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分子生物學(xué)(MolecularBiology)知到章節(jié)測試答案智慧樹2023年最新山東第一醫(yī)科大學(xué)第一章測試

TheNucleicacidwillgetmaximumUVabsorbanceatwavelength_____()

參考答案:

260nm

ThecommoncomponentcomposedofDNAandRNAis_____()

參考答案:

Guanine

ThesecondarystructureofDNAis_____()

參考答案:

doublehelix

ThecorrespondinganticodontocodonACGis___()

參考答案:

CGU

SinglestrandDNA5’-CGGTA-3’canannealwiththefollowRNA_____()

參考答案:

5’-UACCG-3’

mRNAisusedas_____intranslation.()

參考答案:

template

AmountofTinRNAis0.25mol,soamountofAis_____.()

參考答案:

0.25mol

tRNAineukaryoticcellcontains_____()

參考答案:

5.8sRNA;5sRNA;18sRNA;28sRNA

ThebaseinDNA_____()

參考答案:

C;T;A;G

Stem-loopisatypeofsecondarystructurenotusuallyfoundintRNA.()

參考答案:

AllgenomesarecomposedofRNA.()

參考答案:

錯(cuò)

第二章測試

SequencesthatareremovedfromtheprimaryRNAtranscriptineukaryotesarecalled________.()

參考答案:

introns

Whichcharacterisnottrueforeukaryoticgenes?()

參考答案:

Alltheprotein-codinggenesinhighereukaryoteshaveintrons.

Whichisnotafeatureofenhancer?()

參考答案:

Ageneoftenhasonlyoneenhancer.

Theregulatorsofeukaryoticgeneexpressionconsistof________.()

參考答案:

enhancer;insulator;silencer

Allhousekeepinggeneshaveweakpromotorsandareregulatedtomaintaintheirgene

productsatconstantlowlevelconcentrations.()

參考答案:

錯(cuò)

第三章測試

BasedonMeselson-Stahlexperiment,eachdaughterDNAmoleculeiscomposedof()

參考答案:

oneoldandonenewstrand

Whichmechanismcanexplaintwostrandsaresynthesizedintheoppositedirection,inthereplicationfork?()

參考答案:

semidiscontinuousreplication

Althoughmammaliangenomeishuge,cellscanreplicatethegenomeinjustafewminutes.Whichisthemostpossiblereason?()

參考答案:

Hundredsofreplicationforksworksimultaneously

WhichisTUREabouttheinitiationofprokaryoticDNAreplication?()

參考答案:

DNAhelicaseunwindsDNAduplexatoriC

Whichis

FALSEabouttheterminationofprokaryoticDNAreplication?()

參考答案:

Coordinatethesynthesisoflaggingandleadingstrands

WhichisFALSEaboutDNApolIIIinprokaryotes?()

參考答案:

α,δ,ξsubunitsformcoreenzyme

MitochondrialDNAreplicatesin()

參考答案:

D-loopreplication

RNAprimerinDNAreplication()

參考答案:

isrequiredbyallOkazakifragments;provides3’-OHtosynthesizeDNA

DNAreplicationneeds()

參考答案:

helicase;primase;topoisomerase;DNAligase

WhatarethedifferencesofeukaryoticDNAreplicationfromprokaryotes?()

參考答案:

thereplicationoriginofeukaryotesisshorterthanthatinprokaryotes;telomereandtelomeraseaidterminationofeukaryotes;eukaryoticDNAisreplicatedinSphaseofcellcycle;eukaryoticDNAcontainsmultipleorigins

TheOkazakifragmentofeukaryotesislongerthanthatofprokaryotes.()

參考答案:

錯(cuò)

ReversetranscriptaseexertsthefunctionsofRNAdependentDNApolymeraseandDNAdependentDNApolymerase.()

參考答案:

第四章測試

WhichofthefollowingDNAdamagesbelongstoexogenousDNAdamage().

參考答案:

Ionizingradiation

WhichtypeofDNApointmutationswillnotleadtothechangeinaminoacid().

參考答案:

Silentmutation

whichbasesubstitutionbelowbelongsto“Transition”pointmutation.()

參考答案:

C→T

WhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair.()

參考答案:

null

InwhichprocessGG-NERandTC-NERshowdifferent()

參考答案:

DNAlesionrecognition

ThedeficiencyofNERrepairproteinsissusceptibleto_disease.()

參考答案:

Xerodermapigmentosum

Duringmismatchrepairinprokaryotes,thenewlysynthesizedDNAisrecognizedbythelackof()modification()

參考答案:

Methylation

DuringdoublestrandedDNAbreakrepair,NHEJshowsmoreprecisecomparedtoHR.()

參考答案:

錯(cuò)

DNAdamageisthemaincausefortheincreasedfrequencyofgenemutation.()

參考答案:

BaseexcisionrepairisoneofDNArepairpathwaystocorrectslargebaselesions.()

參考答案:

錯(cuò)

第五章測試

WhichcomponentoftheprokaryoteRNApolymeraseholoenzymeisinvolvedinrecognizingpromotersspecifictocertaintypesofgenes?()

參考答案:

σ

WhichRNApolymerasetranscribesthegeneofinsulin?()

參考答案:

RNApolymeraseII

AlltheRNAsbelowaretranscribedbyRNApolIexcept()

參考答案:

23SrRNA

Whichofthefollowingstepsoccursfirstinprokayotictranscription?()

參考答案:

formationofaclosedpromotercomplex

TheRhoproteinisinvolvedinthe_____stageoftranscription.()

參考答案:

termination

Inprokaryotes,RNApolymerasecanbindupstreamsequencesofpromotersdirectly.Eukaryotesneedsomeproteinstofacilitatethebinding.Whatarethenamesoftheseproteins?()

參考答案:

Transcriptionfactors

WhichtranscriptionfactorcanrecognizeandbindtoTATAbox?()

參考答案:

TFIID

AmutationtothissequenceineukaryoticmRNAwillaffecttheprocessbywhichthe3'-endpoly-AtailisaddedtothemRNA.()

參考答案:

AAUAAA

Thefunctionsof5’CapofeukaryoticmRNAinclude()

參考答案:

transportofthemRNAfromnucleustocytosol;;enhancementofthemRNA’stranslatability;;protectionofthemRNAfromdegradation;

Cis-actingelementsandtrans-actingfactorscontroltranscription.Thecis-actingelementsincludetheTATAbox,enhancersandotherelementsetal.()

參考答案:

RNAPolIIistheenzymesynthesizinghnRNAineukaryotes.Itisnotsusceptibletoamanitin.()

參考答案:

錯(cuò)

Eukaryotictranscriptionsisaccompaniedbytranslation.()

參考答案:

錯(cuò)

第六章測試

Weusuallycallcodonscontains5characteristics,amongwhichthedegeneracyofthegeneticcodemeans?()

參考答案:

Mostaminoacidshavemorethanonesetofcodons

AgenehasthebasesequenceACCCGTGCACGT.IftheAatthebeginningofthesequenceisdeleted,whatwillbehappen?()

參考答案:

AllofthecodonsafterApointwillbechanged

WhichbondisanaminoacidlinkedtothetRNA?()

參考答案:

ester

Theenergyrequiredforthestepofpeptidechainelongationinprokaryoticis?()

參考答案:

onlyGTP

TheShine-Dalgarnosequenceisfoundonthe________.()

參考答案:

mRNA

Amongthefollowingfactors,whichonecoulddelivertheaminoacyl-tRNAtotheAsiteofribosome?()

參考答案:

EF-Tu

ThespecificsequenceontheNterminusofsecretoryproteiniscalled()

參考答案:

signalpeptide

Whichproteinfactorhasthetranslocaseenzymeactivityineukaryotes:()

參考答案:

eEF-2proteinfactor

WhichoftheclassesofRNAmoleculesis/arepartoftheprocessof

translation?()

參考答案:

TransferRNA;MessengerRNA;RibosomalRNA

MessengerRNA(mRNA)ismadebyaprocesscalledtranslation.()

參考答案:

錯(cuò)

Polypeptidesunfoldinthecytoplasmofacelltoformafunctionalprotein.()

參考答案:

錯(cuò)

Thegeneticcodehasfourletters,A,T,G,andC.()

參考答案:

錯(cuò)

第七章測試

WhichisNOTafinalproductofgeneexpression?()

參考答案:

mRNA

Ifageneisexpressedatrelativelyconstantrateinalmostallthecells,itiscalleda(an)()

參考答案:

housekeepinggene

Themostefficientstepofgeneexpressionregulationis()

參考答案:

transcriptioninitiation

WhichoneisNOTacis-actingelement?()

參考答案:

activatorprotein

Theprokaryoticoperatorsequenceisusually().

參考答案:

locatedontheupstreamregionofpromoter

Intheregulationoflacoperon,whenthemediumcontainsbothglucoseandlactose,whichstatementistrue?()

參考答案:

bothrepressorandCAPareinactive

Inlacoperon,repressorproteincouldbindto()sequence.()

參考答案:

operator

Intheregulationoflacoperon,whenthemediumcontainsonlyglucose,whichstatementistrue?()

參考答案:

concentrationofcAMPislow,CAPisinactive

Ifageneisexpressedonlyincertainorgansortissues,thisiscalled()

參考答案:

spatialspecificityofgeneexpression

WhichisNOTthebasalcis-actingelementsineukaryotes?()

參考答案:

operator

Enhancersmustlocateontheupstreamregionofpromoter.()

參考答案:

錯(cuò)

Theactivechromatinusuallyshowslowlevelofmethylation.()

參考答案:

第八章測試

Whichstatementistrueaboutoncogenes?()

參考答案:

Theyareusuallyactivatedincancerdevelopment

Whichoneofthefollowingisoncogene?()

參考答案:

myc

Thefollowingchangespromoteoncogeneactivation,except()

參考答案:

Lossofheterozygosity

Themechanismsoftumorsuppressorgeneinactivationinclude()

參考答案:

Mutation;hypermethylation;Lossofheterozygosity

Inmostchronicmyelogenousleukemia,tumorigenesisiscausedbyoncogeneamplification()

參考答案:

錯(cuò)

第九章測試

ThebasicstepsofPCRreactionare:()

參考答案:

denaturation,annealing,extension

ThebasiccomponentsofthePCRsystemdoesnotinclude:()

參考答案:

NTP

WhichtechnologycanbeusedtoidentifyDNA?()

參考答案:

Southernblotting

Whicharerightaboutmolecularhybridization?()

參考答案:

FromtheunderstandingofDNAdenaturationandrenaturationphenomenon;Thehybridmoleculesmustbesingle

InthePCRreaction,thepurposeofannealingistomakethecombinationofprimerandtemplate.()

參考答案:

Proteinchiptechnologyhasthecharacteristicsoffastandhighthroughput,itcanbeusedtoanalyzethousandsofproteinsinthewholegenomelevelatthesametime.()

參考答案:

第十章測試

WhichofthefollowingstatementsisnottrueconcerningTypeIIrestrictionendonucleases?()

參考答案:

TheycancleavesingleDNA

Whichisnotnecessaryaspartofprokaryoticexpressionvector?()

參考答案:

PolyAsignal

Basedonthenamingrules,thefirstletterofarestrictionendonucleasemeans()

參考答案:

Bacterialgenus

Isocaudarnerpresents()

參考答案:

Variousrestrictionendonucleasesrecognizedifferentpalindromes,butproducesamestickyends

Plasmidsareusuallyintroducedintobacterialcellsby.()

參考答案:

Transformation

T4DNAligaserequires()

參考答案:

ATP

Ininsertionalinactivationscreening,thebacteriathataresensitivetotetracyclineandresistant

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