半滑舌他課件

Molecularmarker-assistedsexcontrolinaquaticproduct半滑舌鰨

To：Half-smoothtonguesoleAPCRmethod（afemale-specificAFLPmarker(CseF305)）foridentifyinggeneticsexofthesolewasdeveloped.Effectsofmethyltestosteronetreatmentonthefemale---phenotypicmales.Threecombinationsofsexchromosomes(ZZ,ZWandWW)wereobservedintheneo-maleprogeny

DNAextractionfromtissuePhenotypicsexidentificationbyhistologicalsectioningPhenotypicsexidentificationbyhistologicalsectioningCloningofthefemale-specificAFLPmarkerCseF305andsequenceanalysisGeneticsexidentificationusingfemale-specificSCARmarkersInductionofsexreversaloffrybyandrogenMaterialsandmethodsCulturingandreproductionmanipulationofneo-malesChromosomeanalysisofneo-males'progenyfryDataanalysis

DevelopmentofgeneticsexidentificationusingthemarkerCseF305CseF305N1:CTCCCCTGACCTTCCTTTCseF305C1:5'-CGGCAGCACAATTATTACA-3'Inductionofsexreversalbyandrogens

Geneticsexidentificationofsex-reversedtonguesoleThegeneticsexoffryobtainedfromthecrossingofneo-malesandnormalfemaleswasidentified.Theproportionofgeneticallyfemaleindividualsinprogenyfromthecrossingofneomalesandnormalfemaleswas73.4%,whichwas23%morethanthatofnormalfry本文思路的由來

半滑舌鰨雌魚比雄魚生長快，體積大

激素表觀性不宜分別，基因鑒定分子標(biāo)記CseF305已經(jīng)找到半滑舌鰨雌魚特有的基因分子標(biāo)記育種的應(yīng)用對魚進(jìn)行PCR和跑膠證實(shí)雄魚中沒有此基因，雌魚有此基因，且基因片段長度為160bp用不同濃度MT進(jìn)行處理觀察其性別比

對新型雄魚進(jìn)行熒光標(biāo)記，然后從被標(biāo)記的雄魚中取樣進(jìn)行性別基因鑒定64條魚中有47條為雌魚73.4%，比正常繁育產(chǎn)生的雌魚高出23%。

從F2代魚苗中選4-6cm長的魚苗進(jìn)行染色體核型檢測，發(fā)現(xiàn)三種組合型（ZZ,ZW,和WW）超級(jí)雌性個(gè)體含有兩個(gè)大的W異性染色體。WW雌性的染色體核型。雌雄基因序列已經(jīng)全部測出來了

Genbank序列分析他們（雌雄）中的基因序列不同之