各種帶標(biāo)簽的質(zhì)粒

1、產(chǎn)品簡介：產(chǎn)品編號產(chǎn)品名稱產(chǎn)品包裝產(chǎn)品價格D2632pCMV-C-Flag1g798.00元pCMV-C-Flag是碧云天自行研發(fā)的用于在哺乳動物細(xì)胞中表達(dá)C端和Flag tag(Flag標(biāo)簽)融合的目的蛋白的表達(dá)質(zhì)粒。含有CMV啟動子可以高效啟動目的蛋白在細(xì)胞中的表達(dá)；在多克隆位點的3'端含有一個可以編碼Flag標(biāo)簽的序列，因此可以表達(dá)出含有Flag標(biāo)簽的融合蛋白，可以方便地使用抗Flag的抗體來識別目的蛋白，有利于目的蛋白檢測和分離純化。質(zhì)粒為卡那霉素抗性。轉(zhuǎn)染細(xì)胞后，可使用G418篩選穩(wěn)定表達(dá)目的蛋白的細(xì)胞株。    pCMV-C

2、-Flag質(zhì)粒的主要信息如下： Feature Nucleotide                                            &#

3、160;Position    CMV promoter                                           

4、;        1602    T3 promoter and T3 primer binding site                         620639    multiple c

5、loning site                                          680-740    c-Flag tag

6、                                                  

7、  741-764    T7 promoter and T7 primer binding site                         817838    SV40 polyA signal     &#

8、160;                                        8501233    f1 origin of ss-DNA repli

9、cation                                13711677    bla promoter           &#

10、160;                                       17021826    SV40 promoter   

11、0;                                              18462184  &#

12、160; neomycin/kanamycin resistance ORF                              22193010    HSV-thymidine kinase (TK) polyA signal   

13、;                      30113469    pUC origin                     

14、0;                               35984265    pCMV-C-Flag質(zhì)粒的圖譜如下：         pCMV-C-

15、Flag的多克隆位點的詳細(xì)圖譜如下：                                       XmaI        &

16、#160;        PstI         SacI                          Small   BamHI  Hind

17、III    651 GAGCTCCACC GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCAAGCTTCT        CTCGAGGTGG CGCCACCGCC GGCGAGATCG GGCCCGCCTA GGTTCGAAGA                   

18、;                                       Flag           

19、 EcoRI  EcoRV SacI   BglII XhoI   XbaI   D  Y  K  D    701 GCAGGAATTC GATATCGTCG ACAGATCTCT CGAGTCTAGA GATTACAAGG        CGTCCTTAAG CTATAGCAGC TGTCTAGAGA GCTCAGATCT CTAATGTTCC 

20、0;      tag                     D  D  D   K     ApaI    751 ATGACGACGA TAAGTAAGGG CCCGGTACCT TAATTAATTA A

21、GGTACCAGG        TACTGCTGCT ATTCATTCCC GGGCCATGGA ATTAATTAAT TCCATGGTCC     pCMV-C-Flag中沒有的酶切位點(Restriction enzymes that do not cut pCMV-C-Flag)包括：    Afl II    Age I    Ahd I   &#

22、160;   Asc I     Bbs I    Bbv II      Blp I     Bsg I     BsiW I   BsmB I      BspM II   BsrG I   BssH II     

23、Bst1107 I    BstE II   Ear I    Eco47 III   Eco72 I   EcoN I   Esp I       Fse I    Nru I     PflM I   Pme I       

24、Pml I     PpuM I   Psp1406 I   Sap I    Sca I     Spe I    pCMV-C-Flag中的單酶切位點(Restriction enzymes that cut pCMV-C-Flag once)包括：Nde I      CATA,TG      

25、;  241SnaB I     TAC|GTA         347Nhe I      GCTAG,C        598Sac I      G,AGCTC        656Sac II  

26、60;  CC,GCGG        663BstX I     CCAN,NNNNNTGG  664Not I      GCGGCC,GC      669PspA I     CCCGG,G        681Xma I  

27、60;   CCCGG,G        681Srf I      GCCC|GGGC       683Sma I      CCC|GGG         683BamH I     GGATC,C  &

28、#160;     688Hind III   AAGCT,T        694Pst I      C,TGCAG        704EcoR I     GAATT,C        706EcoR V  &#

29、160;  GAT|ATC         714Sal I      GTCGA,C        718Acc I      GTMK,AC        719Bgl II     AGATC,T  &#

30、160;     724PaeR7 I    CTCGA,G        730Xho I      CTCGA,G        730Xba I      TCTAG,A        736Bsp120

31、 I   GGGCC,C        769Apa I      G,GGCCC        773Pvu I       CG,ATCG           851Bcl I     

32、  TGATC,A           1005Mun I       CAATT,G           1098Hpa I       GTT|AAC         

33、0;  1111Mlu I       ACGCG,T           1234Dra III     CAC,NNNGTG        1464Sfi I       GGCCN,NNNNGGCC    2123

34、BseR I      GAGGAG 16/14       2166Stu I       AGG|CCT            2169Cla I       ATCG,AT       

35、0;   2188Kas I       GGCGC,C           2347Nar I       GGCG,CC           2348Ehe I       GGC|GC

36、C            2349Bbe I       G,GCGCC           2351Msc I       TGG|CCA           

37、0;2430Tth111 I    GACNN,NGTC        2466BsrD I      GCAATG, 8          2581Bsp1286 I   G,DGCHC           2651Rsr II 

38、0;    CGGWC,CG          2864BsiC I      TTCG,AA           3030BstB I      TTCG,AA          

39、; 3030Bsa I       GGTCTC 7/11        3337HgiE II     ACCNNNNNNGGT-1/13  3677ApaL I      GTGCA,C           3952    &

40、#160;pCMV-C-Flag質(zhì)粒中推薦使用的測序引物序列如下：    T3 primer(620639): 5AATTAACCCTCACTAAAGGG 3    T7 primer(817-838): 5GTAATACGACTCACTATAGGGC 3    pCMV-C-Flag的全序列信息： D2632 pCMV-C-Flag-seq.txt產(chǎn)品簡介：產(chǎn)品編號產(chǎn)品名稱產(chǎn)品包裝產(chǎn)品價格D2672pCMV-C-Myc1g798.00元   &

41、#160;pCMV-C-Myc是碧云天自行研發(fā)的用于在哺乳動物細(xì)胞中表達(dá)C端和Myc tag(Myc標(biāo)簽)融合的目的蛋白的表達(dá)質(zhì)粒。含有CMV啟動子可以高效啟動目的蛋白在細(xì)胞中的表達(dá)；在多克隆位點的3'端含有一個可以編碼Myc標(biāo)簽的序列，因此可以表達(dá)出含有Myc標(biāo)簽的融合蛋白，可以方便地使用抗Myc的抗體來識別目的蛋白，有利于目的蛋白檢測和分離純化。質(zhì)粒為卡那霉素抗性。轉(zhuǎn)染細(xì)胞后，可使用G418篩選穩(wěn)定表達(dá)目的蛋白的細(xì)胞株。    pCMV-C-Myc質(zhì)粒的主要信息如下：    Feature N

42、ucleotide                                             Position   

43、 CMV promoter                                                

44、;   1602    T3 promoter and T3 primer binding site                         620639    multiple cloning site    

45、                                      680-740    c-Myc tag      &

46、#160;                                               741-770 

47、   T7 promoter and T7 primer binding site                         823844    SV40 polyA signal         

48、60;                                    8561239    f1 origin of ss-DNA replication    

49、                            13771683    bla promoter               

50、60;                                   17081832    SV40 promoter        

51、;                                          18522190    neomycin/kanamy

52、cin resistance ORF                              22253016    HSV-thymidine kinase(TK)polyA signal       &#

53、160;                   30173475    pUC origin                        &

54、#160;                            36044271    pCMV-C-Myc質(zhì)粒的圖譜如下：           pCMV-C-Myc的

55、多克隆位點的詳細(xì)圖譜如下：                                       XmaI         

56、        PstI        SacI                           SmaI    BamHI  Hind

57、III    651 GAGCTCCACC GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCAAGCTTCT        CTCGAGGTGG CGCCACCGCC GGCGAGATCG GGCCCGCCTA GGTTCGAAGA                   

58、;                                        Myc         &#

59、160;  EcoRI  EcoRV SalI   BglII XhoI   XbaI   E  Q  K  L    701 GCAGGAATTC GATATCGTCG ACAGATCTCT CGAGTCTAGA GAGCAGAAAC        CGTCCTTAAG CTATAGCAGC TGTCTAGAGA GCTCAGATCT CTCGTCTTTG

60、        tag                            I  S  E   E  D  L      ApaI

61、60;   751 TCATCTCTGA AGAGGATCTG TAAGGGCCCG GTACCTTAAT TAATTAAGGT        AGTAGAGACT TCTCCTAGAC ATTCCCGGGC CATGGAATTA ATTAATTCCA    pCMV-C-Myc中沒有的酶切位點(Restriction enzymes that do not cut pCMV-C-Myc)包括：    Af

62、l II    Age I       Ahd I     Asc I     Bbs I       Bbv II    Blp I    Bsg I     BsiW I      BsmB I  

63、;  BspM II   BsrG I      BssH II   Bst1107 I    BstE II   Eco47 III   Eco72 I   EcoN I    Esp I       Fse I     Nru I  &

64、#160; PflM I    Pme I       Pml I     PpuM I    Psp1406 I   Sap I     Sca I    Spe I     Spl I    pCMV-C-Myc中的單酶切位點(Restricti

65、on enzymes that cut pCMV-C-Myc once)包括：Nde I      CATA,TG       241SnaB I     TAC|GTA        347Nhe I      GCTAG,C       598Sac I  

66、;    G,AGCTC       656Sac II     CC,GCGG       663BstX I     CCAN,NNNNNTGG 664Not I      GCGGCC,GC     669PspA I    

67、0;CCCGG,G       681Xma I      CCCGG,G       681Srf I      GCCC|GGGC      683Sma I      CCC|GGG        683

68、BamH I     GGATC,C       688Hind III   AAGCT,T       694Pst I      C,TGCAG       704EcoR I     GAATT,C      &#

69、160;706EcoR V     GAT|ATC        714Sal I      GTCGA,C       718Acc I      GTMK,AC       719Bgl II     AGATC,T 

70、0;     724PaeR7 I    CTCGA,G       730Xho I      CTCGA,G       730Xba I      TCTAG,A       736Ear I    &#

71、160; CTCTTC 7/10    755Bsp120 I   GGGCC,C       775Apa I      G,GGCCC       779Pvu I       CG,ATCG          &#

72、160;857Bcl I       TGATC,A          1011Mun I       CAATT,G          1104Hpa I       GTT|AAC     

73、60;     1117Mlu I       ACGCG,T          1240Dra III     CAC,NNNGTG       1470Sfi I       GGCCN,NNNNGGCC   212

74、9BseR I      GAGGAG 16/14      2172Stu I       AGG|CCT           2175Cla I       ATCG,AT         

75、60;2194Kas I       GGCGC,C          2353Nar I       GGCG,CC          2354Ehe I       GGC|GCC      &#

76、160;    2355Bbe I       G,GCGCC          2357Msc I       TGG|CCA           2436Tth111 I    GACNN,NGTC  &#

77、160;    2472BsrD I      GCAATG, 8         2587Bsp1286 I   G,DGCHC          2657Rsr II      CGGWC,CG       

78、;  2870BsiC I      TTCG,AA          3036BstB I      TTCG,AA          3036Bsa I       GGTCTC 7/11     

79、;  3343HgiE II     ACCNNNNNNGGT-1/13 3683ApaL I      GTGCA,C          3958     pCMV-C-Myc質(zhì)粒中推薦使用的測序引物序列如下：    T3 primer(620639): 5AATTAACCCTCACTAAAGGG 3 &#

80、160;  T7 primer(823-844): 5GTAATACGACTCACTATAGGGC 3    pCMV-C-Myc的全序列信息： D2672 pCMV-C-Myc-seq.txt產(chǎn)品編號產(chǎn)品名稱產(chǎn)品包裝產(chǎn)品價格D2722pCMV-N-Flag1g798.00元    pCMV-N-Flag是碧云天自行研發(fā)的用于在哺乳動物細(xì)胞中表達(dá)N端和Flag tag(Flag標(biāo)簽)融合的目的蛋白的表達(dá)質(zhì)粒。含有CMV啟動子可以高效啟動目的蛋白在細(xì)胞中的表達(dá)；在多克隆位點的5'

81、端含有一個可以編碼Flag標(biāo)簽的序列，因此可以表達(dá)出含有Flag標(biāo)簽的融合蛋白，可以方便地使用抗Flag的抗體來識別目的蛋白，有利于目的蛋白檢測和分離純化。質(zhì)粒為卡那霉素抗性。轉(zhuǎn)染細(xì)胞后，可使用G418篩選穩(wěn)定表達(dá)目的蛋白的細(xì)胞株。    pCMV-N-Flag質(zhì)粒的主要信息如下：    Feature Nucleotide                 

82、                Position    CMV promoter                           

83、60;           1602    T3 promoter and T3 primer binding site             620639    Flag tag          

84、;                                 679702    multiple cloning site         &

85、#160;                    705778    T7 promoter and T7 primer binding site             821842    SV40

86、 polyA signal                                  8541237    f1 origin of ss-DNA replication     &

87、#160;              13751681    bla promoter                            

88、0;          17061830    SV40 promoter                                 

89、     18502188    neomycin/kanamycin resistance ORF                  22233014    HSV-thymidine kinase(TK)polyA signal      

90、60;        30153473    pUC origin                                   &#

91、160;     36024269    pCMV-N-Flag質(zhì)粒的圖譜如下：           pCMV-N-Flag的多克隆位點的詳細(xì)圖譜如下：                    

92、0;                         Flag tag                SacI       

93、60;               M  D   Y  K  D   D  D  D  K    651 GAGCTCCACC GCGGTGGCGG CCGCCATGGA TTACAAGGAT GACGACGATA        

94、CTCGAGGTGG CGCCACCGCC GGCGGTACCT AATGTTCCTA CTGCTGCTAT            XmaI                 PstI            &#

95、160;SmaI    BamHI HindIII      EcoRI  EcoRV SalI   BglII    701 AGAGCCCGGG CGGATCCAAG CTTCTGCAGG AATTCGATAT CGTCGACAGA        TCTCGGGCCC GCCTAGGTTC GAAGACGTCC TTAAGCTATA GCAGCTGTCT 

96、;          XhoI  XbaI          ApaI    751 TCTCTCGAGT CTAGAACTAG TGGGCCCGGT ACCTTAATTA ATTAAGGTAC        AGAGAGCTCA GATCTTGATC ACCCGGGCCA TG

97、GAATTAAT TAATTCCATG    pCMV-N-Flag中沒有的酶切位點(Restriction enzymes that do not cut pCMV-N-Flag)包括：    Afl II    Age I    Ahd I       Asc I     Bbs I    Bbv II  

98、0;   Blp I    Bsg I     BsiW I   BsmB I      BspM II   BsrG I   BssH II     Bst1107 I    BstE II   Ear I    Eco47 III   

99、Eco72 I   EcoN I   Esp I       Fse I    Nru I     PflM I   Pme I       Pml I     PpuM I   Psp1406 I   Sap I    

100、;Sca I     Spl I     pCMV-N-Flag中的單酶切位點(Restriction enzymes that cut pCMV-N-Flag once)包括：Nde I      CATA,TG       241SnaB I     TAC|GTA        347Nhe

101、I      GCTAG,C       598Sac I      G,AGCTC       656Sac II     CC,GCGG       663BstX I     CCAN,NNNNNTGG 664Not I  

102、    GCGGCC,GC     669PspA I     CCCGG,G       706Xma I      CCCGG,G       706Srf I      GCCC|GGGC      70

103、8Sma I      CCC|GGG        708BamH I     GGATC,C       713Hind III   AAGCT,T       719Pst I      C,TGCAG    &#

104、160;  729EcoR I     GAATT,C       731EcoR V     GAT|ATC        739Sal I      GTCGA,C       743Acc I      

105、GTMK,AC       744Bgl II     AGATC,T       749PaeR7 I    CTCGA,G       755Xho I      CTCGA,G       755Xba I   &

106、#160;  TCTAG,A       761Spe I      ACTAG,T       767Bsp120 I   GGGCC,C       773Apa I      G,GGCCC       777Pv

107、u I       CG,ATCG           855Bcl I       TGATC,A          1009Mun I       CAATT,G      &

108、#160;   1102Hpa I       GTT|AAC           1115Mlu I       ACGCG,T          1238Dra III     CAC,NNNGTG  &#

109、160;    1468Sfi I       GGCCN,NNNNGGCC   2127BseR I      GAGGAG 16/14      2170Stu I       AGG|CCT           2

110、173Cla I       ATCG,AT          2192Kas I       GGCGC,C          2351Nar I       GGCG,CC      &

111、#160;   2352Ehe I       GGC|GCC           2353Bbe I       G,GCGCC          2355Msc I       TGG|CCA &

112、#160;         2434Tth111 I    GACNN,NGTC       2470BsrD I      GCAATG, 8         2585Bsp1286 I   G,DGCHC      

113、    2655Rsr II      CGGWC,CG         2868BsiC I      TTCG,AA          3034BstB I      TTCG,AA     &#

114、160;    3034Bsa I       GGTCTC 7/11       3341HgiE II     ACCNNNNNNGGT-1/13 3681ApaL I      GTGCA,C          3956 pCMV-N-Flag質(zhì)粒中推薦

115、使用的測序引物序列如下：    T3 primer(620639): 5AATTAACCCTCACTAAAGGG 3    T7 primer(821-842): 5GTAATACGACTCACTATAGGGC 3    pCMV-N-Flag的全序列信息： D2722 pCMV-N-Flag-seq.txt產(chǎn)品編號產(chǎn)品名稱產(chǎn)品包裝產(chǎn)品價格D2639pCMV-C-HA1g798.00元    pCMV-C-HA是碧云天自行研發(fā)的用于在哺

116、乳動物細(xì)胞中表達(dá)C端和HA tag(HA標(biāo)簽)融合的目的蛋白的表達(dá)質(zhì)粒。含有CMV啟動子可以高效啟動目的蛋白在細(xì)胞中的表達(dá)；在多克隆位點的3'端含有一個可以編碼HA標(biāo)簽的序列，因此可以表達(dá)出含有HA標(biāo)簽的融合蛋白，可以方便地使用抗HA的抗體來識別目的蛋白，有利于目的蛋白檢測和分離純化。質(zhì)粒為卡那霉素抗性。轉(zhuǎn)染細(xì)胞后，可使用G418篩選穩(wěn)定表達(dá)目的蛋白的細(xì)胞株。    pCMV-C-HA質(zhì)粒的主要信息如下：    Feature Nucleotide     

117、                                        Position    CMV promoter   

118、60;                                               1602  

119、;  T3 promoter and T3 primer binding site                         620639    multiple cloning site         

120、0;                                680-740    c-HA tag            

121、                                           741-767    T7 promoter

122、 and T7 primer binding site                         820841    SV40 polyA signal              &#

123、160;                               8531236    f1 origin of ss-DNA replication         

124、;                       13741680    bla promoter                    &#

125、160;                              17051829    SV40 promoter            

126、0;                                     18492187    neomycin/kanamycin resistance ORF  

127、0;                           22223013    HSV-thymidine kinase (TK) polyA signal            

128、;             30143472    pUC origin                              

129、0;                      36014268    pCMV-C-HA質(zhì)粒的圖譜如下：        pCMV-C-HA的多克隆位點的詳細(xì)圖譜如下：       

130、0;                               XmaI                 PstI &

131、#160;      SacI                           Small   BamHI  HindIII    651 GAGCTCCACC GCGGTGGCGG CCGCTCTAGC

132、CCGGGCGGAT CCAAGCTTCT        CTCGAGGTGG CGCCACCGCC GGCGAGATCG GGCCCGCCTA GGTTCGAAGA                             

133、60;                              HA            EcoRI  EcoRV SacI   BglII Xh

134、oI   XbaI   Y  P  Y  D    701 GCAGGAATTC GATATCGTCG ACAGATCTCT CGAGTCTAGA TACCCATACG        CGTCCTTAAG CTATAGCAGC TGTCTAGAGA GCTCAGATCT ATGGGTATGC        tag &#

135、160;                      V  P  D   Y  A      ApaII    751 ATGTTCCAGA TTACGCTTAA GGGCCCGGTA CCTTAATTAA TTAAGGTACC &

136、#160;      TACAAGGTCT AATGCGAATT CCCGGGCCAT GGAATTAATT AATTCCATGG    pCMV-C-HA中沒有的酶切位點(Restriction enzymes that do not cut pCMV-C-HA)包括：    Age I    Ahd I       Asc I    

137、 Bbs I    Bbv II      Blp I       Bsg I    BsiW I   BsmB I      BspM II   BsrG I   BssH II     Bst1107 I   BstE II 

138、   Ear I    Eco47 III   Eco72 I   EcoN I   Esp I       Fse I       Nru I    PflM I   Pme I       Pml I    

139、; PpuM I   Psp1406 I   Sap I       Sca I    Spe I    Spl I    pCMV-C-HA中的單酶切位點(Restriction enzymes that cut pCMV-C-HA once)包括：Nde I      CATA,TG    &#

140、160;   241SnaB I     TAC|GTA         347Nhe I      GCTAG,C        598Sac I      G,AGCTC        656Sac II

141、    CC,GCGG        663BstX I     CCAN,NNNNNTGG  664Not I      GCGGCC,GC      669PspA I     CCCGG,G        681Xma I

142、     CCCGG,G        681Srf I      GCCC|GGGC       683Sma I      CCC|GGG         683BamH I     GGATC,

143、C        688Hind III   AAGCT,T        694Pst I      C,TGCAG        704EcoR I     GAATT,C        706EcoR V

144、     GAT|ATC         714Sal I      GTCGA,C        718Acc I      GTMK,AC        719Bgl II     AGATC,T

145、        724PaeR7 I    CTCGA,G        730Xho I      CTCGA,G        730Xba I      TCTAG,A        

146、736Afl II     CTTAA,G        767Bsp120 I   GGGCC,C        772Apa I      G,GGCCC        776Pvu I      CG,ATCG  

147、         854Bcl I      TGATC,A          1008Mun I      CAATT,G          1101Hpa I      GTT

148、|AAC           1114Mlu I      ACGCG,T          1237Dra III    CAC,NNNGTG       1467Sfi I      GGCCN,NNNNGGCC &

149、#160; 2126BseR I     GAGGAG 16/14      2169Stu I      AGG|CCT           2172Cla I      ATCG,AT          2

150、191Kas I      GGCGC,C          2350Nar I      GGCG,CC          2351Ehe I      GGC|GCC         &

151、#160; 2352Bbe I      G,GCGCC          2354Msc I      TGG|CCA           2433Tth111 I   GACNN,NGTC       2469Bsr

152、D I     GCAATG, 8         2584Bsp1286 I  G,DGCHC          2654Rsr II     CGGWC,CG         2867BsiC I     TTCG,

153、AA          3033BstB I     TTCG,AA          3033Bsa I      GGTCTC 7/11       3340HgiE II    ACCNNNNNNGGT-1/13 3680ApaL I

154、     GTGCA,C          3955     pCMV-C-HA質(zhì)粒中推薦使用的測序引物序列如下：    T3 primer(620639): 5AATTAACCCTCACTAAAGGG 3    T7 primer(820-841): 5GTAATACGACTCACTATAGGGC 3    

155、;pCMV-C-HA的全序列信息： D2639 pCMV-C-HA-seq.txt產(chǎn)品編號產(chǎn)品名稱產(chǎn)品包裝產(chǎn)品價格D2650pCMV-C-His1g798.00元    pCMV-C-His是碧云天自行研發(fā)的用于在哺乳動物細(xì)胞中表達(dá)C端和His tag(His標(biāo)簽)融合的目的蛋白的表達(dá)質(zhì)粒。含有CMV啟動子可以高效啟動目的蛋白在細(xì)胞中的表達(dá)；在多克隆位點的5'端含有一個可以編碼His標(biāo)簽的序列，因此可以表達(dá)出含有His標(biāo)簽的融合蛋白，可以方便地使用抗His的抗體來識別目的蛋白，有利于目的蛋白檢測和分離純化。質(zhì)粒為卡那霉素抗性。轉(zhuǎn)染細(xì)胞后

156、，可使用G418篩選穩(wěn)定表達(dá)目的蛋白的細(xì)胞株。    pCMV-C-His質(zhì)粒的主要信息如下：    Feature Nucleotide                                

157、0;            Position    CMV promoter                               &

158、#160;                   1602    T3 promoter and T3 primer binding site                    

159、     620639    multiple cloning site                                     &#

160、160;    680-740    c-His tag                                       

161、60;              741-758    T7 promoter and T7 primer binding site                         811832    SV40 polyA signal