BCA蛋白濃度測(cè)定試劑盒(增強(qiáng)型)

BCA蛋白濃測(cè)定試劑盒增強(qiáng)型BCA白濃度測(cè)定試劑盒(增強(qiáng)型)BCA白濃度測(cè)定試劑盒(增強(qiáng)型)產(chǎn)品編號(hào)產(chǎn)品名稱(chēng)包裝蛋白濃度測(cè)定試劑盒(增強(qiáng)型次產(chǎn)品簡(jiǎn)介：BCA白濃度測(cè)定試劑盒(增強(qiáng)型)(EnhancedBCAProteinKit)是根據(jù)目前世界上最常用的兩種蛋白濃度檢測(cè)方法之一BCA法制而成，實(shí)現(xiàn)了蛋白濃度測(cè)定的簡(jiǎn)單，高穩(wěn)定性，高靈敏度和高兼容性。和碧云天生產(chǎn)的普通白濃度測(cè)定試劑盒相比，靈敏度更高，檢測(cè)濃度下限達(dá)到10g/ml，最小檢測(cè)蛋白量達(dá)到，待測(cè)樣品體積為1-20l和碧云天生產(chǎn)的普通白濃度測(cè)定試劑盒相比，顯色速度更快，相同的樣品孵育較短時(shí)間即可進(jìn)行吸光度測(cè)定。在20-1000g/ml濃度范圍內(nèi)有較好的線性關(guān)系。測(cè)定蛋白濃度不受絕大部分樣品中的化學(xué)物質(zhì)的影響，可以兼容樣品中高達(dá)的，的X-100，的80但受螯合劑和略高濃度的還原劑的影響，需確保低10mM無(wú)，二硫蘇糖醇低于，β-巰基乙醇低于0.01%。不適用BCA法建議試用碧云天生產(chǎn)的Bradford白濃度測(cè)定試劑盒(P0006)BCA白濃度測(cè)定試劑盒(增強(qiáng)型)對(duì)樣品中各種物質(zhì)的詳細(xì)的兼容性和普通的白濃度測(cè)定試劑盒相同，請(qǐng)參考碧云天如下網(wǎng)頁(yè):

ForKit.pdf每個(gè)試劑盒可以檢測(cè)5000樣品。包裝清單：產(chǎn)品編號(hào)產(chǎn)品名稱(chēng)包裝試劑瓶，共P0009-2劑P0009-3蛋白標(biāo)準(zhǔn)管，共2管P0009-4蛋白標(biāo)準(zhǔn)配制液―說(shuō)明書(shū)保存條件：室溫保存。蛋白標(biāo)準(zhǔn)配制成溶液后凍存。注意事項(xiàng)：需酶標(biāo)儀一臺(tái)，測(cè)定波長(zhǎng)為540-595nm之間，最佳。需96孔板。如果沒(méi)有酶標(biāo)儀，也可以使用普通的分光光度計(jì)測(cè)定，但測(cè)定時(shí)，需根據(jù)比色皿的最小檢測(cè)體積，適當(dāng)加大作液的用量使不小于最小檢測(cè)體積，樣品和標(biāo)準(zhǔn)品的用量可相應(yīng)按比例放大也可不變。使用分光光度計(jì)測(cè)定蛋白濃度時(shí)，每個(gè)試劑盒可以測(cè)定的樣品數(shù)量可能會(huì)顯著減少。如發(fā)現(xiàn)樣品稀釋液或裂解液本身就有較高背景，請(qǐng)?jiān)囉帽淘铺焐a(chǎn)的Bradford蛋白濃度測(cè)定試劑盒(P0006)。為了加快測(cè)定蛋白濃度的速度可以適當(dāng)用微波爐加熱，但是切勿過(guò)熱。度必須小于，不兼容。不適用BCA法時(shí)，請(qǐng)?jiān)?/p>

用碧云天生產(chǎn)的Bradford蛋白濃度測(cè)定試劑盒(。為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。使用說(shuō)明：1.取1.2ml蛋白標(biāo)準(zhǔn)配制液加入到一管蛋白標(biāo)準(zhǔn)(30mg中，充分溶解后配制成蛋白標(biāo)準(zhǔn)溶液。配制后可立即使用，也可以長(zhǎng)期保存。2.取適量25mg/ml蛋白標(biāo)準(zhǔn)，稀釋至終濃度為。例如取25mg/ml蛋白標(biāo)準(zhǔn)，加入980l稀釋液即可配制成白標(biāo)準(zhǔn)。蛋白樣品在什么溶液中，標(biāo)準(zhǔn)品也宜用什么溶液稀釋。但是為了簡(jiǎn)便起見(jiàn)，也可以用0.9%NaCl或稀釋標(biāo)準(zhǔn)品。稀釋后的蛋白標(biāo)準(zhǔn)也可以長(zhǎng)期保存。3.根據(jù)樣品數(shù)量，按50積BCA劑1體積試劑B(50:1)配制適量作液，充分混勻。例如5mlBCA劑A100lBCA試B混勻，配制成BCA工作液。作液室溫24時(shí)內(nèi)穩(wěn)定。4.將標(biāo)準(zhǔn)品按0,4,20l加到96板的標(biāo)準(zhǔn)品孔中，加標(biāo)準(zhǔn)品稀釋液補(bǔ)足到20l5.加適當(dāng)體積樣品到96板的樣品孔中，加標(biāo)準(zhǔn)品稀釋液到20l6.各孔加入作液，37℃置鐘。注:也可以室溫放置2時(shí)，或℃置30鐘。測(cè)定蛋白濃度時(shí)，顏色會(huì)隨著時(shí)間的延長(zhǎng)不斷加深。并且顯色反BCA白濃度測(cè)定試劑盒(增強(qiáng)型)

應(yīng)會(huì)因溫度升高而加快。如果濃度較低，適合在較高溫度孵育，或適當(dāng)延長(zhǎng)孵育時(shí)間。7.測(cè)定，間的波長(zhǎng)也可接受。根據(jù)標(biāo)準(zhǔn)曲線計(jì)算出樣品的蛋白濃度。常見(jiàn)問(wèn)題：1.測(cè)定標(biāo)準(zhǔn)曲線時(shí)發(fā)現(xiàn)隨著標(biāo)準(zhǔn)品濃度的增加吸光度或顏色沒(méi)有明顯變化?？赡艿脑蚴菢悠分泻袊?yán)重干擾測(cè)定蛋白濃度的物質(zhì)，詳細(xì)的的兼容性列表請(qǐng)參考碧云天如下網(wǎng)頁(yè):ForBCAKit.pdf2.是否每次測(cè)定時(shí)都需要做標(biāo)準(zhǔn)曲線？建議每次測(cè)定時(shí)都做標(biāo)準(zhǔn)曲線。因?yàn)闇y(cè)定時(shí)顏色會(huì)隨著時(shí)間的延長(zhǎng)不斷加深，并且顯色反應(yīng)的速度和溫度有關(guān)，所以除非精確控制顯色反應(yīng)的時(shí)間和溫度，否則如需精確測(cè)定宜每次都做標(biāo)準(zhǔn)曲線。使用本產(chǎn)品的文獻(xiàn)：1.WangLiuWuasanearlyintheofinducedwoodfordinIinK562ToxicolPharmacol.2022Jan2.ChenDF,LiuMJ,Dualeffectsofinduceditscombinationwith

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