DMA-trihydrochloride - Dye Reagent - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDMA trihydrochlorideCat. No.: HY-15621ACAS No.: 2095832-33-8分式: CHClNO分量: 577.93作靶點: Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 15.9 mg/mL (27.51 mM; Need ultrasonic an

2、d warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.7303 mL 8.6516 mL 17.3031 mL5 mM 0.3461 mL 1.7303 mL 3.4606 mL10 mM 0.1730 mL 0.8652 mL 1.7303 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 DMA trihydrochloride種熒光化合物 (ex=340 nm, em=478 nm)。IC50 & Target IC50: 3.4

3、 M (HeLa cell), 5.3 M (MCF7 cell) 1體外研究The newly synthesized bisbenzimidazole derivatives DMA (6c) is evaluated for their cytotoxicity againsthuman tumor cell lines, which are cervix carcinoma cell line (HeLa), breast carcinoma cell line (MCF7) andbrain glioma cell line (U87) in comparison to Hoechs

4、t. In case of MCF7, the IC50 is observed at 5.3 M for1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEDMA. The IC50 determined in the case of HeLa is 3.4 M for DMA 1.PROTOCOLCell Assay Various human tumor cells (U87, HeLa and MCF7) are maintained as monolayer at 37C in 5% CO2 usingDMEM medium. Appro

5、ximately 3000-8000 cells/well are seeded in 96-well plates containing 200 L ofmedium and incubated for 24 h. The culture medium is replaced by fresh medium containing 1, 10, 50, 100 M of DMA (6c) and incubated for 24, 48 and 72 h. The cell viability is determined by the MTT assay. The lightabsorbanc

6、e is measured using a microplate reader 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur JMed Chem. 2011 Feb;46(2):659-69.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 4