多聚賴氨酸包被方法

多聚賴氨酸包被方法CCB-BUUT-202108]Companynumber多聚賴氨酸包被方法CCB-BUUT-202108]Companynumber: [0089WT-889U!1.Tocoatcoverslips:Prepareastocksolutionbydissolving25mg/mlpolylysineinmlwater(bothpoly-L-lysineandpoly-D-lysineareusedtocoattissueculturesurfaces;checkspecificprotocolforchoiceofisomer)andfiltersterilizethroughapmfilter.Storein100-plaliquotsat-20°C.Whenreadytouse,diluteonealiquotin40mlwatertoprepare13pg/mlworkingsolution.Sterilizecoverslipsbyautoclavingpriortocoating.Dipcoverslipsintheworkingsolution,thenincubate15mintoseveralhoursinahumidified37°C,5%CO2incubator.Allowsurfacetodry.2.Tocoatculturedishes:多聚賴氨酸包被培養(yǎng)皿/板Prepareastocksolutionbydissolving100mgpoly-lysinein100mlwater(bothpoly-L-lysineandpoly-D-lysineareusedtocoattissueculturesurfaces;checkspecificprotocolforchoiceofisomer)andfiltersterilizethroughapmfilter.配制1mg/ml儲(chǔ)存液Storein5-mlaliquotsat-20°C.分裝保存在-20度Whenreadytouse,dilute1partstocksolutionwith9partswatertoprepare100pg/mlworkingsolution.使用前按1：10稀釋成100pg/ml工作濃度。用5-10ml一次性無(wú)菌注射器和pm濾器過(guò)濾除菌，備用。Filltissueculturedishesorslidewellswiththeworkingsolutionandincubate1hrinahumidified37°C,5%CO2incubator,thenremovesolutionbyvacuumaspirationandallowsurfacetodry.加入培養(yǎng)皿或培養(yǎng)板中，使能完全覆蓋皿底即可，37度培養(yǎng)箱放置1小時(shí)或置超凈臺(tái)內(nèi)室溫過(guò)夜。然后吸取液體，使表面晾干。Storecoatedtissueculturewareupto3monthsat4°C.可以立即使用也可保存在4度3個(gè)月備用。Usedilutedsolutionsonlyonce,butunuse