富鐵酵母的資源化利用

第第#頁(yè)共7頁(yè)細(xì)胞鐵含量的測(cè)定采用鄰二氮菲比色法測(cè)定[27]。細(xì)胞總鐵含量(mgFePL)=細(xì)胞生物量(gPL)@細(xì)胞鐵含量(mgFePg菌體干重)3富鐵酵母的應(yīng)用前景缺鐵性貧血是遍及全球的營(yíng)養(yǎng)缺乏癥,特別是發(fā)展中國(guó)家尤為普遍,富鐵酵母的食用將減少人類鐵缺乏癥的困擾,起到一定的預(yù)防疾病的作用。富鐵酵母具有穩(wěn)定性好,吸收率高、抗干擾、與食品中其他成分協(xié)同配合性好,并具有良好風(fēng)味等優(yōu)點(diǎn)。隨著對(duì)富鐵酵母基礎(chǔ)和應(yīng)用的研究,其應(yīng)用前景會(huì)更廣泛。參考文獻(xiàn):翁聰穎，劉啟沛，徐達(dá)道?高鐵食用酵母鐵資源生物利用率評(píng)價(jià).N、大鼠預(yù)防性評(píng)價(jià)[J].營(yíng)養(yǎng)學(xué)報(bào),1989,11(2):311-317.劉啟沛，翁聰穎，徐達(dá)道?高鐵食用酵母鐵資源生物利用率評(píng)價(jià).0、嬰幼兒喂養(yǎng)試驗(yàn)[J].營(yíng)養(yǎng)學(xué)報(bào)，1990,12(2):172-177.王偉利，錢愛(ài)東,林艷春，等.酵母富集微量元素鐵的研究[J].中國(guó)微生態(tài)學(xué)雜志,2002,14(2):83-84.袁玉蘭，郭雪娜，張博潤(rùn)，等.高生物量富鐵酵母菌的選育及其發(fā)酵條件的研究[J].工業(yè)微生物，2004，34(4):29-33.張培茵，閻喜霜,姜淑梅，等.鐵、鋅對(duì)啤酒酵母生物特性的影響研究[J].食品工業(yè)科技,1996,93(1):12-15.石長(zhǎng)波，閻喜霜,姜淑梅，等.高鐵食用酵母的研究[J].食品與發(fā)酵工業(yè)，1996,12(1):39-40.GaudreauH，TompkinsTA，ChampagneCP.Thedistributionofironiniron-enrichedcellsofSaccharomycescerevisiae[J].ActaAlimentaria，2001，30(4):355-361.SchilkeB，VoisineC，BeinertH，etal.EvidenceforaconservedsystemforironmetabolisminthemitochondriaofSaccha-romycescerevisiae[J].Proc.Natl.Acad.Sci.USA，1999，96(19):10206-10211.LiangtaoLi，ChenOS，WardDM，etal.CCC1isatransporterthatmediatesvacuolarironstorageinyeast[J].J.Biol.Chem，2001，276(31):29515-29519.SzczypkaMS，ZhuZ，SilarP，etal.Saccharomycescerevisiaemutantsalteredinvacuolefunctionaredefectiveincopperdetoxificationandiron-responsivegenetranscription[J].Yeast，1997，13(15):1423-1435.BodeHP，DumschatM，GarottiS，etal.Ironsequestrationbytheyeastvacuole-AstudywithvacuolarmutantsofSaccha-romycescerevisiae[J].Eur.J.Biochem，1995，228(2):337-342.RaguzziF，LesuisseMandCrichtonRR.IronstorageinSac-charomycescerevisiae[J].FEBSLetter,1988,231(1):253-258.[13]UrbanowskiJLandPiperRC.TheirontransporterFth1pformsacomplexwiththeFetsironoxidaseandresidesonthevacuolarmembrane[J].J.Biol.Chem,1999,274(53):38061-38070.DancisA,RomanDG,AndersonGJ,etal.FerricreductaseofSaccharomycescerevisiae:Molecularcharacterization,roleinironuptake,andtranscriptionalcontrolbyiron[J].Proc.Natl.Acad.Sci.USA,1992,89(9):3869-3873.EideD,Davis-KaplanS,JordanI,etal.Regulationofironup-takeinSaccharomycescerevisiae[J].J.Biol.Chem,1992,267(29-30):20774-20781.DixDR,JamieBT,MargaretBA,etal.TheFET4geneen-codesthelowaffinityFe(II)transportproteinofSaccha-romycescerevisiae[J].J.Biol.Chem,1994,269(42):26092-26099.[17]GeorgatsouEandAlexandrakiD.Twodistinctlyregulatedgenesarerequiredforferricreduction,thefirststepofironup-takeinSaccharomycescerevisiae[J].MolecularandCellularBiology,1994,14(5):3065-3073.StearmanR,YuanDS,Yamaguchi-IwaiY,etal.Apermease-oxidasecomplexinvolvedinhigh-affinityironuptakeinyeast[J].Science,1996,271(15):1552-1557.DeSilvaDM,AskwithCC,EideD,etal.TheFET3geneproductrequiredforhighaffinityirontransportinyeastisacellsurfaceferroxidase[J].J.Biol.Chem,1995,270(3):1098-1101.DancisA,YuanDA,HaileD,etal.MolecularcharacterizationofacoppertransportproteininSaccharomycescerevisiae:Anunexpectedroleforcopperinirontransport[J].Cell,1994a,76(2):393-402.FuD,BeelerTJ,andDunnTM.Sequence,MappingandDis-ruptionofCCC2,agenethatcross-complementstheCa2+-sen-sitivephenotypeofcsg1mutantsandencodesap-typeATPasebelongingtotheCu2+-ATPasesubfamily[J].Yeast,1995,11(3):283-292.LiangtaoLiandKaplanJ.Characterizationoftwohomologousyeastgenesthatencodemitochondrialirontransporters[J].J.Biol.Chem,1997,272(45):28485-28493.Yamaguchi-IwaiY,DancisAandKlausnerRD.AFT1:amedi-atorofironregulatedtranscriptionalcontrolinSaccharomycescerevisiae[J].EMBOJ.,1995,14(6):1231-1239.56[24]賈盤(pán)興,蔡金科,馬德欽,等.微生物遺傳學(xué)實(shí)驗(yàn)技術(shù),北京:科學(xué)出版社,1992,259諸葛健,王正祥.工業(yè)微