可溶性CD40論文：血漿可溶性CD40和CD40L水平變化與子癇前期腎臟功能損害的相關(guān)性

1、可溶性CD40論文：血漿可溶性CD40和CD40L水平變化與子癇前期腎臟功能損害的相關(guān)性【中文摘要】探討血漿可溶性CD40(sCD40和可溶性CD40配體(SCD40L)在子癇前期患者中的水平變化及其與腎臟功能損傷的關(guān)系。方法選擇2008年8月-2010年6月在青島大學醫(yī)學院附屬醫(yī)院產(chǎn)科分娩的子癇前期孕婦63例,其中輕度子癇前期孕婦28例(輕度子癇前期組),重度子癇前期組35例(重度子癇前期組),同期另選妊娠結(jié)局良好的孕婦30例為對照組。采用酶聯(lián)免疫吸附法檢測3組孕婦血漿中SCD40和SCD40L的水平，同時檢測血常規(guī)、C反應(yīng)蛋白(CRP)、尿常規(guī)、24h尿蛋白定量，以及血清尿酸(UA)、肌酐

2、(Cr)、尿素氮(BUN)等生化指標。分析血漿SCD40和SCD40L的水平變化及其與臨床檢測結(jié)果的相關(guān)性。結(jié)果(1)重度子癇前期組SCD40和SCD40L分別為133.6(105.7-189.9)pg/ml和12.5(8.3-17.9)ng/ml,明顯高于輕度子癇前期組和對照組，兩組分別比較，差異均有統(tǒng)計學意義(PV0.01)；輕度子癇前期組SCD40和SCD40L分別為126.5(84.1-157.8)pg/ml和10.4(5.6-15.1)ng/ml,明顯高于對照組的90.7(62.3-122.4)pg/ml和4.4(1.5-8.7)ng/ml,分別比較，差異均有統(tǒng)計學意義(PV0.01

3、)。(2)子癇前期患者SCD40水平與24h尿蛋白定量呈正相關(guān)(r=0.434,P<0.05);與UACRP明顯呈正相關(guān)(r=0.536、0.528,P<0.01);與收縮壓、舒張壓、分娩孕齡、Cr、BUNPLT無明顯相關(guān)性(r=0.135、0.183>-0.133、0.190、0.167、-0.221,P>0.05)。子癇前期患者SCD40L水平與24h尿蛋白、UA,CRP勻呈明顯的正相關(guān)(r=0.591、0.445、0.539,P<0.01)；與收縮壓、舒張壓、分娩孕齡、Cr、BUNPLT無明顯相關(guān)性(r=0.178、0.212、-0.292、0.144、0.

4、135、-0.273,P>0.05)。血漿SCD40L與SCD40呈明顯正相關(guān)(r=0.707,P<0.01)。(5)正常對照組中血漿SCD40和SCD40L水平與各項觀察指標均無相關(guān)性(P>0.05)。結(jié)論子癇前期患者血漿SCD40和SCD40L水平明顯升高，可能參與子癇前期內(nèi)皮功能損傷的發(fā)病機制，并導致腎臟功能損傷?！居⑽恼縏oinvestigatethevarianeelevelsofplasmasolubleCD40(sCD40)andSolubleCD40ligand(sCD40L)inpreeelamptiepatientswithrenaldamageand

5、itsrelationship.MethodsAtotalof63pregnantwomendeliveredintheDepartmentofObstetrics,AffiliatedHospitalofQingdaoUniversityMedicalCollegefromAugust2008toJune2010wereineludedinthestudy.Amongthesewomen,28easesweremild(groupofmildpreeelampsia),and35easesweresevere(groupofseverepreeelampsia).30normotesivep

6、regnantwomenwereenrolledinthestudyastheeontrolgroupduringthesamestudyperiod.ExpressionofsCD40andsCD40LweredeterminedbyEnzyme-linkedimmunosorbentassay(ELISA)inthepregnantwomenofthe3groups.Atthesametime,thebloodroutine,Creaetionprotein(CRP),urineroutine,24hurineproteinexcretion,andserumuricacid(UA),cr

7、eatinine(Cr),bloodureanitrogen(BUN)weremeasuredtoinvestigatethecorrelationbetweentheSCD40/CD40Landthebloodbiochemicalindexesin3groupsofrecruitedwomen.Results(1)TheconcentrationofsCD40andsCD40Lwassignificantlyhigherinwomenwithseverepreeclampsiacomparedwithmildpreeclampsiaandcontrolgroups.Theplasmalev

8、elofsCD40andsCD40Lwas133.6(105.7-189.9)pg/mland12.5(8.3-17.9)ng/ml,26.5(84.1-157.8)pg/mland10.4(5.6-15.1)ng/ml,90.7(62.3-122.4)pg/mland4.4(1.5-8.7)ng/ml,respectively,inseverepreeclampsia,mildpreeclampsia,andcontrolgroup.SignificantstatisticsdifferencewasfoundinmultiplecomparisonPv0.01).(2)Thecorrela

9、tionanalysisindicatedthatthelevelofsCD40hasapositivecorrelationwith24hurineproteinexcretion(r=0.434,P<0.05),alsosignificantpositivecorrelation(r=0.536、0.528,P<0.01)betweenthelevelofsCD40andUA,CRPwasfoundinwomenwithpreeclampsia.TherewasnosignificantcorrelationbetweenthelevelofsCD40andsystolicbloodpressure,diastolicbloodpressure,deliverygestationala