川芎嗪對兔腎缺血再灌注損傷超微結(jié)構(gòu)改變的干預作用 醫(yī)學論文

1、川芎嗪對兔腎缺血再灌注損傷超微結(jié)構(gòu)改變的干預作用 醫(yī)學論文 本文由中國論文范文收集整理。 【摘要】 目的:觀察兔腎缺血再灌注損傷超微結(jié)構(gòu)改變，探討川芎嗪注射液的干預作用。 方法 :復制家兔腎缺血再灌注損傷模型，日本大耳白兔30只，隨機均分為三組：假手術(shù)組（S），缺血再灌注組（IR）和缺血再灌注+川芎嗪注射液組（LZ）。使用透射 電子 顯微鏡觀察各組腎組織標本超微結(jié)構(gòu)的變化，作對比 分析 。結(jié)果:IR組主要異常改變有：腎小球毛細血管腔狹窄，中性粒細胞附壁，大部分內(nèi)皮細胞核固縮、染色體邊集，細胞質(zhì)內(nèi)空泡形成，線粒體空泡變性。多數(shù)臟層上皮細胞輕度核固縮，個別線粒體空泡變性，足突相互黏連，可見局灶性融

2、合現(xiàn)象和微絨毛化現(xiàn)象。近曲小管上皮細胞核固縮，胞質(zhì)內(nèi)出現(xiàn)較多空泡，溶酶體和致密顆粒沉積增多，線粒體數(shù)量減少，部分線粒體嵴斷裂、空泡化，腔面微絨毛稀疏并有輕度腫脹現(xiàn)象。LZ組大部分標本的上述部位存在不同程度損傷性改變，但比IR組均明顯減輕，其中近曲小管上皮細胞超微結(jié)構(gòu)的改善較顯著。結(jié)論:川芎嗪注射液可減輕腎缺血再灌注損傷所致的腎小球和腎小管超微結(jié)構(gòu)的異常變化。 【關鍵詞】 腎臟 缺血再灌注損傷 超微結(jié)構(gòu) 川芎嗪注射液 Abstract: Objective: To observe the ultrastructural changes in renal tissue during renal i

3、schemia reperfusion injury in rabbits and study the interfering effects of ligustrazine injection. Methods: The model of renal ischemia reperfusion injury of rabbit was established in vivo. Thirty rabbits were divided into three groups randomly: sham operation group (S group), ischemia reperfusion i

4、njury group (IR group) and ischemia reperfusion injury plus ligustrazine injection group (LZ group).The ultrastructural changes in renal tissue were observed under electron microscope in 5 hours after reperfusion. Results: The abnormal ultrastructure in IR group included that The cavities of the glo

5、merular capillary narrowed, polymorphonuclear leucocytes adhered to the wall of the veessel, most of the endothelia showed nucleus pyknosis and staining thick, chromosome gathered near to nuclear membrance, vacuolization in cytoplasm and some mitochondia vacuolar degeneration were seen. Light degree

6、 nucleus pyknosis demonstrated in most of the visceral layer of the epithelia, individual mitochondrial vacuolar degeneration, foot process mutual adhesion were seen with focal coalescence and microvillus. epithelial cells of proximal convoluted tubule appeared nucleus pyknosis, more vacuolization w

7、as seen in cytoplasm, lysosome and dense granule in cytoplasm increased, number of mitochondrial vacuolization decreased, its crest partly fragmented, microvilli rarefaction and swelling were found. The ultrastructural injuries of the renal tissues in LZ group were obviously alleviated as compared t

8、o IR group and improvement of ultrastructure in epithelial cells of proximal tubule was notable. Conclusion: Ligustrazine injection can mitigate the abnormal changes of ultrastructure in glomerulus and tubules during renal ischemia reperfusion injury. Key words: kidney; ischemia reperfusion injury;

9、ultrastructure; ligustrazine injection 腎臟是高血流量器官，其血流量約占心輸出量的1/41/3，容易發(fā)生腎缺血再灌注損傷（renal ischemia reperfusion injury, RIRI）。RIRI是臨床上腎移植、低血容量性休克等發(fā)生急性腎功能衰竭的主要病理生理基礎，因此，深入揭示其損傷性變化并尋求防治方法十分重要。本 研究 復制實驗兔RIRI模型，觀察腎組織超微結(jié)構(gòu)改變，并探討常用中藥川芎嗪注射液的作用。 1 材料和方法 1.1 模型制備 健康日本大耳白兔30只，雌雄不拘，體重1.72.7 kg，平均2.18 kg，由溫州醫(yī)學院實驗動物中心

10、提供。經(jīng)耳緣靜脈注射25%烏拉坦(4 ml/kg)麻醉，仰臥位固定于恒溫手術(shù)臺上，頸靜脈插管滴注生理鹽水0.51.5 ml/min。上腹部備皮、行正中切口，約5 cm，沿腹白線逐層分離，剪開腹膜，用濕紗布撥開腸管，暴露腎臟，玻璃分針分離腎動、靜脈，用無創(chuàng)動脈夾夾閉左、右腎動脈，阻斷血流1 h，然后松開動脈夾恢復血供5 h，復制腎缺血再灌注損傷模型。 1.2 實驗分組 動物隨機分為三組：缺血再灌注組(ischemia reperfusion，IR，n=10)，上述模型在夾閉腎動脈和恢復腎動脈血流時，自耳緣靜脈注射生理鹽水4 ml。缺血再灌注+川芎嗪注射液組(ischemia reperfusion+ ligustrazinn，LZ，n=10)，夾閉腎動脈和恢復血流即刻，從耳緣靜脈注射川芎嗪注射液（200 mgl/kg溶于4 ml生理鹽水，山東長富潔凈藥業(yè)有限公司生產(chǎn)，批號03041711），其余同IR組。假手術(shù)組(sham operation，S，n=10)，開腹后，僅暴露腎臟6 h，不阻斷腎動脈，余同IR組。 關鍵詞:改變,干預,作用,結(jié)構(gòu),