分子生物學知到智慧樹章節(jié)測試課后答案2024年秋中南大學

分子生物學知到智慧樹章節(jié)測試課后答案2024年秋中南大學第一章單元測試

核苷中糖分別與嘧啶和嘌呤的哪個位點結(jié)合？（）。

A:嘧啶的1號位和嘌呤的9號位B:嘧啶的1號位和嘌呤的1號位C:嘧啶的9號位和嘌呤的1號位D:嘧啶的9號位和嘌呤的9號位

答案:嘧啶的1號位和嘌呤的9號位在DNA或RNA合成中，三磷酸核苷除了一個磷酸基團進入新合成的核酸鏈以外，另外兩個磷酸以（）形式離開。

A:焦磷酸B:與蛋白質(zhì)結(jié)合C:都不對D:單個磷酸分子

答案:焦磷酸在真核生物基因組中DNA甲基化能（）

A:增強或者抑制基因活性，視具體基因而定B:增強基因活性C:抑制基因活性D:對基因活性沒有影響

答案:抑制基因活性在堿性條件下，DNA和RNA分別會發(fā)生的是（）

A:分解，分解B:變性，變性C:變性，分解D:分解，變性

答案:變性，分解Tmisafunctionofthe（

）contentofDNAsample,andrangesfrom80℃to100℃fordifferentDNAmolecules.

A:G+CB:A+GC:A+CD:G+T

答案:G+C

第二章單元測試

Asawhole,theE.colichromosomeispositivelysupercoiled.（）

A:錯B:對

答案:錯Ineukaryoticchromosomes,morethan____ofthemassofchromatinisprotein.（）

A:60%B:40%C:30%D:50%

答案:50%Thenucleosomesarewoundintoleft-handedhelix,formasolenoid,witharound____nucleosomesperturn.（）

A:6B:4C:3D:5

答案:65`-CG-3`sequenceinmammalianDNAarenormallymethylatedonthe____.（）

A:CB:GC:CorGD:CandG

答案:CDispersedrepetitiveDNAisrefertomost____DNA.（）

A:uniqueB:moderatelyrepetitiveC:NoneoftheaboveD:highlyrepetitive

答案:moderatelyrepetitive

第三章單元測試

TheE.colioriCcontains____9bpbindingsitesfortheinitiatorproteinDnaA.（）

A:4B:6C:3D:5

答案:4IntheE.coli,bothleadingandlaggingstrandprimersareelongatedby_____holoenzyme.（）

A:DNApolymeraseⅠB:DNApolymeraseⅢC:DNApolymeraseⅡD:DNApolymeraseⅣ

答案:DNApolymeraseⅢ判斷對錯：Definedreplicationoriginsequenceshavenotbeenisolatedfrommammaliancells.（）

A:對B:錯

答案:對TelomeraseisareversetranscriptasethatcarriesitsownRNAmolecule(about（

）nt).

A:200B:150C:100D:50

答案:150Theunlimitedproliferativecapacityofmanycancercellsisassociatedwith（

）oftelomeraseactivity.

A:inactivationB:reactivationC:NoneoftheaboveD:reactivationorinactivation

答案:reactivation

第四章單元測試

What'stheChinesefornonsensemutations?（）

A:沉默突變B:無義突變C:都不是D:錯義突變

答案:無義突變ThemostimportantformcausingDNAdamageis（

）whichproducespyrimidinedimersfromadjacentpyrimidinebases.

A:γ-raysB:VisiblelightC:X-raysD:UVlight

答案:UVlight______oftheDNAafterreplicationallowsthedaughterstrandtobedistinguishedfromtheparentalstrand.（）

A:AcetylationB:HemimethylationC:PhosphorylationD:Methylation

答案:HemimethylationHomologousrecombinationisalsoimportantforDNArepair.Thismechanismhasalsobeencalled_____.（）

A:replicationrepairB:post-replicationrepairC:pre-replicationrepairD:Noneoftheabove

答案:post-replicationrepair判斷：Thegeneintowhichthetransposoninsertsisusuallyactivated,andgenesbetweentwocopiesofatransposoncanbedeletedbyrecombinationbetweenthem.（）

A:對B:錯

答案:錯

第五章單元測試

Inthetranscription,（

）mayalsobecalledthetemplatestrand.

A:antisensestrandB:singlestrandC:doublestrandD:sensestrand

答案:antisensestrand判斷：Inprokaryotes,transcriptionandtranslationaredynamicallycoupled,asthelatterstartsbeforetheformeriscomplete.（）

A:錯B:對

答案:對Followingsuccessfultranscriptioninitiation,the（

）subunitofE.coliRNApolymeraseisreleasedtoformaternarycomplex(threecomponents)ofpolymerase-DNA-nascentRNA,whichisresponsibleforRNAchainelongation.

A:σB:β’C:βD:α

答案:σ判斷：NoprimerisneededforRNAsynthesis.（）

A:錯B:對

答案:對Somegenescontainterminatorsequenceswhichrequireanadditionalproteinfactor,（

）,forefficienttranscriptiontermination.

A:αB:βC:σD:ρ

答案:ρ

第六章單元測試

Thelactose(lac)operonconsistsof（

）structuralgenes.

A:oneB:twoC:fourD:three

答案:threeThelacrepressorismadeupof（

）identicalproteinsubunits..

A:twoB:threeC:oneD:four

答案:fourThetrprepressorisadimerwhichinteractswiththetrpoperatoronlywhenitiscomplexedwith________.（）

A:alanineB:tryptophanC:leucineD:lysine

答案:tryptophanThetrpoperonencodes_____structuralgenesinvolvedintryptophanbiosynthesis.（）

A:fourB:fiveC:threeD:six

答案:fiveTheleadersequenceofthetrpoperonRNAcontainsfourregionsofcomplementarysequencewhichcanformdifferentbase-pairedRNAstructures.Thesearetermedsequences1,2,3and4.Theattenuatorhairpinistheproductofthebasepairingof______.（）

A:sequences1and4B:sequences1and2C:sequences3and4D:sequences2and3

答案:sequences3and4

第七章單元測試

Ineukaryoticcell,（）islocatedinthenucleoplasm.Itisresponsibleforthesynthesisoftheprecursorsof5SrRNA,tRNAsandothersmallnuclearandcytosolicRNAs.

A:RNApolymeraseIB:RNApolymeraseIIIC:RNApolymeraseⅣD:RNApolymeraseII

答案:RNApolymeraseIIIIneukaryoticcell,thelargestsubunitofRNApolymeraseIIhasa_____aminoacidrepeatattheCterminuscalledthecarboxy-terminaldomain(CTD).（）

A:sevenB:fiveC:fourD:six

答案:sevenRNAPolⅢtranscribes5SrRNAcomponentofthesmallribosomalsubunit,whichistheonlyrRNAsubunittobetranscribedseparately.

A:錯B:對

答案:錯SomeRNAPolIIpromotershaveneitheraTATAboxnoraninitiatorelement.Thesegenesaregenerallytranscribedathighrates,andinitiationoftranscriptionmayoccuratdifferentstartsitesoveralengthofupto200bp.

A:錯B:對

答案:錯（）bindstoTATAbox.ItisamultiproteincomplexofTBPandTAFⅡs(TBP-associatedfactors).

A:TFⅡAB:TFⅡCC:TFⅡBD:TFⅡD

答案:TFⅡD

第八章單元測試

（

）isNOTaDNA-bindingdomainofeukaryotictranscriptionfactor。

A:Helix-turn-helixdomainB:Glutamine-richdomainC:BasicdomainD:Zincfingerdomain

答案:Glutamine-richdomainWhichoneofthefollowingisaDNA-bindingdomainofeukaryotictranscriptionfactor?（）

A:Glutamine-richdomainB:ZincfingerdomainC:LeucinezipperD:Proline-richdomain

答案:ZincfingerdomainWhichoneofthefollowingisadimerizationdomainofeukaryotictranscriptionfactor?（）

A:Proline-richdomainB:LeucinezipperC:Helix-turn-helixdomainD:Zincfingerdomain

答案:LeucinezipperWhichoneofthefollowingisatranscriptionactivationdomainofeukaryotictranscriptionfactor?（）

A:Glutamine-richdomainB:BasicdomainC:ZincfingerdomainD:Helix-turn-helixdomain

答案:Glutamine-richdomainSP1contains（

）zincfingermotifsandtwoglutamine-richtransactivationdomains.

A:fiveB:fourC:sixD:three

答案:three

第九章單元測試

Inthenucleolusofeukaryotes,RNApolymeraseI(RNAPolI)transcribestherRNAgenes,whichusuallyexistintandemrepeats,toyieldalong,singlepre-rRNAwhichcontainsonecopyeachofthe18S,5.8Sand28Ssequences

A:錯B:對

答案:對許多真核生物前tRNA是由內(nèi)含子以及額外的5'-和3'-核苷酸合成的，所有這些都在加工過程中被去除。（）

A:對B:錯

答案:對SeveralbiochemicalreactionscanbecarriedoutbyRNAenzymes(ribozymes).（）

A:錯B:對

答案:對ProcessingofeukaryotichnRNAinvolvesseveralevents（）

A:5’-cappingB:3’-cleavageandpolyadenylationC:methylationD:splicing

答案:5’-capping；3’-cleavageandpolyadenylation；methylation；splicingThissplicingreactionoccursinthenucleusandrequirestheintrontohave:（）

A:amethylationsiteB:anAG-3’C:abranchpointsequenceD:a5’-GU

答案:anAG-3’；abranchpointsequence；a5’-GU

第十章單元測試

（

）isthesimpletransferofaclonedfragmentofDNAfromonevectortoanother.

A:CloningB:SubcloningC:PrecloningD:Noneoftheabove

答案:SubcloningSinceplasmidsare（

）E.colichromosomalDNA,theycanbeseparatedfromthelatterbyphysico-chemicalmethods,suchasalkalinelysis.

A:largerthanB:smallerorlargerthanC:smallerthanD:equalto

答案:smallerthan（

）restrictionenzymescutDNAattherecognitionsiteandforthisreasonaremostoftenusedinscientificexperimentation.

A:Type2B:Type4C:Type1D:Type3

答案:Type2Treatmentofthelinearvectormoleculewith（

）willremovethe5’-phosphatesandrenderthevectorunabletoligateintoacirclewithoutaninsertedtarget,soreducingtheproportionofrecreatedvectorinthemixture.

A:restrictionenzymeB:T4DNAligaseC:DNApolymeraseD:alkalinephosphatase

答案:alkalinephosphataseTheE.colicellscanbemadecompetenttotakeupplasmidDNAbytreatmentwith（）

A:Na+B:K+C:Cu2+D:Ca2+

答案:Ca2+

第十一章單元測試

5’-Endlabelingisperformedusing（）toaddaradioactivephosphatetonucleicacidswithafree5’-hydroxylgroup,whichcanbecreatedbydephosphorylationusingalkalinephosphatase.

A:polynucleotidekinaseB:T4DNAligaseC:restrictionenzymesD:DNApolymerase

答案:polynucleotidekinaseInSouthernblotting,thewashingstepshouldbecarriedoutat（）℃belowTmoftheprobeandtargetsequences.

A:12B:5C:6D:10

答案:12Sangerchain-terminationmethodofDNAsequencinguses（）toterminateenzymicallysynthesizedcopiesofatemplate.

A:dNTPsB:NoneoftheaboveC:ddNTPsD:NTPs

答案:ddNTPsThes