醫(yī)學教材 丙型肝炎病毒(基礎)

丙型肝炎病毒邢文革

2014-12-17石家莊丙型肝炎病毒的發(fā)現(xiàn)1970’s:HepatitisAandBdiagnosticsMosttransfusionassociatedhepatitisnotcausedbyHAVandHBVorotherknownviralagents(NEJM292:767;Gastro.69:1278;JID139:511;VoxSang.44:231)non-A,non-Bhepatitis(NANBH)Mightbeasmall,envelopedvirustransmissibletochimpanzees(Gastro88:773;JID156:636)StandardimmunologicalmethodsfailedtoidentifyviralantibodiesorantigensChirongroupThoughtthattherewasnotenoughviralantigeninNANBHSotheydecidedtotryandconcentratethevirus丙型肝炎病毒的發(fā)現(xiàn)1.傳染性肝炎(HA)血清型肝炎(HB)2.動物模型（黑猩猩）3.基因文庫4.放射免疫標記5.全基因測序HightiterNANBHchimpanzeeplasmaUltracentrifugationat104,000gfor5hoursExtractnucleiacid,randomprimedcDNAintogt11phageImmunoscreenedwithNANBHpatientserumClone5-1-1LargeroverlappingClone81丙型肝炎病毒的發(fā)現(xiàn)PhagewithportionsofHCVgenomeInfectE.coliPhagekillsE.coliandleavesplaquesScreenwithHCVPatientserumLibraryScreening丙型肝炎病毒的發(fā)現(xiàn)WesternblotClone5-1-1expressedasSODfusionproteincalledPS5PS5SODNANBHpatientserumExperimentallyinfectedchimpserumProteinfromclone5-1-1closelyassociatedwithNANBHinfectionsHCVwasthefirstvirustobeisolatedandcharacterizedsolelybymolecularmethods,iewithoutculturemethods黑猩猩易感HCV病原學黃病毒科（Flaviviridae）丙型肝炎病毒屬包膜糖蛋白包膜+ssRNA核衣殼HepatitisCGenome

RegionsCodingforStructural&Non-StructuralProteins5′Non-codingRegion3′Non-codingRegionGenesCodingfor

StructuralProteinsGenesCodingfor

Non-Structural(NS)ProteinsHCVGenomeHepatitisCVirus

TranslationHCVGenomeC

ANS2NS3E1p7E2

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