組胚實(shí)驗(yàn)英文課件

1、39Section of rabbit ovary： H-E stain The ovary is elongated ovoid structure，the large vesicle bulging to the free surface is a mature follicle.Cross section of the ovary ,Low power Surface epithelium tunic albugineaCortex : ovarian follicles at various stage of development,the large mass called corp

2、us luteumMedulla: contain LCT and blood vessels is pushed to the periphery of the ovary.corpus albicans : composed of dense connective tissue white in color.High magnification:1a.superficial epithelium1b.tunica albuginea： consists of spindle shaped fibroblasts.2.cortex :2a. Primordial follicles：The

3、primary oocyte withA single layer of flattened follicular cells. 2b. Primary follicle large primary oocyte with single or some layers of follicular cells.The zona pellucida is red in colour and located between the follicular cells and the oocyte. 1a1b2a2b2b2b1.superficial epithelium：2.tunic albugine

4、a：dense supportingtissue3.primordial forllicle： A primary oocyte surrounded by a single layer of flattened follicular cells.The arrow shows the nucleus of the oocyte with delicate chromatin ,pale staining and a prominent nucleolus.123High magnification:Primary follicleThe black arrow shows the nucle

5、us of the oocyte with a prominent nucleolus located eccentrically.*shows multi-layered follicular cells-granulosal cells.The yellow arrow shows the theca folliculi.Secondary follicle at low magnification1.follicular antrum2.zona pellucida3.corona radiata： the single layer of tall columnar follicular

6、 cells attaching to the oocyte4.cumulus oophorus: the oocyte with the surrounding follicular cells project to the antrum5.zona granulosa：6a.theca interna6b.theca externa 123456a6b3Secondary follicle： (1)follicular antrum (2)corona radiata (3)cumulus oophorus (4)Call-Exner body A small round space su

7、rrounded by some follicular cells. (5)theca interna:The theca cells are polygonal in shape with round nuclei .(6)theca externa:(1)(2)(3)(4)(5)(6)1.atretic follicle：The degenerating primary follicle : left a wavy shaped zona pellucida ,red colour-glassy erstitial gland： formed by the de

8、generating secondary follicles:The theca cells enlarge and e polygonal shaped cells with acidophilic cytoplasm ,arranged in clusters.121developing corpus luteum:1.low magnification： collapsed follicular wall left a lumen with some red blood cells . 11High magnification： shows the granulosa lutein ce

9、lls,polygonal in shape,pale staining cytoplasm.1.advanced corpus luteum at low magnification：1a.shows the enveloped CT.11ahigh magnificationgranulosa lutein cells:Polygonal granulosa lutein cells ,acidphilic cytoplasm ,pale staining2b.theca lutein cells smaller ,densely staining not clear.high magni

10、ficationaa.granulosa lutein cells:Polygonal granulosa lutein cells ,acidphilic cytoplasm ,pale stainingb.theca lutein cells smaller ,densely staining.abG.granulosa lutein cells:Polygonal granulosa lutein cells ,the cytoplasm ,pale stainingT.theca lutein cells smaller ,densely staining. 46Section of

11、uterus：H-E stain the right aspect shows proliferative endometrium ,purple in color ;the broad intermediate, myometrium ;the left surface shows perimetrium black in color.This micrograph shows the endometrium in proliferative phase 1.the lining epithelium：Ciliated columnar epithelial cells and noncil

12、iated secretary cells. 2.laminar propria：2a.uterine gland： tubular gland,little tortuous .2b.stroma cells： the dotted structures between the glands are the nuclei of stroma cells.12a2b22aHigh magnification shows the proliferative phase of the endometrium .1.uterine gland：The glandular epithelium has

13、 a pseudostratified appearance2.spiral arteriole：Various planes can be seen.3.stroma cells: round or spindle shaped nuclei can be seen.123 47Section of uterus in secretory phase： The left aspect shows the endometrium ing thicker and pale staining , this is the endometrium in secretary phase .This mi

14、crograph shows the endometrium in secretory phase at low magnification.1.the lining epithelium：2.uterine gland: expending and more tortuous or saw-tooth appearance ,the basal vacuolations can not be seen.3.stroma :The stroma cells enlarge，pale staining cytoplasm.123high magnification:Secretary phase

15、 of the endometrium at late stage.1.the uterine glands enlarge greatly and contain secretion.2.cross section of spiral A.3.stroma cells :Polygonal ,pale staining cytoplasm. 1231uterine gland: at early stage :the tubular epithelial cells with basal vacuoles which is accumulating glycogen 48Section of

16、 the menstrual uterus：The purple-blue zone shows menstrual endometrium.Low power:Menstrual phase ofthe endometrium1.the shedding endometriumthe epithelium in the free surface has lost .2.stratum basalis： small uterine glands and packed stroma cells.3.myometrium:123Cross section of oviduct：H-E stain

17、These are hollow organs,because of a lot of folds at ampulla ,the lumen is not clear.High magnification：Ampulla of the oviduct1.mucosa 1a.epithelium： The ciliated columnar cells have round nuclei and pale staining cytoplasm.The secretary cells:Which are the densly staining cells. 1b.laminar propria： delicate connective tissue.2.muscular layer： inner circular and outer longitudinal layers of smooth muscle .3.serosa：covered by single layer of