抗氧化青蘋果醋與葡萄多酚對(duì)小鼠尿酸血癥的預(yù)防作用

抗氧化物蘋果醋與葡萄多酚對(duì)小鼠高尿酸血癥的預(yù)防作用指導(dǎo)老師：林明棟、余劍平組員：劉嘉偉、張劍威、程慧華、漆微韡、李鵬亮——05物理小組前言

高尿酸與痛風(fēng)

高尿酸的治療

本實(shí)驗(yàn)的方向

蘋果醋與葡萄多酚

材料與方法材料與方法材料與方法實(shí)驗(yàn)記錄組別性別原始編號(hào)分組日體重取血日體重灌胃時(shí)間造模時(shí)間注射肝素時(shí)間取血時(shí)間組內(nèi)編號(hào)性別原始編號(hào)分組日體重取血日體重灌胃時(shí)間造模時(shí)間注射肝素時(shí)間取血時(shí)間組內(nèi)編號(hào)空白雄12930.2——8:559:001雌128.927.9——9:459:5010193233.1——9:059:102253131.3——9:5510:1092433.233.5——9:159:203223.926.5——10:0510:3082034.334.5——9:259:3042429.830——10:1510:5071234.935.5——9:359:4052028.229.3——10:2511:106造模629.230.6—19:1020:0520:106233032—19:0019:5520:0051430.333—19:2020:1520:2072927.527.6—18:5019:4519:5042933.336.6—19:3020:2520:30892730.5—18:4019:3519:4031534.436.8—19:4020:3520:409728.929.6—18:3019:2519:3022635.636.2—19:5020:4520:50101132.334.3—18:2019:1519:2012021年6月18日實(shí)驗(yàn)記錄組別性別原始編號(hào)分組日體重取血日體重灌胃時(shí)間造模時(shí)間注射肝素時(shí)間取血時(shí)間組內(nèi)編號(hào)性別原始編號(hào)分組日體重取血日體重灌胃時(shí)間造模時(shí)間注射肝素時(shí)間取血時(shí)間組內(nèi)編號(hào)高蘋果醋雄273034.813:0014:0014:5515:001雌2727.629.813:5014:5015:4515:5012530.432.813:1014:1015:0515:102302727.314:0015:0015:5516:002233233.513:2014:2015:1515:203430.231.514:1015:1016:0516:103934.536.313:3014:3015:2515:304528.328.714:2015:2016:1516:204735.838.313:4014:4015:3515:4052633.830.514:3015:3016:2516:305低蘋果醋30303115:3016:3017:2517:306222929.514:4015:4016:3516:406283136.315:4016:4017:3517:4071027.829.314:5015:5016:4516:507232.333.615:5016:5017:4517:508827.128.215:0016:0016:5517:0081333.536.216:0017:0017:5518:009213433.215:1016:1017:0517:109113634.816:1017:1018:0518:10101728.52715:2016:2017:1517:20102021年6月19日實(shí)驗(yàn)記錄組別性別原始編號(hào)分組日體重取血日體重灌胃時(shí)間造模時(shí)間注射肝素時(shí)間取血時(shí)間組內(nèi)編號(hào)性別原始編號(hào)分組日體重取血日體重灌胃時(shí)間造模時(shí)間注射肝素時(shí)間取血時(shí)間組內(nèi)編號(hào)高多酚雄223132.19:5510:5511:5011:551雌628.128.49:3010:3011:2511:3011833.335.310:0011:0011:550:0022828.528.79:3510:3511:3011:352434.235.910:0511:0512:0012:053183030.39:4010:4011:3511:40353539.410:1011:1012:050:1041931.9289:4510:4511:4011:4541634.534.810:1511:1512:1012:1551232.231.39:5010:5011:4511:505低多酚1025.42510:2011:2012:150:2061426.627.410:4511:4512:4012:456332.529.110:2511:2512:2012:257328.327.810:5011:5012:450:507833.536.310:3011:3012:250:3081329.229.810:5511:5512:5012:5582134.837.510:3511:3512:3012:359153030.411:0012:0012:551:0091734.535.110:4011:4012:350:4010163233.711:0512:0513:0013:05102021年6月20日實(shí)驗(yàn)結(jié)果性別空白組測定結(jié)果造模組結(jié)果高蘋果醋組結(jié)果低蘋果醋組結(jié)果高多酚組結(jié)果低多酚組結(jié)果組內(nèi)編號(hào)尿酸μmol/LMDAnmol/mL組內(nèi)編號(hào)尿酸μmol/LMDAnmol/mL組內(nèi)編號(hào)尿酸μmol/LMDAnmol/mL組內(nèi)編號(hào)尿酸μmol/LMDAnmol/mL組內(nèi)編號(hào)尿酸μmol/LMDAnmol/mL組內(nèi)編號(hào)尿酸μmol/LMDAnmol/mL雄1121.737.710940.716.41712.619.010622.912.01505.016.110875.411.92114.946.79827.516.22404.021.09707.011.52704.219.19939.916.43121.740.58934.214.43670.620.58723.913.83698.616.48956.716.34101.439.57927.816.44572.417.37782.812.44726.719.77673.412.85128.436.96927.815.15448.919.26751.915.35620.112.56925.911.9雌6118.337.95931.013.86566.718.65101514.76704.214.45920.313.17114.946.94850.212.07552.717.54886.613.37676.218.64892.215.28138.640.73908.412.08477.021.73760.312.38718.212.73684.615.89114.936.22685.310.39735.116.12746.313.69648.117.22715.412.310158.834.31840.511.810561.118.11998.811.210454.516.61667.713.4原始數(shù)據(jù)實(shí)驗(yàn)結(jié)果GroupUricacidμmol/LMDAnmol/mL空白123.35±15.7939.72±4.21造模877.33±80.2a13.84±2.22a高蘋果醋570.11±110.04b18.90±1.77a低蘋果醋799.61±127.55c13.02±1.36a高葡萄多酚645.58±93.93b16.31±2.51a低葡萄多酚825.15±123.06c13.91±1.81aa.與空白組相比，P<0.01b.與造模組相比，P<0.01c.與造模組相比，P>0.05兩組間比較采用獨(dú)立樣本t檢驗(yàn)實(shí)驗(yàn)結(jié)果實(shí)驗(yàn)結(jié)果討論討論參考文獻(xiàn)[1]徐立，時(shí)樂.小鼠代謝性高尿酸血癥模型的復(fù)制方法初探.中國比較醫(yī)雜志20061:1[2]金沈銳，鄭軍.小鼠高尿酸血癥模型初探成都中醫(yī)藥大學(xué)學(xué)報(bào)199922(1):49-52[3]金瑩，孫愛東.蘋果多酚對(duì)小鼠的抗氧化作用的研究中國食物與營養(yǎng)20061:31-33[4]王海東，葛飛.金錢