各種緩沖液的配制方法大全

1、磷酸氫二鈉檸檬酸緩沖液PH0.2mol/L Na2HPO4（毫升）0.1mol/L檸檬酸（毫升）pH0.2mol/L Na2HPO4（毫升）0.1mol/L檸檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.87.07.27.

2、47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量 = 14.98，0.2mol/L溶液為28.40克/升。Na2HPO4·2H2O分子量 = 178.05，0.2mol/L溶液含35.01克/升。C4H2O7·H2O分子量 = 210.14，0.1mol/L溶液為21.01克/升20%鹽酸溶液如何配置：取濃鹽酸（質(zhì)量

3、百分比濃度為36.5%）一個體積（如100毫升），加入到96.5毫升水中就可以了。36.5%*100*1.17=20%*mm=213.5(克所需水的質(zhì)量為;213.5-117=96.5克，也就是96.5毫升水。磷酸鹽緩沖液配制方法（PB）磷酸鹽緩沖液（Phosphate Buffer, PB）試劑： NaH2PO4·2H2O     Na2HPO4·12H2O配制方法：配制時，常先配制0.2mol/L的 NaH2PO4和0.2mol/L的Na2HPO4，兩者按一定比例混合即成0.2mol/L的磷酸鹽緩沖液（PB），根據(jù)需要可配制不同濃度的PB和PBS。（

4、1）0.2mol/L的 NaH2PO4；稱取NaH2PO4.2H2O 31.2g(或 NaH2PO4·H2O 27.6g加重蒸水至1000ml溶解。（2）0.2mol/L的 Na2HPO4：稱取Na2HPO4.12H2O 71.632g（或 Na2HPO4·7H2O 53.6g或 Na2HPO4·2H2O 35.6g）加重蒸水至1000ml溶解。（3）0.2mol/L pH7.4的PB的配制：取19ml 0.2mol/L的 NaH2PO4和81ml 0.2mol/L的 Na2HPO4·12H2O,充分混合即為0.2mol/L的PB（pH約為7.47.5）

5、。若pH偏高或偏低，可通過改變二者的比例來加以調(diào)整，室溫保存即可。0.2mol/L磷酸鹽緩沖液（pH5.78.0）pH   0.2mol/L NaH2PO4(ml   0.2mol/L Na2HPO4(ml5.7           93.5                       6.55.8           92.0      

6、;                 8.05.9           90.0                     10.06.0           87.7                     12.36.1

7、           85.0                     15.0 6.2           81.5                     18.56.3           77.5       &

8、#160;             22.56.4           73.5                     26.56.5           68.5                     31.56.6   &

9、#160;       62.5                     37.56.7           56.5                     43.56.8           51.0         

10、0;           49.06.9           45.0                     55.07.0           39.0                     61.07.1     

11、0;     33.0                     67.07.2           28.0                     72.07.3           23.0            

12、        67.07.4           19.0                     81.07.5           16.0                     84.07.6        

13、  13.0                     87.07.7           10.5                     89.5 7.8             8.5            

14、60;       91.57.9             7.0                     93.08.0             5.3                     94.70.05mol/L磷酸鹽緩沖液(pH7.0如何

15、配制？PH3-10緩沖溶液的配制方法1磷酸氫二鈉檸檬酸緩沖液pH0.2mol/L Na2HPO4（毫升）0.1mol/L檸檬酸（毫升）pH0.2mol/L Na2HPO4（毫升）0.1mol/L檸檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06

16、.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量 = 14.98，0.2 mol/L溶液為28.40克/升。Na2HPO4·2H2O分子量 = 178.05，0.2 mol/L溶液含35.01克/升。C4H2O7·H2O分子量 = 210.14，0.1 mol/L溶液為21.01

17、克/升。2檸檬酸氫氧化鈉-鹽酸緩沖液pH鈉離子濃度（mol/L）檸檬酸（克）C6H8O7·H2O氫氧化鈉(克NaOH 97%鹽酸（毫升）HCl（濃）最終體積（升）2.23.13.34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010     使用時可以每升中加入1克克酚，若最后pH值有變化，再用少量50% 氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。3 甘氨酸鹽酸緩沖液（0.

18、05mol/L）X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀釋至200毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量 = 75.07，0.2 mol/L甘氨酸溶液含15.01克/升。 4鄰苯二甲酸鹽酸緩沖液（0.05 mol/L）X毫升0.2 mol/L鄰苯二甲酸氫鉀 + 0.2 mol/L HCl，再加水稀釋到20毫升pH(20XYpH(20XY2.22.42.62.83.0555554.0703.9603.2952.6422.0

19、223.23.43.63.855551.4700.9900.5970.263鄰苯二甲酸氫鉀分子量 = 204.23，0.2 mol/L鄰苯二甲酸氫溶液含40.85克/升  5檸檬酸檸檬酸鈉緩沖液（0.1 mol/L）pH0.1   mol/L檸檬酸（毫升）0.1   mol/L檸檬酸鈉（毫升）pH0.1   mol/L檸檬酸（毫升）0.1   mol/L檸檬酸鈉（毫升）3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410

20、.39.21.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.018.6檸檬酸C6H8O7·H2O：分子量210.14，0.1 mol/L溶液為21.01克/升。檸檬酸鈉Na3 C6H5O7·2H2O：分子量294.12，0.1 mol/L溶液為29.41克/升。 檸檬酸C6H8O7·H2O: 2.1g檸檬酸鈉Na3 C6H5O7·2H2O: 2.94gH2O: 10

21、0 ml6乙酸乙酸鈉緩沖液（0.2 mol/L） pH(180.2   mol/LNaAc(毫升0.3   mol/LHAc(毫升 pH(180.2   mol/LNaAc(毫升0.3   mol/LHAc(毫升2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac·3

22、H2O分子量 = 136.09，0.2 mol/L溶液為27.22克/升。 7磷酸鹽緩沖液（1）磷酸氫二鈉磷酸二氫鈉緩沖液（0.2） pH0.2   mol/LNa2HPO4(毫升0.3   mol/LNaH2PO4(毫升 pH0.2   mol/L Na2HPO4(毫升0.3   mol/LNaH2PO4(毫升5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.09

23、0.087.785.081.577.573.568.562.556.551.045.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3Na2HPO4·2H2O分子量 = 178.05，0.2 mol/L溶液為85.61克/升。Na2HPO4·12H2O分子量 = 358.22，0.2 mol/L溶液為71.64克/升。Na2HPO4·2H2O分子量 = 156.03，0.2 mo

24、l/L溶液為31.21克/升。（2）磷酸氫二鈉磷酸二氫鉀緩沖液（1/15 mol/L）pHmol/L/15Na2HPO4(毫升mol/L/15KH2PO4(毫升pHmol/L/15Na2HPO4(毫升mol/L/15KH2PO4(毫升4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.678.187.008.009.009.509.759.9010.003.002.001.000.500.250.100Na2HP

25、O4·2H2O分子量 = 178.05，1/15M溶液為11.876克/升。KH2PO4分子量 = 136.09，1/15M溶液為9.078克/升。 8磷酸二氫鉀氫氧化鈉緩沖液（0.05M）X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀釋至29毫升pH(20X (毫升Y（毫升）pH(20X (毫升Y（毫升）5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.3657.07.27.47.67.88.05555552.9633.5003.9504.2804.5204.680 9巴比妥鈉-鹽酸緩沖液（18

26、）pH0.04M巴比妥鈉溶液0.2V鹽酸pH0.04M巴比妥鈉溶液(毫升0.2N鹽酸(毫升6.87.07.27.47.67.88.08.210010010010010010010010018.417.816.715.313.411.479.397.218.48.68.89.09.29.49.61001001001001001001001005.213.822.521.651.130.700.35巴比妥鈉鹽分子量=206.18;0.04M溶液為8.25克/升 10Tris鹽酸緩沖液（0.05M，25）50毫升0.1M三羥甲基氨基甲烷（Tris）溶液與X毫升0.1N鹽酸混勻后，加水稀釋至

27、100毫升。pHX(毫升pHX(毫升7.107.207.307.407.507.607.707.807.908.0045.744.743.442.040.338.536.634.532.029.28.108.208.308.408.508.608.708.808.9026.222.919.917.214.712.410.38.57.0三羥甲基氨基甲烷（Tris）HOCH2 CH2OHCHOCH2 NH2分子量=121.14;0. 1M溶液為12.114克/升。Tris溶液可從空氣中吸收二氧化碳，使用時注意將瓶蓋嚴。 11硼酸硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.47.67.88.01.01.52.03.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂Na2B4O7·H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。 11硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升