QX77-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEQX77Cat.No.:HY-112483CASNo.:1798331-92-6分?式:C??H??ClN?O?分?量:300.74作?靶點(diǎn):Autophagy作?通路:Autophagy儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:15mg/mL(49.88mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.3251mL16.6257mL33.2513mL5mM0.6650mL3.3251mL6.6503mL10mM0.3325mL1.6626mL3.3251mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：50%PEG300>>50%salineSolubility:10mg/mL(33.25mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性QX77分?伴侶介導(dǎo)的?噬(CMA)激活劑，可上調(diào)LAMP2A的表達(dá)。QX77誘導(dǎo)Rab11上調(diào)，挽救Rab11下調(diào)和轉(zhuǎn)運(yùn)缺陷。QX77可抑制ES細(xì)胞?我更新，促進(jìn)分化。IC50&TargetCMA[1]體外研究QX77(48hours)upregulatesRab11expressionlevelsinCtns-/-MEFs[1].InCtns-/-MEFs,QX77recoversthedown-regulatedLAMP2Aexpression,andtheRab11-positivecarriervesiclesrecoverthehigh-motilitytraffickingphenotypeobserveinwild-typecells[1].TreatmentwithCMAactivatorQX77rescuesRab11down-regulationandtraffickingdeficiencyincystinoticcells.QX77treatmentalsoincreasesLAMP2Alocalizationatthelysosomalmembrane[1].QX77significantlyincreasesthere-localizationofLAMP2AatLAMP1-positivelysosomesincystinoticcell,itcorrectsthelocalizationofLAMP2Aatthelysosomalmembraneincystinoticcells[1].QX77protectscystinoticcellsfromtheincreasedsusceptibilitytotert-butyl-hydroperoxide-inducedoxidativestressandreconstitutestheresistantlevelsobservedinwild-typecells.TheeffectofQX77oncystinoticcellsurvivalisdependentonLAMP2Aexpression[1].QX77(10μM;0,3or6days)activatesCMAandincreasesLAMP2AexpressioninD3andE14EScell,itdownregulatespluripotencyfactorsandAPreactivityandpartiallylostthecharacteristicEScellmorphology[2].WesternBlotAnalysis[2]CellLine:ESD3cellline;E14TG2a(E14)celllineConcentration:10μMIncubationTime:3or6daysResult:IncreasedLAMP2AexpressionanddecreasedSOX2andOct4proteinexpression.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?JNeuroinflammation.2021Dec20;18(1):295.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].JinzhongZhang,etal.Cystinosin,thesmallGTPaseRab11,andtheRab7effectorRILPregulateintracellulartraffickingofthechaperone-mediatedautophagyreceptorLAMP2A.JBiolChem.2017Jun23;292(25):10328-10346[2].YiXu,etal.Chaperone-mediatedautophagyregulatesthepluripotencyofembryonicstemcells.Science.2020Jul24;369(6502):397-403.McePdfHeightCaution:Producthasnotbeenfullyvalidatedform