Pseudothymidine-5-Methyl-2-Deoxypseudouridin-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPseudothymidineCat. No.: HY-101969CAS No.: 65358-15-8Synonyms: 5-Methyl-2-Deoxypseudouridin分式: CHNO分量: 242.23作靶點: Nucleoside Antimetabolite/Analog作通路: Cell Cycle/DNA Damage儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 mont

2、hs-20C 1 month溶解性數(shù)據體外實驗 DMSO : 61.17 mg/mL (252.53 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 4.1283 mL 20.6415 mL 41.2831 mL5 mM 0.8257 mL 4.1283 mL 8.2566 mL10 mM 0.4128 mL 2.0642 mL 4.1283 mL請根據產品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL

3、ACTIVITY物活性 Pseudothymidine是胸苷的 C-核苷 (C-nucleosid) 類似物。體外研究Pseudothymidine is a C-nucleoside analog of thymidine 1. The calculated G50/mod is -0.5 kcal/mol,with a Tm/mod of 0.82C. For the duplexes containing nine dA-T/T pairs, the Tm/mod is -0.9C and a G50/mod is +1.1 kcal/mol. The modification of t

4、he duplex containing 12 consecutive dA-T/T base pairs1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEproduces a Tm/mod of -0.9C and a G50/mod of +1.2 kcal/mol 2.PROTOCOLKinase Assay Thermal DNA duplex denaturation studies are performed with templates containing up to twelve consecutivedA residues t

5、hat are paired with its complement template containing consecutive T or Pseudothymidine (T)residues. Experiments are performed in a buffer (45 mM NaCl, 45 mM sodium citrate, pH 8.1, final vol. 1.5mL) containing template and its complement (1.5 M of each). Absorbance (260 nm) is monitored over arange

6、 of 25.0 to 90.0C with a change in temperature of 0.5C/min for five heating cycles. The initial heatingcycle is discarded and the Tm is determined by averaging the temperatures of the remaining four cycles. TheTm between similar duplexes is calculated by subtracting the Tm of the duplex containing s

7、tandard basesfrom the Tm of the duplex containing C-glycosides (including Pseudothymidine) 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. S Lutz, et al. An in vitro screening technique for DNA polymerases that can incorporate modified nu

8、cleotides. Pseudo-thymidine as asubstrate for thermostable polymerases. Nucleic Acids Res. 1999 Jul 1; 27(13): 2792-2798.2. Havemann SA, et al. Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplexstructure. Nucleosides Nucleotides Nucleic Acids. 2008 Mar;27(3):261-78.McePdfHeightCaution: Product has not been fully validated for medical application