生物信息學分析方法

1、常用的生物信息學分析方法第十組生物信息學是一門在生命科學的研究中，以計算機為工具對生物信息進行儲存、檢索和分析的科學。生物信息學Bioinformatics生物信息學基本上是分子生物學與信息技術的結合體。研究材料和結果是各種各樣的生物學數(shù)據(jù)研究工具是計算機研究方法包括對生物學數(shù)據(jù)的搜索（收集和篩選）、處理（編輯、整理、管理和顯示）及利用（計算、模擬）節(jié)律基因Timeless數(shù)據(jù)庫MGIhttp:/數(shù)據(jù)庫NCBI/Blast.cgi數(shù)據(jù)庫TAIR/http:/多序列比對MEGAhttp:/系統(tǒng)發(fā)育樹MEGAhttp:/tools/meme保守基序分析MEMEhttp:/基因結構GSDShttp:

2、/bioinformatics.psb.ugent.be/webtools/plantcare/html/啟動子分析PlantCARE基序名稱位置序列特征功能TATA-box254TATAAcore promoter elementCAAT-box260CCAATcommon cis-acting element in promoter and enhancer regions3-AF1 binding site50AAGAGATATTTlight responsive elementCCAAT-box1051CAACGGMYBHv1 binding siteCGTCA-motif1294CG

3、TCAMeJA-responsivenessGA-motif1252ATAGATAAlight responsive elementHSE28AAAAAATTTCheat stress responsivenessLTR414CCGAAAlow-temperature responsivenessMSA-like568CCCAACGGTcell cycle regulationTGA-element289AACGACauxin-responsive elementhttp:/轉錄因子結合位點分析JASPARMatrix IDNameScoreRelative scoreStartEndStra

4、ndPredicted sequenceMA0940.1AP112.570.9011628+acaaaaatgaaatMA0934.1AHL2512.51691328335+aattaattMA0951.1ATHB-1612.24230.964352359+taatgattMA0933.1AHL2012.0060.979328335+aattaattMA0943.1ARF511.84490.97410171024+GCCGACAGMA0947.1ARR1411.81320.965707714+AGATCCGCMA0934.1AHL2511.81260.984333340+atttaatthtt

5、p:/bar.utoronto.ca/可視化基因組學BARhttp:/suba.live亞細胞定位SUBAAGISUBAconPredictionsAT5G52910.1nucleusnucleuscytosolmitochondrionhttp:/www.sbg.bio.ic.ac.uk/phyre/三級結構預測Phyre16-464 replication643-747 protein bindinghttp:/www.cbs.dtu.dk/services/SignalP/信號肽預測SignalPAT5G52910AT1G01070http:/www.cbs.dtu.dk/service

6、s/TMHMM-2.0/跨膜結構域預測TMHMMhttp:/string.embl.de蛋白互作網(wǎng)絡STRINGDNA repairpolymerasehelicase序列分析雙序列比對MEGA/Bioedit/bioedit/bioedit.htmlNCBI/GenBank/MEME/tools/meme多序列比對（系統(tǒng)進化樹、保守基序）基因分析ORF(OpenReadingFrame)分析NBCI /orffinder/基因結構分析（外顯子、內含子）GSDS/啟動子分析PlantCAREhttp:/bioinformatics.psb.ugent.be/webtools/plantcare

7、/html/PLACEhttps:/sogo.dna.affrc.go.jp/cgi-bin/sogo.cgi?sid=&lang=en&pj=640&action=page&page=newplace轉錄因子結合位點分析TFSEARCHhttp:/diyhpl.us/bryan/irc/protocol-online/protocol-cache/TFSEARCH.htmlJASPAR/表達模式分析bar http:/bar.utoronto.ca/蛋白分析結構分析二級PredictProtein/PSIPREDhttp:/bioinf.cs.ucl.ac.uk/psipred三級Phyrehttp:/www.sbg.bio.ic.ac.uk/phyre/SWISS-MODEL/亞細胞定位PSORTSUBA http:/suba.live信號肽預測SignalPhttp:/www.cbs.dtu.dk/services/SignalP/跨膜結構預測TMHMMhttp:/www.cbs.dtu.dk/serv