同尾酶匯總表

連接互相匹配的粘性末端，產(chǎn)生新的酶切位點(diǎn)通常連接互相匹配的粘性末端可以產(chǎn)生新的酶切位點(diǎn)。以下組合是通過計算機(jī)程序計算出來的，盡管我們希望能盡量保證其準(zhǔn)確性，但有些未經(jīng)實驗證實，因此不能保證100%的可行性。如果有同裂酶存在，則只列出其中的一個酶。本表中列出的內(nèi)切酶均為已商品化的內(nèi)切酶。識別序列不夠嚴(yán)謹(jǐn)?shù)膬?nèi)切酶（例如，識別多個序列的酶）只在括號內(nèi)標(biāo)出相關(guān)的序列，但注意這些酶不只切割該序列。"-"表示連接產(chǎn)物不能被再切割。酶連接到可進(jìn)行切割的酶Acc65IBanI(G/GTACC)Acc65I,BanI,KpnI,NlaIV,RsaI(G/GTACC)BsiWI,BsrGIRsaIAccI(GT/CGAC)AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--(GT/CGAC)ClaI,BstBI,TaqITaqIAciIAccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--(C/CGC)BsaHI(GR/CGCC),HinP1I,NarIAciIHpaIIHpaIIAclI(AA/CGTT)AccI(GT/CGAC),AciI,ClaI,BstBI,HinP1I,HpaII,NarI,TaqI--AflIII

(A/CGCGT)AscI,BssHIIBstUI,HhaI(A/CATGT)BspHI,NeoI,PciINlaIII(A/CGCGT)MluIAflIII,BstUI,MluIAgeI(A/CCGGT)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWI,BspEIBsaWI,HpaIIBsrFI(A/CCGGT),SgrAI(CA/CCGGTG)AgeI,BsaWI,BsrFI,HpaIINgoMIVBsrFI,HpaIIApaI(GGGCC/C)BanII(GGGCC/C),Bsp1286I(GGGCC/C)ApaI,BanII,Bsp120I,Bsp1286I,HaeIII,NlaIV,Sau96IApaLI(G/TGCAC)SfeI(C/TGCAG)BsgIApoI(A/AATTY)EcoRIApoI,Tsp509I(G/AATTY)EcoRIApoI,EcoRI,Tsp509I(R/AATTY)MfeI,Tsp509ITsp509IAscI(GG/CGCGCC)AflIII(A/CGCGT),MluIBstUI,HhaIBssHIIBssHII,BstUI,Cac8I,HhaIAseI(AT/TAAT)BfaI,Csp6I,NdeI--MseIMseIAsiSI(GCGAT/CGC)BsiEI(CGAT/CG)DpnII,PvuIPacIMseI

PvuIDpnII,PvuIAvaI(C/CCGGG)AgeI,BsaWI,BspEI,BsrFI(R/CCGGY),NgoMIV,SgrAI(CR/CCGGYG)HpaII,NeiI,SerFI(C/TCGAG)XhoIAvaI,TaqI,XhoI(C/TCGAG)SalITaqI(C/CCGGG)XmaIAvaI,BsaJI,HpaII,NeiI,SerFI,SmaIAvaII(G/GWCC)PpuMI(RG/GACCY)AvaII,NlaIV,Sau96IRsrIIAvaII,Sau96IPpuMI(RG/GTCCY)AvaII,BsmFI,NlaIV,Sau96IAvrII(C/CTAGG)NheI,SpeI,XbaIBfaIStyI(C/CTAGG)AvrII,BfaI,BsaJI,StyIBamHI(G/GATCC)BelI,DpnIIAlwI,DpnIIBglII,BstYI(R/GATCY)AlwI,BstYI,DpnIIBstYI(G/GATCC)AlwI,BamHI,BstYI,DpnII,NlaIVBanI(G/GTACC)Aee65IAee65I,BanI,KpnI,NlaIV,RsaI(G/GCGCC)KasIBanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIV(G/GTACC)BsiWI,BsrGIRsaIBanII

(GGGCC/C)ApaI,Bsp1286I(GGGCC/C)ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I(GAGCT/C)Bsp1286I(GAGCT/C),SaeIAluI,BanII,BsiHKAI,Bsp1286I,SacIBelI(T/GATCA)BamHI,BstYI(R/GATCY)AlwI,DpnIIBglII,MboIIDpnIIBfaI(C/TAG)AseI,Csp6I,MseI,NdeI--旳II(A/GATCT)BamHI,BstYI(R/GATCY)AlwI,BstYI,DpnIIBclI,DpnIIDpnIIBsaHI(GR/CGYC)AccI(GT/CGAC),ClaI,BstBI,TaqI--(GA/CGYC)AciI,HinP1IHgaI(GG/CGYC)AciI,HinP1IHhaI(GG/CGYC)HpaIIAciI(GA/CGYC)NarIBsaHI,HgaI(GG/CGYC)NarIBanI,BsaHI,HaeII,HhaI,NarI,NlaIVBsaWI(W/CCGGW)AgeI,BsrFI(R/CCGGY),SgrAI(CR/CCGGYG)AgeI,BsaWI,BsrFI,HpaIIAvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBspEIBsaWI,BspEI,HpaIIBsrFI(R/CCGGY),NgoMIVBsrFI,HpaIINgoMIVHpaII

BsiEI(CGAT/CG)PacIMseI(CGAT/CG)PvuIBsiEI,DpnII,PvuI(CGGC/CG)SacIIAciIBsiHKAI(GTGCA/C)Bsp1286I(GTGCA/C)BsiHKAI,Bsp1286IBsp1286I(GAGCA/C)BsiHKAI,Bsp1286IBsp1286I(GAGCT/C),SacIAluI,BanII,BsiHKAI,Bsp1286I,SacINsiI--PstI,SbfIBsgIBsiWI(C/GTACG)Acc65I,BanI(G/GTACC),BsrGIRsaIBsp1286I(GGGCC/C)ApaI,BanII(GGGCC/C)ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I(GTGCA/C)BsiHKAIApaLI,BsiHKAI,Bsp1286I(GGGCC/C)BanII(GGGCC/C)BanII,Bsp1286I(GAGCT/C)BanII(GAGCT/C),BsiHKAI,SacIAluI,BanII,BsiHKAI,Bsp1286I,SacI(GWGCW/C)BsiHKAIBsiHKAI,Bsp1286I(GTGCA/C)NsiI--(GTGCA/C)PstI,SbfIBsgIBspEIAgeI,BsaWI,BsrFI(R/CCGGY),SgrAI(CR/CCGGYG)BsaWI,HpaII

(T/CCGGA)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWIBsaWI,BspEI,HpaIIBsrFI(R/CCGGY),NgoMIVHpaIIBspHI(T/CATGA)AflIII(A/CATGT),NcoI,StyI(C/CATGG)NlaIIIBsrFI(A/CCGGY)AgeI,BsaWIAgeI,BsaWI,BsrFI,HpaII(G/CCGGY)AgeI,BsaWI,NgoMIVBsrFI,HpaII(R/CCGGY)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFI(A/CCGGY)BsaWI,BspEIBsaWI,HpaII(R/CCGGY)BsaWI,BspEIHpaII(G/CCGGY)NgoMIVBsrFI,Cac8I,HpaII,NaeI(CR/CCGGYG)SgrAIBsrFI,HpaIIBsrGI(T/GTACA)Acc65I,BanI(G/GTACC),BsiWIRsaIBssHIIAflIII(A/CGCGT),MluIBstUI,HhaI(G/CGCGC)AscIBssHII,BstUI,Cac8I,HhaIBstBIAccI(GT/CGAC),ClaI,TaqITaqI(TT/CGAA)AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--BstYI(A/GATCY)BamHI,BglIIAlwI,BstYI,DpnII

(G/GATCY)BamHIAlwI,BamHI,BstYI,DpnII,NlaIV(R/GATCY)BelI,DpnIIDpnII(G/GATCY)BelI,DpnIIAlwI,DpnII(A/GATCY)BglIIBglII,BstYI,DpnIIClaI(AT/CGAT)AeeI(GT/CGAC),BstBI,TaqITaqIAeiI,AelI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--Dpnll/MboI/Sau3AI(/GATC)BamHI,BstYI(R/GATCC)AlwI,DpnIIBelI,BglII,BstYI(R/GATCY)DpnIIEaeI(Y/GGCCR)PspOMIHaeIII,Sau96I(C/GGCCR)EagIBsiEI,EaeI,EagI,HaeIII(T/GGCCR)EagIEaeI,HaeIII(C/GGCCR)NotIAeiI,BsiEI,EaeI,EagI,Fnu4HI,HaeIII(T/GGCCR)NotIAeiI,EaeI,Fnu4HI,HaeIIIEagI(C/GGCCG)PspOMIHaeIII,Sau96IEaeI(Y/GGCCR)EaeI,HaeIIIEaeI(C/GGCCG)BsiEI,EaeI,EagI,HaeIIINotIAeiI,BsiEI,EaeI,EagI,Fnu4HI,HaeIIIEcoRI(G/AATTC)ApoI(G/AATTC)ApoI,EeoRI,Tsp509IApoI(R/AATTY)ApoI,Tsp509I

MfeI,Tsp509ITsp509IHinP1I(G/CGC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--AciI,BsaHI(GR/CGCC),NarIHhaIBsaHI(GR/CGTC)HgaIHpaIIAciIHpall/MspI(C/CGG)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--AciI,BsaHI(GR/CGCC),HinP1I,NarIAciIKasI(G/GCGCC)Ban1(G/GCGCC)BanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIVMfeI(C/AATTG)ApoI(R/ATTTY),EcoRI,Tsp509ITsp509IMluI(A/CGCGT)AflIII(A/CGCGT)AflIII,BstUI,MluIAscI,BssHIIBstUI,HhaIMseI(T/TAA)AseIMseIBfaI,Csp6I,NdeI--NarI(GG/CGCC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--AciI,HinP1IHhaIBsaHI(GR/CGCC)BanI,BsaHI,HaeII,HhaI,NarI,NlaIVBsaHI(GR/CGTC)BsaHI,HgaIHpaIIAciINeoIAflIII(A/CATGT),BspHINlaIII

(C/CATGG)StyI(C/CATGG)BsaJI,NcoI,NlaIII,StyIPciINlaIIINdeI(CA/TATG)AseI,BfaI,Csp6I,MseI--NgoMIV(G/CCGGC)AgeI,BsaWI,BsrFI(R/CCGGY),SgrAIBsrFI,HpaIIAvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWI,BspEIHpaIIBsrFI(R/CCGGC),SgrAIBsrFI,Cac8I,HpaII,NaeINheI(G/CTAGC)AvrII,SpeI,StyI(C/CTAGG),XbaIBfaINlaIII(CATG/)SphI,NspINlaIIINotI(GC/GGCCGC)PspOMIAciI,EaeI,Fnu4HI,HaeIIIEagIAciI,BsiEI,EaeI,EagI,Fnu4HI,HaeIIIEaeI(Y/GGCCR)AciI,BsiEI,EaeI,Fnu4HI,HaeIIINsiI(ATGCA/T)BsiHKAI(GTGCA/C),Bsp1286I(GTGCA/C),PstI,SbfI--NspI(RCATG/Y)NlaIII,SphINlaIIIPacI(TTAAT/TAA)BsiEI(CGAT/CG),PvuIMseI

PciI(A/CATGT)AflII(A/CATGT),BspHI,NcoI,StyI(C/CTAGG)NlaIIIPpuMI(RG/GWCCY)AvaII,RsrIIAvaII,Sau96I(GG/GTCCY)AvaII,RsrIIAvaII,BsmFI,NlaIV,Sau96I(GG/GACCY)AvaII,RsrIIAvaII,NlaIV,Sau96IPspOMI(G/GGCCC)EaeI(Y/GGCCR),EagIHaeIII,Sau96INotIAciI,Fnu4HI,HaeIII,Sau96IPstI(CTGCA/G)BsiHKAI,Bsp1286I(GTGCA/C)BsgINsiI--SbfIPstIPvuI(CGAT/CG)AsiSIDpnI,PvuIPacIMseIBsiEI(CGAT/CG)BsiEI,DpnII,PvuIRsrII(CG/GWCCG)AvaII,PpuMI(RG/GACCY)AvaII,Sau96IPpuMI(RG/GACCY)AvaII,NlaIV,Sau96IPpuMI(RG/GTCCY)AvaII,BsmFI,NlaIV,Sau96ISacI(GAGCT/C)BanII(GAGCT/C),BsiHKAI,Bsp1286I(GAGCT/C)AluI,BanII,BsiHKAI,Bsp1286I,SacISacII(CCGC/GG)BsiEI