一株基于高通量測(cè)序降解亞硝酸鹽芽孢桿菌的篩選

本研究旨在通過(guò)高通量測(cè)序?qū)喯跛猁}芽孢桿菌（Bacillusnitrite）ThisstudyaimstoscreenforBacillusnitrite,abacteriumdegradesnitrite,usinghigh-throughputsequencing.Ammonia,nitrite,andnitricacidarethemainpollutantsinaquaticenvironments.Bacillusnitriteisabacteriumthatusesnitritetodegradeammoniaandnitrite.InordertodeterminethedegradationcharacteristicsofBacillusnitriteandthecorrespondingscreeningconditions,itsgrowthconditionsanddegradationproductswerestudiedindepth.Throughtheconstructionofadegradationscreeningmethodbasedonhigh-throughputsequencing,amoreefficientstrainofBacillusnitritewasscreened,anditsstrainidentificationandgenomesequencingwereanalyzed.Keywords:high-throughputsequencing;Bacillusnitrite;degradationscreening;genomesequencing;identificationBacillusnitrite16SrRNABLAST分析LB20選取適當(dāng)?shù)膩喯跛猁}濃度，將菌株接入到含有亞硝酸鹽的培養(yǎng)基中。通過(guò)紫外分光光度計(jì)測(cè)定亞硝酸鹽濃度的變化，并對(duì)降解產(chǎn)物進(jìn)行分析。DNAIlluminaHiseq測(cè)序完成基因DNA的測(cè)序。對(duì)測(cè)序結(jié)果進(jìn)行分析和注釋?zhuān)?duì)篩選的效率較高的菌16SrRNABLAST軟件分析菌Bacillusnitrite16SrRNA404℃兩種1g/L亞硝酸鹽的降解率達(dá)到70以上，并在降解過(guò)程中逐漸將亞硝酸鹽轉(zhuǎn)化為氮?dú)?。產(chǎn)物分析表明，在降解過(guò)程中，產(chǎn)生大量氨、氮?dú)獾然衔?，其產(chǎn)物釋放速率隨亞硝酸Bacillusnitrite菌株一致的關(guān)鍵代謝途徑和生物體功能基因，表明篩選成功的菌株與其他Bacillusnitrite菌株基因在結(jié)構(gòu)上具有較高的相似性。本