GB/T 44349-2024蜂花粉總多酚的檢測福林酚試劑比色法

ICS

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CCS

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中華人民共和國國家標準

GB/T44349—2024

蜂花粉總多酚的檢測福林酚試劑

比色法

Detectionoftotalpolyphenolsinbeepollen—Colorimetricmethodusing

Folin-Ciocalteureagent

2024-09-29發(fā)布2025-04-01實施

國家市場監(jiān)督管理總局發(fā)布

國家標準化管理委員會

GB/T44349—2024

目次

前言

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范圍

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規(guī)范性引用文件

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術語和定義

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原理

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試劑和材料

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儀器設備

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分析步驟

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7.1

試樣制備

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7.2

試樣前處理

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7.3

空白試驗

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7.4

標準曲線的制作

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7.5

試樣測定

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結果計算

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定量限

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精密度

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附錄A（資料性）

福林酚試劑（Folin?Ciocalteureagent）配制方法

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參考文獻

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Ⅰ

GB/T44349—2024

前言

本文件按照GB/T1.1—2020《標準化工作導則第1部分：標準化文件的結構和起草規(guī)則》的規(guī)

定起草。

請注意本文件的某些內容可能涉及專利。本文件的發(fā)布機構不承擔識別專利的責任。

本文件由中華全國供銷合作總社提出。

本文件由全國蜂產品標準化技術委員會（SAC/TC601）歸口。

本文件起草單位：中國計量大學、安徽省王巢食品有限公司、廣東正當年生物科技股份有限公司、

中科標準（寧德）科技有限公司、?？萍瘓F（廈門）股份有限公司、深圳職業(yè)技術大學、秦皇島海關技

術中心、阜陽職業(yè)技術學院、南京眾譜生物科技有限公司、浙江省農業(yè)科學院、浙江省中醫(yī)藥研究院、

中國農業(yè)科學院蜜蜂研究所、青海省花寶蜂業(yè)股份合作公司、杭州天廚蜜源保健品有限公司、蘭溪市產

品商品質量檢測研究院、山東世通檢測評價技術服務有限公司、陜西國蜂大健康產業(yè)有限公司、江西汪

氏蜜蜂園有限公司。

本文件主要起草人：李紅亮、胡童霞、岳振峰、吳帆、崔宗巖、徐錦忠、曹聯(lián)飛、韓亞超、

張敬群、王磊、趙光培、蔣先志、陳璇、李強強、董捷、李平、林影、李斌、車延年、陳云、汪玲。

Ⅲ

GB/T44349—2024

蜂花粉總多酚的檢測福林酚試劑

比色法

1范圍

本文件描述了測定蜂花粉中總多酚的福林酚試劑比色方法。

本文件適用于蜂花粉中總多酚含量的測定。

2規(guī)范性引用文件

下列文件中的內容通過文中的規(guī)范性引用而構成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對應的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB5009.3—2016食品安全國家標準食品中水分的測定

GB/T6682分析實驗室用水規(guī)格和試驗方法。

3術語和定義

下列術語和定義適用于本文件。

3.1

蜂花粉beepollen

工蜂采集花粉，用唾液和花蜜混合后形成的物質。

[來源：GB/T30359—2021，3.2]

4原理

蜂花粉經75%乙醇溶液超聲提取的總多酚物質的酚羥基在堿性條件下，與福林酚試劑中磷鎢鉬酸的

六價鎢離子（W6+）反應生成藍色的五價鎢離子（W5+），可在765nm波長處產生最大吸收。在一定濃

度范圍內，其吸光度與總多酚類化合物含量成正比。與沒食子酸標準曲線比較，可定量測定總多酚化合

物的含量。

5試劑和材料

除非另有規(guī)定，僅使用分析純試劑。

5.1水，GB/T6682，三級。

5.2無水乙醇。

5.3無水碳酸鈉。

5.4福林酚試劑（Folin?Ciocalteureagent）：1mol/L（或1N），以棕色瓶分裝，4℃避光保存。直

接購買或配制，配制方法見附錄A。

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