D-Luciferin-D-minus-Luciferin-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemED-LuciferinCat. No.: HY-12591ACAS No.: 2591-17-5Synonyms: D-(-)-Luciferin; Firefly luciferin分式: CHNOS分量: 280.32作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/m

2、L (356.74 mM)H2O : 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.92 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.5 mg/mL (8.92 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 D-luciferin螢光素酶的天然底物，可催化物發(fā)光昆產(chǎn)典型的黃

3、綠光。體外研究 D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typicalyellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives oranalogues that are substrates or inhibitors of the luciferase from the Amer

4、ican firefly Photinus pyralis, theenzyme more frequently used in techniques of in vitro and optical imaging 1.體內(nèi)研究 Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as asubstrate is currently the most widely employed technique. The total signal inte

5、nsity is plotted against thetime after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals atfixed time points (5, 10, 15, and 20min) after D-luciferin injection are determined as alternatives to the peaksignal. The signal in a given time-intensity c

6、urve is normalized for the peak signal in the curve to representthe pattern of temporal changes after D-luciferin injection 2.PROTOCOLAnimal Mice 2Administration 2 In vivo BLI is performed using a cooled charge-coupled device camera system (IVIS Imaging System 100) 3,5, 7, 10, 12, 14, 19, 21, 24, an

7、d 28 days after the inoculation of HCT116-Luc cells. Mice are injected with 75mg/kg D-luciferin in 100L of phosphate-buffered saline subcutaneously near the scapula and were placedin the light-tight chamber of the imaging system under isoflurane anesthesia. Beginning 5min after injection,dorsal lumi

8、nescent images with an exposure time of 1s are acquired sequentially at a rate of one image permin until 20min after D-luciferin injection. Data acquisition is continued until 40min postinjection on days 3 or5 and until 25min on day 7, because of the prolonged time course of light emission. Binning

9、is 4 and the fieldof view is 15cm.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) J Leukoc Biol. 2019 Jun 18. Biomed Pharmacother. 2019 Sep;117:109126.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Giuseppe Meroni, et

10、al. D-Luciferin, derivatives and analogues: synthesis and in vitro/in vivo luciferase-catalyzed bioluminescent activity.ARKIVOC 2009 (i) 265-288.2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE2. Inoue Y, et al. Timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growthusing in vivobioluminescence imaging. Int J Biomed Imaging. 2010;2010:471408.McePdfHeightCaution: Product has not been fully