H2DCFDA-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEH2DCFDACat.No.:HY-D0940CASNo.:4091-99-0分?式:C??H??Cl?O?分?量:487.29作?靶點(diǎn):ReactiveOxygenSpecies作?通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB儲(chǔ)存?式:-20°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥150mg/mL(307.82mM)掃描?維碼，Ethanol:14.29mg/mL(29.33mM;Needultrasonic)運(yùn)?溶解?案計(jì)算器H2O:<0.1mg/mL(insoluble)獲得適合您實(shí)驗(yàn)體系的溶解?案*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.0522mL10.2608mL20.5217mL5mM0.4104mL2.0522mL4.1043mL10mM0.2052mL1.0261mL2.0522mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存?式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶1.請依序添加每種溶劑：10%DMSO40%PEG3005%Tween-8045%salineSolubility:2.08mg/mL(4.27mM);Suspendedsolution;Needultrasonic此?案可獲得2.08mg/mL(4.27mM)的均勻懸濁液，懸濁液可?于?服和腹腔注射。以1mL?作液為例，取100μL20.8mg/mL的澄DMSO儲(chǔ)備液加到400μLPEG300中，混合均勻；向上述體系中加?50μLTween-80，混合均勻；然后繼續(xù)加?450μL?理鹽?定容?1mL。1/3www.MedChemEwww.MedChemE2.請依序添加每種溶劑：10%DMSO90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(4.27mM);Clearsolution此?案可獲得≥2.08mg/mL(4.27mM，飽和度未知)的澄溶液。以1mL?作液為例，取100μL20.8mg/mL的澄DMSO儲(chǔ)備液加到900μL20%的SBE-β-CD?理鹽??溶液中，混合均勻。BIOLOGICALACTIVITY?物活性H2DCFDA(DCFH-DA)?種可滲透細(xì)胞的，?于檢測細(xì)胞內(nèi)活性氧(ROS)的探針[1]。體外研究Guidelines(Followingisourrecommendedprotocol.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds).1.H2DCFDAisdissolvedinDMSOtoobtaina10mMstocksolutionsandfurtherdilutedbeforeuse.2.Cellsareincubatedwith5μMH2DCFDAsolutioninPBSinthedarkfor30minat37°C,thenharvestedwith0.05%trypsin-EDTAsolution,suspendedinafreshmedium,andimmediatelyanalyzedwithflowcytometer.3.AlongwiththeH2DCFDAprobe,ifindicated,useROS-insensitivemodificationofthefluoresceindyeDCFDAasapositivecontrol.ThestainingprocedureisthesameasfortheH2DCFDA[1].PROTOCOLKinaseAssayROSMeasurements[1]ForthedetectionofintracellularROSlevel,ROS-sensitiveprobeH2DCFDAisused.Adherentcells(ESCs,difESCs,eMSCs,HeLa,U118)areincubatedwith5μMstainingsolutioninPBSinthedarkfor30minat37°C,thenharvestedwith0.05%trypsin-EDTAsolution,suspendedinafreshmedium,andimmediatelyanalyzedwithflowcytometer.Lymphocytes,bothcontrolandPHA-activated,areresuspendedinPBS,incubatedwith5μMofH2DCFDAinthedarkfor30minat37°C,andimmediatelyanalyzed.AlongwiththeH2DCFDAprobe,ifindicated,ROS-insensitivemodificationofthefluorescentdyeDCFDAisused.ThestainingprocedureisthesameasfortheH2DCFDA[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?JHazardMater.2021Mar15;406:124306.?ACSApplMaterInterfaces.2020Jul8;12(27):30145-30154.?ACSApplMaterInterfaces.2020Mar18;12(11):13360-13370.?ACSApplMaterInterfaces.2019Sep25;11(38):34663-34675.?CellRep.2019Jul2;28(1):78-90.e6.Seemorecustomervalidationsonwww.MedChemEREFERENCES2/3www.MedChemEwww.MedChemE[1].LyublinskayaOG,etal.Redoxenvironmentinstemanddifferentiatedcells:Aquantitativeapproach.RedoxBiol.2017Aug;12:758-769.McePdfHeight關(guān)注MCE中國公眾號，

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