常用緩沖溶液地配制

1、標(biāo)準(zhǔn)實(shí)用常用緩沖溶液的配制方法1 .甘氨酸-鹽酸緩沖液(0.05mol/L)X毫升0.2 mol/L 甘氨酸+Y毫升0.2 mol/L HCI，再加水稀釋至 200毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量=75.07，0.2 mol/L 甘氨酸溶液含15.01克/升。2 .鄰苯二甲酸-鹽酸緩沖液(0.05 mol/L )X毫升0.2 mol/L 鄰苯二甲酸氫鉀+ 0.2 mol/L HCl，再加水稀釋到 20毫升pH(20 C)XYpH(20 C)XY2.22.42

2、.62.83.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263鄰苯二甲酸氫鉀分子量=204.23 , 0.2 mol/L 鄰苯二甲酸氫溶液含40.85克/升3 .磷酸氫二鈉-檸檬酸緩沖液pH0.2 mol/L0.1 mol/LpH0.2 mol/L0.1 mol/LNa2HPO4（毫升）檸檬酸（毫升）Na2HPO 4（毫升）檸檬酸（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.8

3、12.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4 分子量 =14.98 , 0.2 mo

4、l/L 溶液為 28.40 克/升。Na2HPO42H2O 分子量 =178.05 , 0.2 mol/L 溶液含 35.01 克/升。C4H2O7H2O 分子量 =210.14 , 0.1 mol/L 溶液為 21.01 克/升。4 檸檬酸-氫氧化鈉-鹽酸緩沖液pH鈉離子濃度（mol/L ）檸檬酸（克）C6H8O7 H2O氫氧化鈉（克）NaOH97%鹽酸（毫升）HCl （濃）最終體積（升）2.23.13.34.35.35.86.50.200.200.200.200.350.450.38210210210210245285266848383831441861561601161064568105

5、12610101010101010 使用時(shí)可以每升中加入1克克酚，若最后pH值有變化，再用少量 50%氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。5 檸檬酸-檸檬酸鈉緩沖液（0.1 mol/L ）pH0.1 mol/L檸檬酸（毫升）0.1 mol/L檸檬酸鈉（毫升）pH0.1 mol/L檸檬酸（毫升）0.1 mol/L檸檬酸鈉（毫升）3.03.23.43.63.84.018.617.216.014.914.013.11.42.84.05.16.06.95.05.25.45.65.86.08.27.36.45.54.73.811.812.713.614.515.316.24.212.37.76.22.8

6、17.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8檸檬酸C6H8O7H2O:分子量210.14 , 0.1 mol/L 溶液為21.01克/升。檸檬酸鈉 Na 3 C6H5O7 2H 2O :分子量294.12 , 0.1 mol/L 溶液為29.41克/毫升。6 .乙酸-乙酸鈉緩沖液(0.2 mol/L )pH（18 C）0.2 mol/LNaAc（毫升）0.3 mol/LHAc（毫升）pH（18 C）0.2 mol/LNaAc（毫升）0.3 mol/LHAc（毫升）2.63.84.04.24.44.60.751.201.802.65

7、3.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac 3H 2O 分子量 =136.09 ，0.2 mol/L 溶液為 27.22 克/ 升。7 磷酸鹽緩沖液(1 )磷酸氫二鈉-磷酸二氫鈉緩沖液(0.2 )pH0.2 mol/LNa2HPO4（毫升）0.3 mol/LNaH 2PO4（毫升）pH0.2 mol/LNa2HPO 4（毫升）0.3 mol/LNaH 2PO4（毫升）5.88.092.07.061.039.05.910.090.07

8、.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO42H2O 分子量 =178.05 , 0.2 mol/L 溶液為 85.61 克/升。Na2HPO4l2H2O 分子量 =358.22 ，0.2 mol/

9、L 溶液為 71.64 克/升。Na2HPO4 2H2O 分子量 =156.03 , 0.2 mol/L 溶液為 31.21 克/升。(2)磷酸氫二鈉-磷酸二氫鉀緩沖液(1/15 mol/L )pHmol/L/15Na 2HPmol/L/15KH 2PpHmol/L/15Na 2Hmol/L/15KH 2PO4(毫升)O4（毫升）PO4（毫升）O4（毫升）4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.678

10、.187.008.009.009.509.759.9010.003.002.001.000.500.250.100Na2HPO4 2H2O 分子量 =178.05 , 1/15M 溶液為 11.876 克/升。KH2PO4 分子量 =136.09 , 1/15M 溶液為 9.078 克/升。8 .磷酸二氫鉀-氫氧化鈉緩沖液（0.05M ）X毫升0.2M K 2PO4 + Y毫升0.2N NaOH 加水稀釋至29毫升X （毫升）Y （毫升）pH(20 C)X （毫升）Y （毫升）pH(20 C)5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.

11、3657.07.27.47.67.88.05555552.9633.5003.9504.2804.5204.6809 .巴比妥鈉-鹽酸緩沖液（18 C）pH0.04M 巴比妥鈉溶液0.2V鹽酸pH0.04M 巴比妥鈉溶液（毫升）0.2N 鹽酸（毫升）6.87.07.27.47.67.88.08.210010010010010010010010018.417.816.715.313.411.479.397.218.48.68.89.09.29.49.61001001001001001001001005.213.822.521.651.130.700.35巴比妥鈉鹽分子量 =206.18;0.04

12、M 溶液為8.25克/升10 . Tris -鹽酸緩沖液（0.05M , 25 C）50毫升0.1M三羥甲基氨基甲烷（Tris）溶液與X毫升0.1N鹽酸混勻后，加水稀釋至100毫升。pHX（毫升）pHX（毫升）7.107.207.307.407.507.607.707.807.908.0045.744.743.442.040.338.536.634.532.029.28.108.208.308.408.508.608.708.808.9026.222.919.917.214.712.410.38.57.0三羥甲基氨基甲烷（Tris）H0CH2CH20HCH0CH2NH2分子量=121.14;0

13、. 1M溶液為12.114克/升。Tris溶液可從空氣中吸收二氧化碳，使用時(shí)注意將瓶蓋嚴(yán)。11 硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M 硼砂0.2M 硼砂pH0.05M 硼砂0.2M 硼酸文案大全（毫升）（毫升）7.41.09.07.61.58.57.82.08.08.03.07.0（毫升）（毫升）8.23.56.58.44.55.58.76.04.09.08.02.0硼砂Na2B4O7H2O,分子量=381.43;0.05M 溶液（=0.2M 硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M 溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。1

14、1 硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M 硼砂（毫升）0.2M 硼酸（毫升）7.41.09.07.61.58.57.82.08.08.03.07.0pH0.05M 硼砂（毫升）0.2M 硼酸（毫升）8.23.56.58.44.55.58.76.04.09.08.02.0硼砂 Na2B4O7 10H 2O,分子量=381.43;0.05M 溶液（=0.2M 硼酸根）含 19.07 克/升。硼酸H2BO3,分子量=61.84, 0.2M 溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。12 甘氨酸-氫氧化鈉緩沖液（0.05M ）X毫升0.2M甘氨酸+Y毫升0.2NnaO

15、H 加水稀釋至200毫升pHXY8.6504.08.8506.09.0508.89.25012.09.45016.8pHXY9.65022.49.85027.210.05032.010.45038.610.65045.5甘氨酸分子量=75.07;0.2M 溶液含15.01克/升。13 硼砂-氫氧化鈉緩沖液（0.05M 硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH 加水稀釋至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 Na2B4O7 10H2O,分子量=381.43;0.05M 溶液為 19

16、.07 克/升。14 .碳酸鈉-碳酸氫鈉緩沖液（0.1M）Ca2+、Mg 2+存在時(shí)不得使用pH0.1MNa 2CO3（毫升）0.1MN 2HCO 3（毫升）20 C37 C9.169.408.779.1212989.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na 2CO2 WH 2O 分子量=286.2;0.1M 溶液為 28.62 克/升。N2HCO3分子量=84.0;0.1M 溶液為8.40克/升。15 . “ PBS ”緩沖液PH7.67.47.27.0H2ONaClNazHPO

17、 4NaH 2PO410008.52.20.110008.52.20.210008.52.20.3100ml8.5g2.2g0.4g附錄四常用緩沖溶液的配制(一)磷酸氫二鈉-檸檬酸緩沖液0.2 mol/L0.1 mol/L0.2 mol/L0.1mol/LpHpHNa2HPO4檸檬酸(mL)Na2HPO4檸檬酸(mL)(mL)2.2).4019.602.41.2418.762.62.1817.822.83.1716.833.04.1115.893.24.9415.063.45.7014.303.65.4413.563.87.1012.904.07.7112.294.23.2811.724.43

18、.8211.184.69.3510.654.89.8610.145.010.309.70(mL)5.210.729.285.411.158.855.611.608.405.812.097.916.012.637.376.213.226.786.413.856.156.614.555.456.815.454.557.016.473.537.217.392.617.418.171.837.618.731.277.819.150.858.019.450.55Na2HPO4 分子量=141.98 ; 0.2 mol/L 溶液為 28.40 g/L。Na2HPO4 2H2O 分子量=178.05 ; 0

19、.2 mol/L 溶液為 35.61 g/L。Na2HPO4 12H2O 分子量=358.22 ; 0.2 mol/L 溶液為 71.64 g/L。C6H8O7 H2O 分子量=210.14 ; 0.1 mol/L 溶液為 21.01 g/L。(二)檸檬酸-檸檬酸鈉緩沖液(0.1 mol/L )pH0.1檸檬酸mol/L(mL)0.1 mol/L檸檬酸鈉(mL)pH0.1 mol/L檸檬酸(mL)0.1 mol/L檸檬酸鈉(mL)3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.8

20、14.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8檸檬酸：C6H8O7 H2O 分子量=210.14; 0.1 mol/L 溶液為 21.01 g/L。檸檬酸鈉：Na3C6H5O7 2H2O 分子量=294.12; 0.1 mol/L 溶液為 29.41 g/L。(三)醋酸-醋酸鈉緩沖液(0.2 mol/L )pH(18 C)0.2 mol/LNaAc (mL)0.2 mol/LHAc (mL)pH(18 C)0.2 mol/LNa

21、Ac (mL)0.2 mol/LHAc (mL)3.60.759.354.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.86.400.60NaAc 3H2O 分子量=136.09 ; 0.2 mol/L 溶液為 27.22 g/L。冰乙酸11.8 mL稀釋至1 L (需標(biāo)定)。(四)磷酸二氫鉀-氫氧化鈉緩沖液(0.05 mol/L )X 毫升0.2 mol/L KH2PO4+Y 毫升0.2 mol/L NaOH加水稀釋至

22、20毫升。pH (20 C)X (mL)Y (mL)pH (20 C)X (mL)Y (mL)5.850.3727.052.9636.050.5707.253.5006.250.8607.43.9506.451.2607.654.2805.651.7807.854.5206.852.3658.054.680(五)磷酸氫二鈉-磷酸二氫鈉緩沖液(0.2 mol/L )pH0.2 mol/L0.2 mol/LpH0.2 mol/L0.2 mol/LNa2HPO4(mL)NaH2PO4(mL)Na2HPO4(mL)NaH2PO4(mL)5.88.092.07.061.039.05.910.090.07

23、.167.033.05.012.387.77.272.028.05.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.051.08.094.75.36.955.045.0Na2HPO4 2H2O 分子量=178.05 ; 0.2 mol/L 溶液為 35.61 g/L。Na2HPO4 12H2O 分子量=358.22 ; 0.2 mol

24、/L 溶液為 71.64 g/L。NaH2PO4 H2O 分子量=138.01 ; 0.2 mol/L 溶液為 27.6 g/L。NaH2PO4 2H2O 分子量=156.03 ; 0.2 mol/L 溶液為 31.21 g/L。（六）巴比妥納-鹽酸緩沖液pH0.04 mol/L0.2 mol/LPH0.04 mol/L0.2 mol/L(18 C)巴比妥鈉(mL)HCl (mL)(18 C)巴比妥鈉(mL)HCl (mL)6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.6100

25、13.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21巴比妥鈉分子量=206.18 ; 0.04 mol/L 溶液為8.25 g/L(七) Tris-HCI 緩沖液(0.05 mol/L )50毫升0.1moI/L 三羥甲基氨基甲烷(Tris)溶液與X毫升0.1moI/L 鹽酸混勻并稀釋至 100毫升。pH (25 C)X (mL)pH (25 C)X (mL)7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2Tris分子量=121.14; 0.1 mol/L 溶液為12.114 g/L 。 Tri