2015年藥學(xué)大會(huì)暨第十五屆藥師e3黃

黃1,2,（1.山東省中,濟(jì)南250014;2.山東中大學(xué),濟(jì)南國內(nèi)外相關(guān)文獻(xiàn)對急性肝損傷模型的進(jìn)行整理、分析與歸納。結(jié)果目前常見的模型多治療急性肝損傷藥物的合理評價(jià)。為了提供更好的保肝中藥的評價(jià)工具，應(yīng)在中醫(yī)理ApplicationofAnimalModelsofAcuteLiverInjuryHUANGNa-na1,2,SUN（1.Shacademyofmedicine,Ji’nan250014;2.ShUniversityofTraditionalMedicine,Ji’nan250355）s：ObjectiveTosummarizetheresearchprogressonALIcausedbydifferentfactorsofanimalmodelsinrecentyears.MethodsReferencesinthelastdecadesbothathomeandabroadaboutALImodelwerecollated,yzedandsummarized.ResultsAtpresentthecommonmodelismodernpathologymodel(modelofALIismainlychemical,drug,andalcoholandotherautoimmune),butfewstudieshavereportedananimalmodelofTCMSyndrome,andallofthesearenotfullyreflectthenatureofthesediseasesinALI,thusalsoaffectsthereasonableevaluationofdrugsforacuteliverinjury.ConclusionInordertostudythetraditionalmedicinewithprotectliverfunctionprovidesabetterevaluationtoolforevaluation,weshouldbeundertheguidanceof medicinetheorytomanifestationsofALIonthebasisofclinicalmedicine,combiningmodernetiology,machinestudydisease,researchmoreinlinewithactualclinicalsyndromesandALIanimalmodelofTCMtheory.，:Acuteliverinjury;Animalmodel;Building， 通訊作者：，女，研究員·博導(dǎo)，研究方向：中藥藥理與毒理， .化學(xué)性肝損傷模[4]。以腹腔內(nèi)注射500~850mg/kg體重的D-GalN的方法造成急性肝損傷所需劑量因動(dòng)物機(jī)體功能狀態(tài)機(jī)實(shí)驗(yàn)?zāi)康牟煌植町怺1]。等[5]給wistar大鼠腹腔注射藥物性肝損傷模給小鼠腹腔注射溶于0無菌生理鹽水的A00~500gg。等9]實(shí)驗(yàn)對雜種家犬采用醋氨酚多點(diǎn)皮射，首次劑量為5gkg體重，并在9h和2h分別進(jìn)行第2次和第3次注射，用量均為200gkg體重，模型成功率為76.72330)。低劑量的A不會(huì)造A--NAQGH的解毒能力，于是未被清除的NAI與生物大分子共價(jià)結(jié)合，導(dǎo)致蛋白質(zhì)巰基被氧化祿保[10]實(shí)驗(yàn)研究認(rèn)為180gkg劑量異煙肼灌胃8h后能成功誘導(dǎo)小鼠急性肝損傷模型[1]為用400gk異煙肼續(xù)灌胃1可制成大肝損傷型有究認(rèn)為異煙肼的代謝(乙酰偶氮或其分解產(chǎn)物)與肝細(xì)內(nèi)的大分子(蛋白質(zhì)、核酸)共價(jià)結(jié)合，給小鼠尾靜脈注入0.2ml卡介苗溶液，12天后每天經(jīng)尾靜脈注入7.5μgLPS生理鹽水溶急性性肝損傷動(dòng)物模等[21]曾對一組大鼠用56°的紅星二鍋頭每日給大鼠灌胃0.7ml白酒/100g體重·次，2ml/100g18%的乙醇溶液（分析純無水乙醇加無菌生理鹽水），10天造成大鼠急性肝損害，且灌胃效果比腹腔內(nèi)注射要好很多。等[22]用不同濃度（1%、3%、5%、8%）的白酒給大鼠灌胃，并用含18.2%玉激怒20min，以后每隔1天，增加10min，連續(xù)20天。實(shí)驗(yàn)第21天，給大鼠腹腔注ALI模型因素結(jié)合誘導(dǎo)形成ALI模型，然后反過來從中醫(yī)證候的角度去探尋急性肝損傷的發(fā)病機(jī)理，[1]XUSY，BIANRL.Experimentalmethodologyofpharmacology(藥理實(shí)驗(yàn)方法學(xué))[MVol3.People'sMedicalPublishingLIUFP，SHILL.CCl4causingacuteliverinjuryinaratmodel[J].ChinJBloodTransfusion(中國輸血雜BergerML，BhattH，CombesB，etal.CCl4?inducedtoxicityinisolatedhepatocytes：Theimportanceofdirectsolventinjury[J].Hepatology，1986，6(1)：36-45.LUX，WANGTL，ZHAOJB，etal.RatModelofliverinjuryinducedbyD-galactosamine[J].JournalXUZN，WUXM，WANGP，etal.18αglycyrrhizicacidinthetreatmentofacuteliverinjuryinducedby—galactosamineinrats[J].JournalofClinicalHepatology(臨床肝膽病)，2012，28(1)：63-HUANGZM，YANGXB，CAOWB，etal.MethodsStudyontheModelofChemicalHepatic([J].PharmJ 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