Dynamic Light Scattering Measuring latelet Quality and Bacterial 動態(tài)光散射測量血小板質(zhì)量和細(xì)菌

1、2 of 17 platelet transfusions used to treat and/or prevent bleeding number of platelet transfusions produced per year: canada: 300,000united states: 2,000,000europe: 2,500,000 platelets are sensitive to stress (e.g., cold damage) activated platelets do not help patients.storage at 22c to prevent col

2、d damageincreased risk for bacterial growthshortages - addressed by increasing donor recruitment3 of 17the problemclinical situation solving the problem novel approachdata acquisitiondls-pm score the inventiontechnology backgroundthe dls-pmaccomplishmentsclinical results summary other technology acc

3、uracy, sensitivity4 of 17no non-invasive in vitro test available tested inventory cannot be transfused platelet quality / bacterial testing cannot be mandatedgood outcome assumption: good plateletsbad outcome assumption: bad plateletstransfusion outcome serves as retrospective platelet quality indic

4、ator. 5 of 17 at least 20% discard rate additional 10% discarded after testing (invasive technology) unknown quality of transfused platelets $1,000 / transfusion worldwide loss of at least 1 billion dollars per yearin summary: unknown platelet quality is a safety issue discarded platelet transfusion

5、s are a financial burden6 of 17multi-dimensional quality measurementdls-pm detects discoid platelets activated platelets microparticles bacteria at the same time7 of 17platelets50-100 nm1200 nm bacteria (if present)500 nmmicroparticlestransfus med rev 2007; 21(4):2958 of 17most fresh platelet transf

6、usionsmost platelet transfusions until day 7 - 12 of storagetemperatureresponsemicroparticlesplateletsize9 of 17 non-invasive small sample volume fast inexpensivephys med biol 2006; 51:374710 of 17may - aug 2007first clinical pilot study2006validationlatex beadsplateletsbacteriaproteinsnanoparticles

7、sept 2005first generation instrumentapril 2005first prototypenov 2003 proof of principle11 of 17dls-pm score vs. transfusion merit12 of 17104/160 transfusions65 %transfusion meritcorrelates with dls-pm score 13 of 17 invasive no single instrument can determine platelet quality and function in multip

8、le dimensions impedance optical immunofluorescence hematology analyzers cannot assess bacterial contamination bact/alert requiredsysmex xe-2100 kobeadvia 120siemens facscanto iibdbact/alertbiomrieux14 of 170204060801001201401601802001234without beadswith beadsbeads15 of 17optical methods - low sensi

9、tivity especially for platelet counts 15,000 / lsegal et al. brj haematol 2005; 128:520.16 of 17v mandated technology for hospitals / blood banksv 10,000 hospitals / blood banks in north americav extended shelf life - reduced outdate-discard ratev patient safetyv monitor manufacturing processesdls-pm: the only technology for noninvasive bacterial detection and platelet quality testing at the same timethank you!keddie brown, icaptureaudrey labri