白骨壤NAC家族成員鑒定和功能初探

[32]。RT-qRCR鑒定AmNAC基因在鹽脅迫處理條件下的表達情況發(fā)現(xiàn)，鹽脅迫下，白骨壤葉中有5個基因均在處理24h時呈現(xiàn)上調表達，并且基因AmNAC4被顯著誘導，表達量約為對照組的3倍。這表明它們可能參與白骨壤應答鹽脅迫過程，其中AmNAC4可能起關鍵作用。RT-qRCR結果與轉錄組數(shù)據不完全一致，可能是由于樣品生長環(huán)境不同所致。已有多項研究證實，NAC轉錄因子通過調控鹽脅迫相關基因的表達水平，引發(fā)一系列復雜的脅迫反應，從而提高植物應對高鹽環(huán)境的能力。在植物鹽脅迫調控中，這些NAC轉錄因子主要通過依賴ABA途徑、不依賴ABA途徑或兩者共同參與的方式發(fā)揮作用REF_Ref163408844\r\h[33]。例如依賴ABA途徑的水稻OsNAC3基因，通過調控ABA響應和耐鹽相關基因的表達，提高植株對ABA的敏感性，從而增強植株的耐鹽性REF_Ref163408898\r\h[34]。AmNAC基因共表達網絡結果顯示，5個鹽脅迫響應AmNACs分別與多個白骨壤基因呈現(xiàn)共表達趨勢。以上結果表明，作為轉錄因子，AmNACs可能通過調控下游基因的表達進而調控白骨壤鹽脅迫響應過程。但AmNAC轉錄因子參與鹽脅迫響應的途徑及下游靶標基因的篩選仍有待進一步研究。本研究的成果為篩選受鹽脅迫誘導的靶標基因、開展后續(xù)的遺傳轉化研究以及深入探索鹽脅迫響應功能和分子調控機制奠定了理論基礎。

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