拉曼光譜應(yīng)用介紹.

1、拉曼光譜的應(yīng)用ThermoThermo FisherThermoThermo Fisher拉曼應(yīng)用characteristic Raman frequenciescomposition of material 物質(zhì)的組成同分異性體， 同分異構(gòu)體 相同元索不同拉曼頻率的確認(rèn)結(jié)構(gòu)mchanges in frequency of Raman peak 拉曼峰位的變 化 width of Raman peak 拉曼峰寬stress/strain State張力f應(yīng)力高分子纖維拉伸 薄膜退火溫度 半導(dǎo)體器件mquality of crystal 晶體質(zhì)量晶體化程度aintensity of Raman

2、peak 拉曼峰強(qiáng)度amount of material 物質(zhì)總量薄膜厚度！拉曼光譜除了鑒別物質(zhì)種類，主要測量分子振動頻率，定 量了解分子間作用力和分子內(nèi)作用力情況，并推斷分子的 對稱性，幾何形狀，分子中原子排列。拉曼應(yīng)用無機(jī)材料：很多無機(jī)材料是中心對稱的振動，紅外光譜不敏感。而拉 曼光譜只有很明顯的優(yōu)勢。高分子材料：單體結(jié)構(gòu)測定，結(jié)品度，It架分獷解析，組分分析，裂解原位研究，纖維拉伸應(yīng)力應(yīng)變，催化材料：表而吸附研究（排除基體擾，原位條件卜.（島溫、高1K）催化過程動力學(xué)研究，分獷篩席架結(jié)構(gòu)分析，物相變 化納米材料：納米尺度的表而效應(yīng)，體積效應(yīng)，邑獷尺寸效應(yīng)以及物理 特性（如碳管的半導(dǎo)體特性

3、與金屬效應(yīng)）都會在拉處光譜上 得到體現(xiàn)生命科學(xué)：蛋白質(zhì)、核酸、磷脂在疋常生理條件下的結(jié)構(gòu)、構(gòu)相。藥物與細(xì)胞作用，Thermo< 11 n t I r I cThermo FisherSCIENTIFIC拉曼應(yīng)用司法鑒定，理化分析、汕漆.橡膠.纖維.冷品，爆炸物.文檢，痕跡檢驗 考古/藝術(shù)品：分析顏料、銹蝕氧化物、青銅器、玉器、陶瓷、粘七、般品 地質(zhì)/珠寶：珠宋鑒定，礦物分析，包裹體成分分析，礦物隨溫度壓力和變 藥學(xué)： 晶空檢驗、原材料輔料分析鑒別、藥片成分分布，候選新約篩選，假藥鑒別，雜質(zhì)分析，注射液成分分析，溶出度分析半導(dǎo)體：應(yīng)力/應(yīng)變，表而損傷，摻雜等環(huán)境科學(xué)：溫空氣體與燃燒氣體與建

4、筑物反應(yīng)研究，人氣污染，重金屬污染碳材料：納米碳管，石墨，碳纖維，DLC膜，C60y 卩其實幾乎所有的紅外用戶都有購買拉曼光譜儀的可能性！ThermoThermo Fisher應(yīng)用市場分析ThermoThermo FisherThermoThermo Fisherfblymers1%Tobacco1%Gems-1%AutomotrvcSefncocxiuctor 2%、刃Art and Archaeotogy Aberglass 2% "I 1%S»eel1%ThermoThermo FisherContract Research 1%Agricultural Chcmic

5、afe 1%Rubber 2%Glass 1%Agrfculture 2%-Nuclear R>wer 2%ThermoThermo FisherThermoThermo FisherFrsonal Care Roducts 2%Food and Beverage 2% 一Rrtrolcum 1%Aerospace1%Rinting 1%.Environmental 1%j Consumer opbcs-'ctectrooicsGovcfnmen|/&b3%Chemical8%Ftedcal Dagnosbcs2%Ptarmaceutcal16%Thermo* <

6、11 N T I r I cThermo FisherSCIENTIFIC司法科學(xué)文檢不同復(fù)印機(jī)墨的拉 曼光譜比對ThermoThermo Fisher司法科學(xué)文檢>»« BUX« W «««HP laserjet 3020不同激光打印機(jī)墨 的拉曼光譜比對 ne1 Konica714Laserjet 6LThermo» < 11 N T I r I cThermo» < 11 N T I r I cThermo» < 11 N T I r I cThermo FisherSCIEN

7、TIFICThermo» < 11 N T I r I c司法科學(xué)理化檢驗Thermo» < 11 N T I r I cThermo» < 11 N T I r I c汕漆漆片拉 曼光譜Thermo» < 11 N T I r I cThermo FisherSCIENTIFICThermo* c 11 n r i r i c司法科學(xué)文檢不同纖維拉曼光譜780nm激發(fā)ThermoC 11 M T I r I cThermo FisherSCIENTIFIC司法科學(xué)文檢SirMtelMilMRTIkAinfrtiliielhePe

8、r pohllRirMnMknt 780 nm laser 10 sec exposure time 2 exposuresThermo FisherSCIENTIFICThermo* c 12 n r i r i c司法科學(xué)理化分析Ihenno FisherThermoC 11 N V I v13Ihenno FisherThermoC 11 N V I v#TNT Specimen 785 nm laser 10 sec on ds collectionThermotk < 11 n t i r i cMThermo Fisher SCIENTIFICIhenno FisherThe

9、rmoC 11 N V I v#司法科學(xué)-理化Ihenno FisherThermoC 11 N V I v#Ihenno FisherThermoC 11 N V I v#言 SU3Sse£Ihenno FisherThermoC 11 N V I v14司法科學(xué)賣點自動準(zhǔn)直光路，自動曝光，自動校正，自動扌II除熒光。真正的自動化是該領(lǐng)域的最大賣點！強(qiáng)調(diào)可以分析小于的痕量樣品以及樣品區(qū)域，可分 析現(xiàn)場殘留的微最物。強(qiáng)人的數(shù)據(jù)庫，深入拓展用戶的應(yīng)用！暗場照明低波數(shù)50cm有利于漆片，爆炸物等無機(jī)顯微鏡H鏡，排掉JY的LabRam-HR強(qiáng)大的售后服務(wù)！合適的價格可以推廣到局一級的單位！

10、ThermoThermo FishertcitNTiric13SCIENTIFIC文物考古書畫考古：(1)印泥成分鑒定:a.b.真品,c贋品1,d廣品2Thermo FisherThermo14文物考古書畫（2）拉曼光譜分析:ab真品，c. 廣品1,d. 贋品2Thermo文物考古青銅器優(yōu)點 1.青銅銹蝕物的原位分析2.微量取樣分析3多層銹蝕物的顯微形貌觀察及結(jié)構(gòu)分析國內(nèi)外有很多拉曼儀器研究人員曾涉及青銅及梵銹蝕物方面的研究, 美國密西根州立人學(xué)的Lowell I.McCan n教授6曾對中國東漢的 一件青銅搖錢樹的腐蝕情況作了非常系統(tǒng)的研究，并報告了幾種常 見青銅銹蝕產(chǎn)物的拉曼光譜圖。國內(nèi)云

11、南楚雄師范學(xué)院的上怡林等Thermo FisherThermo17Thermo FisherThermo#文物考古青銅器見青銅銹蝕物一覽表顏色銹蝕礦物綠色堿式碳酸銅(孔雀石)CuCO3-Cu(OH)2堿式氯化銅(氯銅礦或副氯銅礦)CuCI2-3Cu(OH)2藍(lán)色堿式碳酸銅(藍(lán)銅礦)2CuCO3-Cu(OH)2堿式硫酸銅2CuSO4-3Cu(OH)2-H2O紅色氧化亞銅(赤銅礦)Cu2O黑色氣化銅CuO白色碳酸鉛PbCO3堿式碳酸鉛2PbCO3-Pb(OH)2錫石SnO2其它Cu2SCuSThermoThermo Fisher citnr ir ic17SCIENTIFIC文物考古-腐蝕兀代斷劍

12、I：的銹蝕物Thermo文物考古-顏料WE紅色赤01礦Fe2O3wnPb3O4朱砂 HgStT 1 (red earth)Fe2O3貼輪規(guī)L"；枚 »加色幺鋼礦CuCI2-3Cu(OH)2IWI*b|i«W4i卻 CuCO3Cu(OH)2»故色石育CuCO3Cu(OH)2漢般與漢紫毎麻離時航«91色am緞01礦KU力解石骨白鉛白黃色維MPbO紅色：朱砂在激尤卜腸于變九不艷定. 恿色 «飲礦莊就）t卜44產(chǎn)生變化. n&i wnsit拉蟻侑'；很刪.薊色，人色色混雜A.匕 “不總疋“董朮勺対效應(yīng).Thermo* c 11

13、 n v I r I cThermo Fisher文物考古-顏料Thermo22Thermo#文物考古技術(shù)推廣自動準(zhǔn)直光路，自動曝光，自動校匚 自動扌II除熒光。水平光路！光纖探頭！強(qiáng)人數(shù)據(jù)庫!顯微鏡暗場照明!低波數(shù)50cm-1 Yf利于氧化物分析至少80級的激光功率調(diào)節(jié),顯微鏡冃鏡，排掉J丫的LabRam-HR強(qiáng)人的售后服務(wù)!Thermo FisherThermo# Diam ond sp3bonded carbon Graphite sp2bonded carbon碳材料拉曼光譜 Sheet of aromatic rings Fullere nes Hollow carb on sphe

14、res Diamond-like Carbon (DLC) coatings Amorphous Carbon Properties similar to Diamond Sometimes hydroge nated Different forms sometimes called polymorphs More accurately allotropesThermoThermo Fisher碳材料Graphe ne Single layers from Graphite structure Graphene nan oribb oris (GNR) roll up to form nano

15、 tubesRoll-upSWNT SW-CNT MW-CNT Carbon nano wallsgraphene sheet Walled graphene structuresThermo FisherThermo24碳材料 Spectra are well characterized by RamanThermo< 11 n v I r I cThermo< 11 n v I r I cThermoThermo< 11 n v I r I c碳材料-RBM波段（呼吸模）表征單壁碳管管徑間接農(nóng)征于性與電特性Thermo< 11 n v I r I c對激發(fā)波長很敏感

16、Thermo FisherSCIENTIFICThermo< 11 n v I r I c碳材料 G Bands (Tangential or Graphite bands) Centered around 1580 cm*1 Represent C-C stretching mode Good for determining electrical property (metallic or semic on ducting) Broadened G-band indicates metallic碳材料 D bands (Diamond or Defect bands) Centered

17、 around 1335 cm" Indicate defects in SW-CNTs碳材料拉曼光譜 Rama n can be used to compare and con trast nano tubes for the purposes of fine-tuning a synthesis processThermo* c 11 n v i r i cThermo* c 11 n v i r i c two carbon peaks graphitic "G” disordered carb on MDn correlati ons D/G area/inte n

18、sity ratioThermoThermo Fisher otNf ir IC29SCIENTIFIC碳材料DLC模拉曼光譜correlates with wear resistance G band area and intensitycorrelates with thicknessG band position correlates with wear resistance correlates with hydrogenThermo* c 11 n v i r i c碳材料1M0 2XC IQ ®Aj1 .01003 2000 3000 4000 6000Afefvenim

19、ber (cm*)300nHG. 3. R>m«in scattering %pc:tra taken from difT;rcnt regions of an individual 8 pm lonj TGC M the sharp tip (a); 2.5 (b); 5 (c); and 7.5 “m (d) away fron the tip.Large-sized tubular graphite cones with nano tube tips.APPLIED PHYSICS LETTERS 87,163102 2005Thermo Fisher32Thermo F

20、isher#ThermoThermo F isher碳材料賣點自動準(zhǔn)直光路，口動曝光，自動校E, 口動扌II除熒光。低波數(shù)50cnvW利于呼吸模的測量至少80級的激光功率調(diào)節(jié)，保證了碳管不會在激光熱效 應(yīng)下發(fā)生應(yīng)力或者相變！633n m激發(fā)是首選！Thermo Fisher#Thermo Fisher#ThermoThermo Fisher#約學(xué)晶形鑒別晶形鑒別5-Methyl-2-(2-nitrophe nyl)ami no -3-thiophenecarbonjtrilealso known as uROYn *Thermo Fisher34Thermo Fisher#Yu. L.: St

21、強(qiáng)hen&on, G. A: Milcheli. C. A.: BunnL C. A.: Snow匕 S, V：Bowvg J, J.: Bcrchardl. T, B.: Slowell, J G: Bym. S R. J. Am. Own. Soc 2000, 122, 585ThermoThermo Fisher33SCIENTIFIC藥學(xué)晶形鑒別Thermo Fisher#Thermo Fisher#ThermoThermo Fisher#藥學(xué)晶形鑒別約學(xué)低波數(shù)信息 Spectrum of Amino Acid - Cystine Low frequency bands in

22、Raman Shift Raman analytical signal occurs near wavelength of the laser where easily sensed by the detector Far IR equipment not required藥學(xué)賣點自動準(zhǔn)直光路，口動曝光，口動校止，自動抑除熒光。性價比很高的Smart DXR Variable Dynamic Point Sampling 快速動態(tài)取樣掃描Thermo FisherSCIENTIFICThermo» c 11 n v i r i c39Thermo FisherSCIENTIFICTh

23、ermo» c 11 n v i r i c#微孔板轉(zhuǎn)盤式樣吊附件可分析液體注射液低波數(shù)50cm-1 Yf利于藥物的低波數(shù)測量解偏激光器|The Well-Plate AutosamplerThermo FisherSCIENTIFICThermo» c 11 n v i r i c#Solar PanelsThermos c 11 n r i r i cThermo Fisher38SCIENTIFIC太陽能材料 Crystalline Silicon High efficiency in IR only Expe nsive Ages quickly Amorphous

24、 Silicon Lower efficiency (no IR) Shows little aging Inexpensive Tandem cells of Micro-crystalline and amorphous Silicon High efficiency(iDeluding IR) Shows no agingEcono micalThermo FisherSCIENTIFICThermo» c 11 n v i r i c40太陽能材料 Morphology CharacterizationThermo FisherThermo41Crystalline Sili

25、con BandS4O5fiOSOO40O4eOMO42O«OThermoThermo FishericitMtieic40SCIENTIFICThermo FisherThermo#太陽能材料Thermo FisherThermo#Thermo FisherThermo#Po«ltton (rrtcrc<ne«te<5)Amorphous Silicon:BlueMicrocrystaline Silicon:RedThermo FisherThermo42工業(yè)客戶賣點自動準(zhǔn)直光路，自動曝光，自動校止，自動抑除熒光。件價比很高的Smart DXR,

26、DXR microscope強(qiáng)大的軟件功能，TQ定量分析等強(qiáng)大的售后服務(wù)！Thermo FisherThermo43Thermo* c 11 n v I r I c42Thermo FisherSCIENTIFICThermo FisherThermo#摻雜對拉曼光譜影響Thermo FisherThermo#Thermo FisherThermo#2004006008001000Raman shift (cm-1)3 RuiTKtn xpoulra of (he cenunics v%iih difkrvnt Sb conlcnK.422=lii(K044NaC.52Li004)(Nb080

27、xTa020Sbx)O3 piezoelectric ceramicsSbO的鍵能(372 kJ/mol)要比NbO的鍵能(753 kJ/mol)低J. Am. Ceram. Soc., 91 72211-2216 (2008)Thermo FisherThermo44結(jié)晶度Raman Shift (cm*1)Thermo FisherSCIENTIFICThermoC 11 N T I r I cThermo FisherSCIENTIFICThermoC 11 N T I r I cRaman spectra of crystalline and amorphous polyethylen

28、e terephthalate films.ThermoC 11 N T I r I cThermo FisherSCIENTIFIC聚合物生長Thermo FisherSCIENTIFICThermoC 11 N T I r I cThermo FisherSCIENTIFICThermoC 11 N T I r I cJ. Am. Chem. Soc. 2008, 130 (24), 7702-7709Thermo FisherSCIENTIFICThermoC 11 N T I r I c環(huán)境科學(xué)：溫室與燃燒物氣體對建筑材料的腐蝕CO2, SOx與 NOx氣體CaOHydrationpr

29、oc."STEP 1Atmosphetic AaCO attackC a(OH2 C aC O$2 5Te 1 (CakMe endfo* Ar»QO«wt. t>o9ymorptw»c forvn«)CaSO4.2H2O(Gypsum)MKl/OfCaSO4(AntwdntelCaNO,4HQ(Nitrcxuilcito)Thermo FisherThermo47FQiiro 6. OvotaII dwgradAtion proc:*a for calcium (And mAgn««itirn or t>ari

30、um) comrxMinciHbrtckAtmosphericCO. attackHydrationprocessNa2CO3.10H2O(Nttron)Na2SO4.2H2O* (Mirabiltte) *and/orNa:SO4(Then.dgNaNO,(NHratine>Thermo FisherThermo#Tl Figure 7. Overall degradation process for sodium compounds.環(huán)境科學(xué)溫室與燃燒物氣體對建筑材料的腐蝕研究Table 1 Raman characteristic bands &: strong, m: m

31、edium. w: weak v: very) of original materials and common degradation products arid pollutants affecting to stone rnatwials of Built HeritageCompoundFormulaWavcnumtxrs/cm 1GypournCaSOj 2HQ1132 tn, 1008 » 673 m( 619 n, 4M2 mz 413 inAnhidriteGaSO41160 w, 1130 mr 1112 w. 1017 vsf 675 m, 627 m, 60Q m“ 50) m, 417 mMirabiliteNa?SOj 10H2O1152 m, 1132 m, 1102vs, 645 m, 632 m,620 m, 466 w, 458 m.452 wThenarditeNa2SO41780 w, 1712 w, 1320 w. 1258 m, 1201 w188 w, 1155 w. 1136 w. 1105 w.995 vs, 626 m, 432 mCalciteGaLCh1436 wr 1085 vs, 712 m,2«l mAragoniteCaCCh1463 vw, 1085 vs, 705 rr, 205 m