COQ7-IN-1-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemECOQ7-IN-1Cat.No.:HY-133033CASNo.:2579696-72-1分?式:C??H??ClN?O分?量:271.7作?靶點:Others作?通路:Others儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:100mg/mL(368.05mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.6805mL18.4026mL36.8053mL5mM0.7361mL3.6805mL7.3611mL10mM0.3681mL1.8403mL3.6805mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(7.66mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性COQ7-IN-1?種?效的?輔酶Q(COQ7)抑制劑，可?擾泛醌(UQ)合成。COQ7-IN-1不會?擾正常培養(yǎng)細胞?長。COQ7-IN-1可?于研究UQ補充途徑(從頭合成UQ和胞外攝取UQ)之間的平衡[1]。體外研究COQ7-IN-1(Compound8;0-30μM;for4days)inhibitsWI-38cellsgrowthwithaGI50of19.0μM.COQ7-IN-1weaklyinhibitsC3AcellsgrowthwithGI50of9.0±1.1μM.COQ7-IN-1doesnotlimitC3Acellsurvivalthroughinhibitingaerobicrespirationorincreasingoxidativestress[1].COQ7convertsdemethoxy-UQ(DMQ)todemethyl-UQ(DMeQ),andthenconvertedtoUQbyCOQ3.CellsunderCOQ7deficiencyareunabletoproduceUQ,andconsequentlyaccumulateDMQ.DMQ10andUQ10contentsineachculturewellare13.2and12.2ng/wellforCOQ7-IN-1(10μM;for2days)culturedwithHeLacells.DMQ10contentofCOQ7-IN-1inHeLacellsis52.0%[1].CellViabilityAssay[1]CellLine:WI-38cellsConcentration:0-30μMIncubationTime:4daysResult:Theconcentrationthatcaused50%cellgrowthinhibition(GI50)was19.0μM.REFERENCES[1].KeikoTsuganezawa,etal.IdentificationofsmallmoleculeinhibitorsofhumanCOQ7.BioorgMedChem.2020Jan1;28(1):115182.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel: