L-Alanine-L-2-Aminopropionic-acid-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEL-AlanineCat. No.: HY-N0229CAS No.: 56-41-7Synonyms: L-2-Aminopropionic acid分式: CHNO分量: 89.09作靶點(diǎn): Endogenous Metabolite作通路: Metabolic Enzyme/Protease儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn)

2、 H2O : 150 mg/mL (1683.69 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 11.2246 mL 56.1230 mL 112.2460 mL5 mM 2.2449 mL 11.2246 mL 22.4492 mL10 mM 1.1225 mL 5.6123 mL 11.2246 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 L-Alanine種必需氨 酸，能夠參

3、與糖和酸的代謝，增強(qiáng)免疫，并能為肌組織、腦和中樞神經(jīng)系統(tǒng)提供能量。IC50 & Target Human Endogenous Metabolite體外研究The viability of both hiPSCs, 201B7 cells and ehiPSCs decrease with an increase in L-Alanine concentration,1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEand reach 7.51.3% and 3.70.7% respectively at 1.2 M of L-Alanine.

4、On the other hand, no decrease in theviability of hFBs and hSkMCs are observed. Although the viability of iCMs slightly decreases along with theincrease of the L-Alanine concentration, viability of iCMs at 1.2 M concentration of L-Alanine, 49.46.9%, issignificantly higher than that of undifferentiat

5、ed iPSCs, 201B7 cells and ehiPSCs (p 1.PROTOCOLCell Assay To investigate the selective removal of hiPSCs from differentiated cells by the high L-Alanine medium, twotypes of hiPSCs, 201B7 hiPSCs (201B7 cells) and an hiPSC line derived by episomal system (ehiPSCs) areused, along with normal human derm

6、al fibroblasts (hFBs), human skeletal muscle cells (hSkMCs) andhiPSC-derived cardiomyocytes (iCMs) as differentiated cells. The cells are incubated in a mediumsupplemented with L-Alanine at various concentrations (0-1.2 M) or treatment times (1-24 h). The medium isreplaced with a normal medium and t

7、he relative cell viability is measured after 24 h 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Nagashima T, et al. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine.Sci Rep. 2018 Aug 20;8(1):12427.McePdfHeightCaution: Product has not been fully validated for medical applications.For research