSN-TT7T3

11、pHISi 1PHISi-1無(wú)pRSV-Rev（根據(jù)酶切位點(diǎn)選 擇不同引物）pREPF/M13FHIV-Rev-NES/ pGEX-3'EZ-TM13F(-47)M13R(-48)pMSCV-hygMSCV5'PMSCV-RMsCV-revPTHIOHIS (A )TRXF( new)M13FPDEST22PDEST22-FPDEST22-RpLXSNpLXSNf wpLXSNrvpLEXApLEXA-FpLEXA-RpCDHpCDH-EF1無(wú)PVXLBALBBpAcGP67 (A, B, C)PHpAcGP67rvpAD-TrackpAD-Track-F無(wú)PLPCPLPC-F/CMV-FPLPC-RpHAGE-CMV-MCS-IZsGreenpHAGE-CMV-F暫沒(méi)設(shè)計(jì)PLENTI-HISCMV-F無(wú)pDSRED-PRESS-C1pDSRED -C1-FpDSRED-C1-RpCR-BluntM13RM13F/T7pMT/V5-His A (B,C)MT-ProforBGHrevpEASY-T3T7/M13FSP6/M13RPSILENCE-1.0-U6T72.0REV( 同 PQpCAMBIA 1301)pLVX-IRES-ZsGreenlPIRES2-EGFP-FPIRES2-EGFP-RpDC316pDC316FpDC316RPMK0.1PLXS