Acetylcysteine-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEAcetylcysteineCat.No.:HY-B0215CASNo.:616-91-1分?式:C?H?NO?S分?量:163.19作?靶點:ReactiveOxygenSpecies;EndogenousMetabolite;Apoptosis;Ferroptosis;InfluenzaVirus作?通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB;Apoptosis;Anti-infection儲存?式:4°C,protectfromlight,storedundernitrogen*Insolvent:-80°C,6months;-20°C,1month(protectfromlight,storedundernitrogen)溶解性數(shù)據(jù)體外實驗H2O:50mg/mL(306.39mM;Needultrasonic)掃描?維碼，運?溶解?案計算器獲得適合您實驗體系的溶解?案MassSolvent1mg5mg10mgConcentration制備儲備液1mM6.1278mL30.6391mL61.2783mL5mM1.2256mL6.1278mL12.2557mL10mM0.6128mL3.0639mL6.1278mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存?式和期限。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶1.請依序添加每種溶劑：PBSSolubility:120mg/mL(735.34mM);Clearsolution;Needultrasonic1/3www.MedChemEwww.MedChemEBIOLOGICALACTIVITY?物活性Acetylcysteine(N-Acetylcysteine)?種粘液溶解劑(mucolyticagent)，可?于減少粘液的度。Acetylcysteine?種ROS抑制劑。Acetylcysteine半胱氨酸前體，通過中和花?四烯酸依賴的5-脂氧合酶活性所產(chǎn)?的毒性脂質(zhì)來防??紅素誘導(dǎo)的鐵中毒(ferroptosis)。Acetylcysteine可以誘導(dǎo)細(xì)胞凋亡(apoptosis)，并具有抗流感病毒活性。IC50&TargetHumanEndogenousMetabolite體外研究AcetylcysteinepreventsapoptoticDNAfragmentationandmaintainslong-termsurvivalintheabsenceofothertrophicsupportinserum-deprivedPC12cells.AcetylcysteinealsopreventsdeathofPC12cellsandsympatheticneurons[2].Acetylcysteinecausesdose-dependentreductionsinviabilityinratandhumanaorticsmoothmusclecells[3].AcetylcysteineactivatestheRas-extracellularsignal-regulatedkinase(ERK)pathwayinPC12cells.Acetylcysteineprotectsneuronalcellsfromdeathevokedbywithdrawaloftrophicsupport.Acetylcysteineincreasesnitricoxide(NO)releasefromprotein-boundstoresinvasculartissue.AcetylcysteinepretreatmentofPC12cellsinterfereswithNGF-dependentsignalingandneuriteoutgrowth,anditissuggestedthatAcetylcysteineinterfereswithredox-sensitivestepsintheNGFmechanism[4].體內(nèi)研究Acetylcysteine(150,300mg/kg)treatmentsignificantlyreduceslivertransaminasesinallgroupsoftreatment,mostlyingroupAcetylcysteine300.LungglutathioneperoxidaseissignificantlyincreasesingroupAcetylcysteine300(P=0.04),whiletheotheroxidationbiomarkersshownosignificantdifferences[6].Acetylcysteineimprovescognitionof12-month-oldSAMP8miceinboththeT-mazefootshockavoidanceparadigmandtheleverpressappetitivetaskwithoutinducingnon-specificeffectsonmotoractivity,motivationtoavoidshock,orbodyweight[5].PROTOCOLCellAssay[2]Forsurvivalexperiments,washedcellsareresuspendedinRPM11640mediumandplatedin0.5mLatadensityof8-10×105perwellin24wellplasticculturedishescoatedwithrattailcollagen.Tofeed,buttoavoidlossoffloatingcells,freshmedium(0.2mL)isaddedtotheculturesondays1,5,and10.Forexperimentsinvolving"primed"PC12cells,culturesarepretreatedforl-2weekswithNGFinRPM11640mediumsupplementedwith1%heat-iN-acetylcysteinetivatedhorseserum.Thecellsarethenwashedandpassagedintoserum-freeRPM11640medium.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalRats:RatsarerandomLyallocatedintofivegroups:shamgroup(n=5),controlgroupwithIIR(n=8)andthreeAdministration[6]groupswithIIRwhoaregivenAcetylcysteineindifferentdosages:150mg/kgintraperitoneally5minbeforeischemia(n=8,groupAcetylcysteine150),300mg/kgi.p5minbeforeischemia(n=7,groupAcetylcysteine300),and150mg/kgi.p5minbeforeischemiaplus150mg/kg5minbeforereperfusion(n=7,groupAcetylcysteine150+150).After4hofreperfusion,theanimalsareeuthanizedbyexsanguinationfromtheabdominalaorta.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.2/3www.MedChemEwww.MedChemE戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellMetab.2019Dec3;30(6):1107-1119.e8.?SignalTransductTargetTher.2020May8;5(1):51.?SciAdv.2021Apr14;7(16):eabb2213.?Biomaterials.2020Mar;233:119753.?RedoxBiol.2019Jun;24:101215.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].HalasiM,etal.ROSinhibitorN-acetyl-L-cysteineantagonizestheactivityofproteasomeinhibitors.BiochemJ.2013Sep1;454(2):201-8.[2].FerrariG,etal.N-acetylcysteine(D-andL-stereoisomers)preventsapoptoticdeathofneuronalcells.JNeurosci.1995Apr;15(4):2857-66.[3].TsaiJC,etal.InductionofapoptosisbypyrrolidinedithiocarbamateandN-acetylcysteineinvascularsmoothmusclecells.JBiolChem.1996Feb16;271(7):3667-70.[4].YanCY,etal.PreventionofPC12celldeathbyN-acetylcysteinerequiresactivationoftheRaspathway.JNeurosci.1998Jun1;18(11):4042-9.[5].FarrSA,etal.Theantioxidantsalpha-lipoicacidandN-acetylcysteinereversememoryimpairmentandbrainoxidativestressinagedSAMP8mice.JNeurochem.2003Mar;84(5):1173-83.[6].KalimerisK,etal.N-acetylcysteineamelioratesliverinjuryinaratmodelofintestinalischemiareperfusion.JSurgRes.2016Dec;206(2):263-272.[7].GariglianyMM,etal.N-acetylcysteinelacksuniversalinhibitoryactivityagainstinfluenzaAviruses.JNegatResultsBiomed.2011May9;10:5