Dihydrofluorescein-diacetate - Dye Reagent - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDihydrofluorescein diacetateCat. No.: HY-101893CAS No.: 35340-49-9分式: CHO分量: 418.4作靶點(diǎn): Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 106.5 mg/mL (254.54 mM)* means solubl

2、e, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.3901 mL 11.9503 mL 23.9006 mL5 mM 0.4780 mL 2.3901 mL 4.7801 mL10 mM 0.2390 mL 1.1950 mL 2.3901 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Dihydrofluorescein diacetate于細(xì)胞系統(tǒng)和細(xì)胞模型中測量氧化應(yīng)激的熒光探針。體外研究D

3、ihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a betterfluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizingspecies. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures,

4、 consistent withmitochondria, in reoxygenated endothelium 1. Dihydrofluorescein diacetate is able to detect the presence of1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filamentswhich corresponded in all

5、details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROSreleased in the matrix 2. Dihydrofluorescein diacetate could be an useful and quantitative method formeasuring the oxidative potential of nanoparticle-treated cells 3.PROTOCOLCell Assay Dihydrofluorescein diacetate is us

6、ed at a final concentration of 5 M for the cell-free studies. The excitationwavelength is 488 nm and the emission wavelength is 521 nm, the same as the excitation and emissionwavelengths of the confocal microscope. All fluorescence measurements are performed on roomtemperature solutions. Human umbil

7、ical vein endothelium at first passage are cultured to confluence on 48well plates. Cells are ished with PBS, then PBS with glucose, pH 7.4, is added to the cells.Dihydrofluorescein diacetate is added at a final concentration of 20 mM. Wells without cells are also loadedwith fluorescent probes to se

8、rve as controls. Fluorescence measurements are made on a fluorescentplatereader every 10 min at Ex 488 nm and Em 510 (bandpass 5 nm) and 538 nm (bandpass 10 nm). TheEm 510 nm and Em 538 nm values are added together 1.MCE has not independently confirmed the accuracy of these methods. They are for ref

9、erence only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Food Hydrocolloid. 2019 Feb.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2,7-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2,7-di

10、chlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free RadicBiol Med. 1999 Jul;27(1-2):146-59.2. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003Oct;120(4):319-25.3. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment ofnanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.McePdfHeightCaution: Product has not been fu