生物信息學(xué)分析課件methylation

1、主要內(nèi)容從GEO質(zhì)控歸一化差異甲基化位點(diǎn)差異甲基化區(qū)域注釋數(shù)據(jù)甲基化Illumina Human Methylation 450K BeadChip可組近450,000個(gè)甲基化位點(diǎn)，具有單堿檢測(cè)人全基的分辨率。全面覆蓋了96%的CpG島，并根據(jù)需求加入了CpG島以外的CpG位點(diǎn)、人類干細(xì)胞非CpG甲基化位點(diǎn)、正常組織與腫瘤（多種）組織差異甲基化位點(diǎn)、編碼區(qū)以外的CpG島、miRNA啟動(dòng)子區(qū)域和GWAS疾病相關(guān)區(qū)域的位點(diǎn)，同時(shí)覆蓋了Human點(diǎn)。Methylation27 BeadChip的90%的位的值A(chǔ)探針（非甲基化）的數(shù)目UB探針（甲基化）的數(shù)目M值或者m值m=log（ =M（M U 10

2、0）-1值反映了能夠和給定被甲基化的序列匹配的寡核苷酸的比率，序列中的甲基化率M值可以消除探針不同而造成的影響2015/6/15數(shù)據(jù)1. GEO2. TCGA、EBI等其它質(zhì)控質(zhì)控歸一化差異甲基化位點(diǎn)Iderceptfpvalqvalcg19229544-5.8198172499137.683047.18E-080.013840388cg137067693.1920619497427.7085051.09E-070.013840388ch.1.1262625R-16.609626056836.2767051.28E-070.013840388cg019723352.1445063985554.

3、8292211.94E-070.01571845cg19506433-0.652971394201.7085823.39E-070.02196957cg05945291-16.609626052652.5513598.51E-070.033324073cg21081097-6.2839646562621.4287148.71E-070.033324073cg080941352.3493027952547.0554339.22E-070.033324073cg25010146-5.2185579212449.9157499.97E-070.033324073熱圖差異甲基化區(qū)域chrstarten

4、dvalueareaclusterindexSt artindexE ndLcluster Lp.valuefwerchr125257191252582360.4341700338.68340066148661173211751203300chr11239720123979770.4238167838.05251888431573274030274048193700chr63193837231939112- 0.4346384127.38885300916398116387500chr6149805995149806732- 0.5585011816.143512994170492181364

5、0chr2209224225209225183- 0.6179528483.70771708611590073375733806800差異甲基化區(qū)域value-average smooth methylation difference within blockarea-block area estimate (abs(value) * length)cluster-id of cluster blockgroup within which block occursindexStart-index ofcluster in blockindexEnd-index of last cluster in blockL-number of clusters in blockclusterL-number of probes in blockp.value-permuion p.value based on difference conditioned on lengthfwer-family-wise error rate estimate based on difference conditioned on lengthp.valueArea-permu