文獻閱讀大黃素抑制乳腺癌肺轉(zhuǎn)移的機制研究

1、Emodin suppresses pulmonary metastasis of breast cancer accompanied with decreased macrophage recruitment and M2 polarization in the lungs（大黃素抑制乳腺癌肺轉(zhuǎn)移，伴隨著巨噬細胞募集和M2極化的減少） Xuemei Jia，F(xiàn)ang Yu，Junfeng Wang，et al. Breast Cancer Res Treat；(2014) 148:2913022022-3-251文獻閱讀文獻閱讀研究背景oTAM promote tumor growth an

2、d progression through extracellular matrix remodeling, promotion of tumor cell migration and invasion, angiogenesis, and immune suppression.oMAMs(metastasis-associatedmacrophages) provided survival and growth signals to metastatic tumor cells through binding of a4-integrin to VCAM-1 on tumor cells.o

3、Emodin suppresses the growth of various cancers through inducing cell cycle arrest, promoting apoptosis, or inhibiting angiogenesis. 2022-3-253文獻閱讀文獻閱讀研究背景oAs a pleiotropic molecule, Emodin affects the expression of several critical inflammatory factors including NF-kB, TNFa, iNOS, IL-10, and CXCR4.

4、 However, if and how Emodin exerts anti-tumor effects Through modulating inflammatory microenvironment or acting on immune cells has not been reported .2022-3-254文獻閱讀研究思路2022-3-25文獻閱讀5巨噬細胞與乳腺癌的轉(zhuǎn)移密切相關(guān)大黃素如何通過調(diào)節(jié)調(diào)節(jié)炎性微環(huán)境或者免疫細胞參與抗腫瘤作用，未見報道通過通過阻斷阻斷Sema3A/Nrp1Sema3A/Nrp1信號信號是否可以是否可以抑制巨噬細胞進入抑制巨噬細胞進入腫瘤腫瘤低氧區(qū)低氧

5、區(qū)，從而，從而起到起到抗抗血管生成并恢復(fù)抗血管生成并恢復(fù)抗腫瘤腫瘤免疫的作用？免疫的作用？大黃素抗腫瘤，同時參與調(diào)節(jié)炎性分子（TAM相關(guān)）Results：1. Emodin suppressed pulmonary metastasis of breast cancer cells2022-3-257文獻閱讀文獻閱讀Results：1. Emodin suppressed pulmonary metastasis of breast cancer cells2022-3-258文獻閱讀Results： 2. Emodin did not cause cytotoxicity to breast

6、 cancer cells or alter their migration at concentration achieved in vitro2022-3-259文獻閱讀 CELL CYTOTOXICITY(100uM) CELL MIGRATION(50uM、100uM)Results：3. Emodin reduced the accumulation of total and M2 macrophages in the lungs2022-3-2510文獻閱讀2022-3-25文獻閱讀1Results：3. Emodin reduced the accumulation of tot

7、al and M2 macrophages in the lungsResults：4. Emodin inhibited macrophage migration toward tumor conditioned medium2022-3-2511文獻閱讀Results：4. Emodin inhibited macrophage migration toward tumor conditioned medium2022-3-25文獻閱讀1文獻閱讀Results：5. Emodin suppressed macrophage M2 polarization2022-3-2512文獻閱讀Results：5. Emodin suppressed macrophage M2 polarization2022-3-25文獻閱讀1Results：6. Emodin inhibited STAT6 and C/EBPb signaling pathways in macrophages2022-3-2513文獻閱讀2022-3-25文獻閱讀1VIVOMetastatsis GrowthVITRO Cancer cell CYTOTOXICITY Cancer cel