3-3-Dihexyloxacarbocyanine-iodide-DiOC6-3-iodide-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE3,3-Dihexyloxacarbocyanine iodideCat. No.: HY-D0084CAS No.: 53213-82-4Synonyms: DiOC6(3) iodide分式: CHINO分量: 572.52作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 125 m

2、g/mL (218.33 mM)H2O : 0.1 mg/mL (insoluble)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.7467 mL 8.7333 mL 17.4666 mL5 mM 0.3493 mL 1.7467 mL 3.4933 mL10 mM 0.1747 mL 0.8733 mL 1.7467 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性

3、3,3-Dihexyloxacarbocyanine iodide種羰花 染料, 可于檢測線粒體膜電位的變化。體外研究Addition of cells to the cuvette containing 0.25 M 3,3-Dihexyloxacarbocyanine iodide (DiOCg(3) leads toan increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is1/2 Master of Small Molecules

4、您邊的抑制劑師www.MedChemEadded to neutrophils preequilibrated with 3,3-Dihexyloxacarbocyanine iodide, there is a short lag period of 5seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demon

5、strates that inresting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds tothe distribution of mitochondria 1.PROTOCOLCell Assay 1 Neutrophils (2.5106/mL) are preequilibrated in 0.25 M 3,3-Dihexyloxacarbocyanine iodide (DiOCg(3) for 5min at 37C in a 1 c

6、m path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in aspectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by asyringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v) 1.MCE has

7、 not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Korchak HM, et al. A carbocyanine dye, DiOC6(3), acts as a mitochondrial probe in human neutrophils. Biochem Biophys Res Commun.1982 Oct 29;108(4):1495-501.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use