文獻(xiàn)閱讀大黃素抑制乳腺癌肺轉(zhuǎn)移的機(jī)制研究

1、Emodin suppresses pulmonary metastasis of breast cancer accompanied with decreased macrophage recruitment and M2 polarization in the lungs（大黃素抑制乳腺癌肺轉(zhuǎn)移，伴隨著巨噬細(xì)胞募集和M2極化的減少） Xuemei Jia，F(xiàn)ang Yu，Junfeng Wang，et al. Breast Cancer Res Treat；(2014) 148:2913022022-3-251文獻(xiàn)閱讀文獻(xiàn)閱讀研究背景oTAM promote tumor growth an

2、d progression through extracellular matrix remodeling, promotion of tumor cell migration and invasion, angiogenesis, and immune suppression.oMAMs(metastasis-associatedmacrophages) provided survival and growth signals to metastatic tumor cells through binding of a4-integrin to VCAM-1 on tumor cells.o

3、Emodin suppresses the growth of various cancers through inducing cell cycle arrest, promoting apoptosis, or inhibiting angiogenesis. 2022-3-253文獻(xiàn)閱讀文獻(xiàn)閱讀研究背景oAs a pleiotropic molecule, Emodin affects the expression of several critical inflammatory factors including NF-kB, TNFa, iNOS, IL-10, and CXCR4.

4、 However, if and how Emodin exerts anti-tumor effects Through modulating inflammatory microenvironment or acting on immune cells has not been reported .2022-3-254文獻(xiàn)閱讀研究思路2022-3-25文獻(xiàn)閱讀5巨噬細(xì)胞與乳腺癌的轉(zhuǎn)移密切相關(guān)大黃素如何通過調(diào)節(jié)調(diào)節(jié)炎性微環(huán)境或者免疫細(xì)胞參與抗腫瘤作用，未見報(bào)道通過通過阻斷阻斷Sema3A/Nrp1Sema3A/Nrp1信號信號是否可以是否可以抑制巨噬細(xì)胞進(jìn)入抑制巨噬細(xì)胞進(jìn)入腫瘤腫瘤低氧區(qū)低氧

5、區(qū)，從而，從而起到起到抗抗血管生成并恢復(fù)抗血管生成并恢復(fù)抗腫瘤腫瘤免疫的作用？免疫的作用？大黃素抗腫瘤，同時(shí)參與調(diào)節(jié)炎性分子（TAM相關(guān)）Results：1. Emodin suppressed pulmonary metastasis of breast cancer cells2022-3-257文獻(xiàn)閱讀文獻(xiàn)閱讀Results：1. Emodin suppressed pulmonary metastasis of breast cancer cells2022-3-258文獻(xiàn)閱讀Results： 2. Emodin did not cause cytotoxicity to breast

6、 cancer cells or alter their migration at concentration achieved in vitro2022-3-259文獻(xiàn)閱讀 CELL CYTOTOXICITY(100uM) CELL MIGRATION(50uM、100uM)Results：3. Emodin reduced the accumulation of total and M2 macrophages in the lungs2022-3-2510文獻(xiàn)閱讀2022-3-25文獻(xiàn)閱讀1Results：3. Emodin reduced the accumulation of tot

7、al and M2 macrophages in the lungsResults：4. Emodin inhibited macrophage migration toward tumor conditioned medium2022-3-2511文獻(xiàn)閱讀Results：4. Emodin inhibited macrophage migration toward tumor conditioned medium2022-3-25文獻(xiàn)閱讀1文獻(xiàn)閱讀Results：5. Emodin suppressed macrophage M2 polarization2022-3-2512文獻(xiàn)閱讀Results：5. Emodin suppressed macrophage M2 polarization2022-3-25文獻(xiàn)閱讀1Results：6. Emodin inhibited STAT6 and C/EBPb signaling pathways in macrophages2022-3-2513文獻(xiàn)閱讀2022-3-25文獻(xiàn)閱讀1VIVOMetastatsis GrowthVITRO Cancer cell CYTOTOXICITY Cancer cel