CFSE-5-6-Carboxyfluorescein-diacetate-succinimidyl-ester-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECFSECat. No.: HY-D0938CAS No.: 150347-59-4Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester分式: CHNO分量: 557.46作靶點: Others作通路: Others儲存式: -20C, protect from light, stored under

2、nitrogen* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight, stored under nitrogen)溶解性數(shù)據(jù)體外實驗 DMSO : 62.5 mg/mL (112.12 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.7939 mL 8.9693 mL 17.9385 mL5 mM 0.3588 mL 1.7939 mL 3.5877 mL10 mM 0.1794 mL 0.8969 mL 1.7939 m

3、L請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實驗 請根據(jù)您的實驗動物和給藥式選擇適當?shù)娜芙獍?，配制前請先配制澄清的儲備液，再依次添加助溶?為保證實驗結(jié)果的可靠性，體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當天使；澄清的儲備液可以根據(jù)儲存條件，適當保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (3.73 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO

4、90% corn oilSolubility: 2.08 mg/mL (3.73 mM); Clear solution1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 CFSE是可以跟蹤細胞分裂的熒光染料。體外研究 CFSE is a fluorescent dye which can track the cell division 1. To examine the ability of immune cells tomigrate into live slices, a single-cell

5、suspension of autologous splenocytes or PBMC is isolated, labelingthem with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days ofculture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. Nopositive cells are found in th

6、e slices without the addition of CFSE-labeling immune cells 2.PROTOCOLCell Assay 2 Single-cell suspensions of splenocytes (or PBMC) are stained with 1 M CFSE in PBS for 9 min at 37C,combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted.2106 CFSE-labeling s

7、plenocytes are added to the top of the slices in 24-well membrane culture insert 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Cinthia C. Stempin, et al. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cr

8、uzi Infection. PLoSNegl Trop Dis. 2017 Jan; 11(1): e0005307.2. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.Oncoimmunology. 2017; 6(7): e1333210.McePdfHeightCaution: Product has not been fully validated for medical applications.Fo