SMIFH2-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemESMIFH2Cat.No.:HY-16931CASNo.:340316-62-3分?式:C??H?BrN?O?S分?量:377.21作?靶點:Arp2/3Complex作?通路:Cytoskeleton儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:125mg/mL(331.38mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.6510mL13.2552mL26.5104mL5mM0.5302mL2.6510mL5.3021mL10mM0.2651mL1.3255mL2.6510mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.08mg/mL(5.51mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性SMIFH2?種formin特異性抑制劑。SMIFH2通過Formins抑制肌動蛋?聚合，并響肌動蛋?細胞?架[1]。IC50&TargetFormin[1]體外研究SMIFH2(25uM;1-16hours)inducesdynamiccytoskeletalremodellinginU2OScells[1].SMIFH2(25μM)reducesp300,mDia2andp53levelsinaproteasome-independentmanner[1].SMIFH2reducesexpressionandactivityofp53throughapost-transcriptional,proteasome-independentmechanismthatinfluencesremodellingofthecytoskeleton[1].WesternBlotAnalysis[1]CellLine:293T,A375,U2OS,andMDA-MB-231cellsConcentration:25μMIncubationTime:5hoursfor293T,U2OS,MDA-MB-231cells;2.5hoursforA375cellsResult:DownregulatedmDia2,p53andp300proteinlevels.REFERENCES[1].TadamotoIsogai,etal.SMIFH2haseffectsonForminsandp53thatperturbthecellcytoskeleton.SciRep.2015Apr30;5:9802.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-37