凝膠固定技術(shù)的生物組織傳感器研究

1、a-蘋果汁-溶膠-凝膠修飾電極在EP中b-Nafion-蘋果汁-溶膠-凝膠修飾電極在EP中c-Nafion-蘋果汁-溶膠-凝膠修飾電極在DA和EP混標(biāo)中5、分析應(yīng)用配制一系列濃度的多巴胺標(biāo)準(zhǔn)溶液，采納示差脈沖伏安法及利用Nafion-蘋果汁溶膠凝膠修飾電極對(duì)多巴胺標(biāo)準(zhǔn)溶液進(jìn)行測定，繪制工作曲線（圖6）。結(jié)果表明，多巴胺濃度在210-6 mol/L110-4 mol/L范圍內(nèi)響應(yīng)電流呈良好的線性關(guān)系。吸取多巴胺針劑溶液30L，用PBS定容配成25mL（即多巴胺濃度為6.3310-5 mol/L）。用本傳感器進(jìn)行定量分析，測得多巴胺的濃度為6.5810-5 mol/L，實(shí)驗(yàn)誤差為3.9%。參考文獻(xiàn)

2、1 蔡沛祥，黃昊，余新志，聶兵，“對(duì)多巴胺敏感的植物組織碳糊生物電極的改進(jìn)”， 傳感技術(shù)學(xué)報(bào)，1996,3, (1)：75-782 唐尹平，成鳳桂，“Sol-Gel固定法制作生物傳感器應(yīng)用及進(jìn)展”，中南民族學(xué)院學(xué)報(bào)，2000，3，19(1):89-933 何星存，李平，莫金垣，“用修飾電極導(dǎo)數(shù)伏安法同時(shí)測定多巴胺和腎上腺素”，分析測試學(xué)報(bào)，2000，1，19(1):19-214 汪振輝，郟建波，李工安，周漱萍，“多巴胺在聚茶堿Nafion雙層修飾玻碳電極上的電化學(xué)行為”，分析化學(xué)，2000,5,28(5):568-5725 鄧家祺，方躍，“植物組織電極”，化學(xué)傳感器，1990，10(3)：5-

3、106 沈國勵(lì)，陳光焰，水冰，“土豆組織膜兒茶酚電極的研究”，分析化學(xué)，1990，18(4)：315-319A Tissue Biosensor Based On Sol-gel Immobilization TechniqueXuan Jun, Chen Lixing, Xu Changliu(Class 2000, School of Chemistry and Chemical Engineering, Sun Advisor: Cai Peixiang, ProfessorAbstract：In this paper, a new technique on preparation of

4、 biosensor is reported. SiO2 sol-gel and plant juice are mixed a certain proportion to make the enzyme from nature plant immobilized in the SiO2 sol-gel film. The dopamine biosensor is prepared from a platinum electrode coated with a Nafion film and then the midified SiO2 sol-gel layer.Cyclic voltam

5、metry(CV) and Linear scan voltammetry are used to investigate the electrochemical characteristics of the sensor. The catalysis effect of different plant species and parts is compared. The dosage of ethanol, water, TEOS and plant juice is studied. The linearity of DA in the range of 210-6110-4mol/L w

6、ith detection limit of 8.010-7mol/L and the correlation coefficient of 0.999 are obtained by differential pulse voltammetry(DPV). No interference was found from ascorbic acid, nitrite, uric acid, epinephrine and noradrenalin in the determination of DA. Satisfactory determination results of DA in samples are obtain by t