TMPD-dihydrochloride-DataSheet-生命科學試劑-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemETMPDdihydrochlorideCat.No.:HY-W012145CASNo.:637-01-4分?式:C??H??Cl?N?分?量:237.17作?靶點:Others作?通路:Others儲存?式:PleasestoretheproductundertherecommendedconditionsintheCOA.溶解性數(shù)據(jù)體外實驗DMSO:16.67mg/mL(70.29mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM4.2164mL21.0819mL42.1638mL5mM0.8433mL4.2164mL8.4328mL10mM0.4216mL2.1082mL4.2164mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存?式和期限。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案，配制前請先配制澄的儲備液，再依次添加助溶劑(為保證實驗結果的可靠性，體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；澄的儲備液可以根據(jù)儲存條件，適當保存；以下溶劑前的百分?指該溶劑在您配制終溶液中的體積占?)：1.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥1.67mg/mL(7.04mM);ClearsolutionBIOLOGICALACTIVITY?物活性TMPDdihydrochloride，?種易于氧化的化合物，?種酶促轉化的氧化還原活性底物分?。TMPDdihydrochloride也?種電?供體，并作為?紅素過氧化物酶的還原共底物。TMPDdihydrochloride也?1/2MasterofSmallMolecules—您?邊的抑制劑?師www.MedChemE種complexIV的底物。體外研究TMPD(N,N,N′,N′-tetramethyl-para-phenylene-diamine)isusedincellcultureandmicrobiologytodifferentiateorganismsthatexhibitcytochromecoxidaseactivityandtodistinguishbetweenGram-negativeandGram-positivepathogenicandnon-pathogenicbacteria.TheconceptofTMPDoxidationbymicroorganismsiswidelyusedandisknownasasuccessfulcolorimetricindicatorforbacterialoxidases,astheradicalcationTMPD+˙,formedbyoxidation,showsacharacteristicdeepbluecolour.TheelectrochemicalrecognitionoftheTMPDoxidationbybacterialoxidasescanbeappliedtoavarietyofpathogens[1].REFERENCES[1].SKuss,etal.ElectrochemicalrecognitionandquantificationofcytochromecexpressioninBacillussubtilisandaerobe/anaerobeEscherichiacoliusingN,N,N',N'-tetramethyl-para-phenylene-diamine(TMPD).ChemSci.2017Nov1;8(11):7682-7688.[2].NenadPetrovic,etal.UsingN,N,N',N'-tetramethyl-p-phenylenediamine(TMPD)toassaycyclooxygenaseactivityinvitro.MethodsMolBiol.2010;594:129-40.[3].FrancoARossato,etal.Fattyacidsynthaseinhibitorsinduceapoptosisinnon-tumorigenicmelan-acellsassociatedwithinhibitionofmitochondrialrespiration.PLoSOne.2014Jun25;9(6):e101060.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchu