Congo-Red-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemECongoRedCat.No.:HY-D0236CASNo.:573-58-0分?式:C??H??N?Na?O?S?分?量:696.66作?靶點:Others作?通路:Others儲存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數(shù)據(jù)體外實驗DMSO:20mg/mL(28.71mM;Needultrasonic)H2O:<0.1mg/mL(insoluble)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.4354mL7.1771mL14.3542mL5mM0.2871mL1.4354mL2.8708mL10mM0.1435mL0.7177mL1.4354mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2mg/mL(2.87mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2mg/mL(2.87mM);ClearsolutionBIOLOGICALACTIVITY?物活性CongoRed?種偶氮染料。CongoRed結(jié)合已被?泛?于組織切?中淀粉樣蛋?的測定。體外研究CongoRedhistochemicalstainmayserveasasimplescreeningtoolforinvestigatingiftheaggregatesinmutantcellshavemisfoldedβ-pleatedsheetsecondarystructures.CongoRedhistochemicaldyehastheabilitytobindspecificallytocrossedβ-pleatedsheetstructures.Wild-typeHSPB1shouldmaintainproteinhomeostasisbybindingproteinsinnon-nativeconformations,therebypreventingsubstrateaggregation.TheT139Mmutant,however,failsinthisfunctionandresultsinanaccumulationofmisfoldedproteins,whicharetargetedbyCongoRedforintercalatingbetweentheβ-pleatedsheetstructures.CongoRedhistochemicalstainmayserveasasimpletooltoinvestigateiftheaggregatesinmutantcellshavemisfoldedβ-pleatedsheetsecondarystructures[1].PROTOCOLCellAssay[1]HeLacellsareselectedforthesestudiesduetotheirlargecytoplasmicvolume.Cellstransfectedwithmutantorwild-typeHSPB1constructsaregrownoncoverslipsfor24hrandthenarestainedwithCongoredtodetermineiftheaggregatesdisplayamyloidogenicproperties.Briefly,cellsarefirstfixedwith10%formalinfor10minandstainedwith1%Congoredfor5min,followedbydestainingwith0.01%potassiumhydroxidein50%ethanol.Coverslipsarethenpassedthroughgradedethanolconcentrationsfordehydrationandmountedinamountingmediumandexaminedbyfluorescentmicroscopyunderrhodaminefilter[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻?EurJPharmacol.2022Dec2;175446.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].AmornvitJ,etal.Anovelp.T139MmutationinHSPB1highlightingthephenotypicspectruminafamily.BrainBehav.2017Jul21;7(8):e00774.McePdfHeightCaution:Producthasnotbeenfullyvalidated